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pNapin-dsRED

Plasmid

#127798

Purpose

Napin Promoter driven dsRED expression

Depositor

Scott Poethig

Article

Wu et al Genetics. 2015 May;200(1):35-45. doi: 10

Type

Empty backbone

Expression

Plant

Available Since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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pBabe-TetCMV-puro-mVenus-PA-Rac1-C450A

Plasmid

#22023

Depositor

Klaus Hahn

Article

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

mVenus-PA-Rac1-C450A (RAC1 Human, Avena sativa (oat))

Use

Retroviral

Expression

Mammalian

Mutation

The LOV domain contains the mutation C450A.Rac1 s…

Available Since

Sept. 29, 2009

Availability

Academic Institutions and Nonprofits only
pET28a-PKM2-R399E

Plasmid

#122691

Purpose

Express His-tagged PKM2 (R399E) protein in bacteria.

Depositor

Wen-Ching Wang

Article

Chen et al Commun Biol. 2019 Mar 15;2:105. doi: 10.

Insert

Pyruvate kinase M2 isoform (PKM Human)

Tags

6His-tag

Expression

Bacterial

Mutation

R399E

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a-PKM2-G415R

Plasmid

#122693

Purpose

Express His-tagged PKM2 (G415R) protein in bacteria.

Depositor

Wen-Ching Wang

Article

Chen et al Commun Biol. 2019 Mar 15;2:105. doi: 10.

Insert

Pyruvate kinase M2 isoform (PKM Human)

Tags

6His-tag

Expression

Bacterial

Mutation

G415R

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pFastBac HTB MBP-SARM1 A600G

Plasmid

#195764

Purpose

Purification of SARM1 from insect cells

Depositor

Hao Wu

Insert

SARM1 (SARM1 Human)

Tags

HIS-MBP-TEV

Expression

Insect

Mutation

A600G

Promoter

polyhedrin

Available Since

Feb. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pFA6a- mEGFP-kanMX6

Plasmid

#105146

Purpose

Yeast gene targeting

Depositor

Thomas Pollard, Jian-Qiu Wu

Article

Wu et al Dev Cell. 2003 Nov;5(5):723-34.

Insert

mEGFP-kanMX6

Tags

mEGFP

Expression

Bacterial and Yeast

Mutation

A206K

Available Since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pCB301TOR-GFP

Plasmid

#164799

Purpose

Expression of GFP in oomycetes using Agrobacterium-mediated transformation

Depositor

Miaoying Tian

Article

Wu et al BMC Microbiol. 2016 Sep 6;16(1):204. doi

Insert

GFP

Use

Oomycete expression

Available Since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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pET28-OmpT

Plasmid

#68862

Purpose

construct for expression of OmpT protease in the pET28a plasmid using the restriction sites NcoI and EcoRI.

Depositor

Neil Kelleher

Article

Wu et al Nat Methods. 2012 Jun 17;9(8):822-4. doi

Insert

OmpT (ompT )

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pN3-pHluorinCAAX

Plasmid

#123313

Purpose

Expresses the proton pump rhodopsin in mammalian cells under CMV promoter

Depositor

Yulong Li

Article

Wu et al Elife. 2019 Jan 14;8. pii: 43366. doi: 1

Insert

pHluorinCAAX

Expression

Mammalian

Promoter

CMV

Available Since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

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pDisplay-Gncp-iGluSnFR

Plasmid

#107337

Purpose

Noncircularly permutated (ncp) variant of iGluSnFR. Membrane-displayed.

Depositor

Robert Campbell

Article

Wu et al ACS Chem Biol. 2018 Jan 22. doi: 10.1021

Insert

GltI based and noncircularly permutated glutamate biosensor

Expression

Mammalian

Promoter

CMV

Available Since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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pBabe-TetCMV-puro-mCerulean-PA-Rac1

Plasmid

#22031

Depositor

Klaus Hahn

Article

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

mCerulean-PA-Rac1 (RAC1 Human, Avena sativa (oat))

Use

Retroviral

Expression

Mammalian

Mutation

Rac1 starts at I4 and contains mutations Q61L, E9…

Available Since

Sept. 29, 2009

Availability

Academic Institutions and Nonprofits only
pLenti-GRET

Plasmid

#158222

Purpose

Control lentiviral plasmid encoding GFP-Nluc BRET reporter from Schaub et al., Cancer Research, 2015.

Depositor

Charles P. Lai

Article

Wu et al Adv Sci (Weinh). 2020 Aug 16;7(19):20014

Insert

GFP-Nluc

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-DIO-GRAB_Ado1.0med

Plasmid

#153292

Purpose

Express the medium-affinity adenosine sensor GRAB_Ado1.0med in Cre positive cells

Depositor

Yulong Li

Article

Wu et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

Insert

Adenosine sensor GRAB_Ado1.0med (medium affinity)

Use

AAV

Promoter

EFS

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-Rab7Q67L

Plasmid

#169038

Purpose

Express human constitutively active form of Rab7a(Q67L) in mammalian cells

Depositor

Gia Voeltz

Article

Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

Insert

Rab7a (RAB7A Human)

Tags

acGFP1

Expression

Mammalian

Mutation

Q67L

Promoter

CMV

Available Since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pCB301TOR

Plasmid

#164798

Purpose

Oomycete expression vector using Agrobacterium-mediated transformation

Depositor

Miaoying Tian

Article

Wu et al BMC Microbiol. 2016 Sep 6;16(1):204. doi

Insert

NPTII

Use

Oomycete expression

Available Since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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Tom20-mCh-FKBP

Plasmid

#162436

Purpose

for use in CID (chemically induced dimerization) or CIT (chemically induced trimerization) system

Depositor

Takanari Inoue

Article

Wu et al Nat Methods. 2020 Sep;17(9):928-936. doi

Insert

Tom20-mCh-FKBP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Tornado-F30-TAR Variant-1

Plasmid

#129406

Purpose

circular TAR Variant-1

Depositor

Samie Jaffrey

Article

Wu et al Nat Methods. 2019 Sep;16(9):862-865. doi

Insert

circular F30-TAR Variant-1

Expression

Mammalian

Promoter

U6

Available Since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pFA6a- mCFP-kanMX6

Plasmid

#105145

Purpose

Yeast gene targeting

Depositor

Thomas Pollard, Jian-Qiu Wu

Article

Wu et al Dev Cell. 2003 Nov;5(5):723-34.

Insert

mCFP-kanMX6

Tags

mCFP

Expression

Bacterial and Yeast

Mutation

A206K

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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tetO-Bcl2-IRES-tdtomato(TET005)

Plasmid

#117857

Purpose

This plasmid expresses Bcl2 under the activation of tTA trans-activator along with fluorescent marker tdTomato.

Depositor

C. Ron Yu

Article

Wu et al Neuron. 2018 Dec 5;100(5):1066-1082.e6.

Insert

Bcl2 (Bcl2 Mouse)

Use

Tta/teto

Expression

Mammalian

Promoter

tetO

Available Since

May 30, 2019

Availability

Academic Institutions and Nonprofits only

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pET29b-rhMOG

Plasmid

#225484

Purpose

Express the soluble domain of Myelin Oligodendrocyte Glycoprotein (rhMOG) in SHuffle E. coli

Depositor

John Pak

Article

Wu et al J Immunol Methods. 2024 Oct 13:113764. d

Insert

Soluble Domain of Myelin Oligodendrocyte Glycoprotein (MOG Human)

Tags

6xHis tag

Expression

Bacterial

Mutation

deleted amino acids 1-29 (signal peptide) and 150…

Promoter

T7

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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