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pETCON-HB71/design_10

Plasmid

#30288

Depositor

David Baker

Article

Fleishman et al J Mol Biol. 2011 Sep 29.

Insert

SC1918/H1 HA designed binding protein HB71

Expression

Yeast

Available Since

June 8, 2011

Availability

Academic Institutions and Nonprofits only
pETCON-HB72/design_31

Plasmid

#30289

Depositor

David Baker

Article

Fleishman et al J Mol Biol. 2011 Sep 29.

Insert

SC1918/H1 HA designed binding protein HB72

Expression

Yeast

Available Since

June 8, 2011

Availability

Academic Institutions and Nonprofits only
pETCON-HB73/design_74

Plasmid

#30290

Depositor

David Baker

Article

Fleishman et al J Mol Biol. 2011 Sep 29.

Insert

SC1918/H1 HA designed binding protein HB73

Expression

Yeast

Available Since

June 8, 2011

Availability

Academic Institutions and Nonprofits only
pETCON-HB74/design_56

Plasmid

#30291

Depositor

David Baker

Article

Fleishman et al J Mol Biol. 2011 Sep 29.

Insert

SC1918/H1 HA designed binding protein HB74

Expression

Yeast

Available Since

June 8, 2011

Availability

Academic Institutions and Nonprofits only
pETCON-HB75/design_18

Plasmid

#30292

Depositor

David Baker

Article

Fleishman et al J Mol Biol. 2011 Sep 29.

Insert

SC1918/H1 HA designed binding protein HB75

Expression

Yeast

Available Since

June 8, 2011

Availability

Academic Institutions and Nonprofits only
pETCON-HB76/design_13

Plasmid

#30293

Depositor

David Baker

Article

Fleishman et al J Mol Biol. 2011 Sep 29.

Insert

SC1918/H1 HA designed binding protein HB76

Expression

Yeast

Available Since

June 8, 2011

Availability

Academic Institutions and Nonprofits only
pETCON-HB77/design_47

Plasmid

#30294

Depositor

David Baker

Article

Fleishman et al J Mol Biol. 2011 Sep 29.

Insert

SC1918/H1 HA designed binding protein HB77

Expression

Yeast

Available Since

June 8, 2011

Availability

Academic Institutions and Nonprofits only
pETCON-HB78/design_44

Plasmid

#30295

Depositor

David Baker

Article

Fleishman et al J Mol Biol. 2011 Sep 29.

Insert

SC1918/H1 HA designed binding protein HB78

Expression

Yeast

Available Since

June 8, 2011

Availability

Academic Institutions and Nonprofits only
pETCON-HB81/design_35

Plasmid

#30297

Depositor

David Baker

Article

Fleishman et al J Mol Biol. 2011 Sep 29.

Insert

SC1918/H1 HA designed binding protein HB81

Expression

Yeast

Available Since

June 8, 2011

Availability

Academic Institutions and Nonprofits only
pETCON-HB82/design_21

Plasmid

#30298

Depositor

David Baker

Article

Fleishman et al J Mol Biol. 2011 Sep 29.

Insert

SC1918/H1 HA designed binding protein HB82

Expression

Yeast

Available Since

June 8, 2011

Availability

Academic Institutions and Nonprofits only
pETCON-HB7/design_52

Plasmid

#30299

Depositor

David Baker

Article

Fleishman et al J Mol Biol. 2011 Sep 29.

Insert

SC1918/H1 HA designed binding protein HB7

Expression

Yeast

Available Since

June 8, 2011

Availability

Academic Institutions and Nonprofits only
pETCON-HB9/design_41

Plasmid

#30300

Depositor

David Baker

Article

Fleishman et al J Mol Biol. 2011 Sep 29.

Insert

SC1918/H1 HA designed binding protein HB9

Expression

Yeast

Available Since

June 8, 2011

Availability

Academic Institutions and Nonprofits only
pETCON-IgG3/design_30

Plasmid

#30302

Depositor

David Baker

Article

Fleishman et al J Mol Biol. 2011 Sep 29.

Insert

IgG Fc Designed Binder IgG3

Expression

Yeast

Available Since

June 8, 2011

Availability

Academic Institutions and Nonprofits only
pETCON-IgG9/design_59

Plasmid

#30303

Depositor

David Baker

Article

Fleishman et al J Mol Biol. 2011 Sep 29.

Insert

IgG Fc Designed Binder IgG9

Expression

Yeast

Available Since

June 8, 2011

Availability

Academic Institutions and Nonprofits only
pDEST40-DJ1-D149A

Plasmid

#29406

Depositor

Mark Cookson

Insert

DJ1 (PARK7 Human)

Use

Gateway Cloning

Tags

6XHis and V5

Expression

Mammalian

Mutation

Asp149Ala

Available Since

March 21, 2011

Availability

Academic Institutions and Nonprofits only
pDest40 DHFR.dn-cHSF1

Plasmid

#134735

Purpose

Mammalian expression vector for constitutively active dominant-negative HSF1 fused to DHFR destabilized domain

Depositor

Matthew D. Shoulders

Article

Moore et al ACS Chem Biol. 2016 Jan 15;11(1):200-10.

Insert

DHFR.dn-cHSF1 (HSF1 Human)

Tags

DHFR

Expression

Mammalian

Mutation

Deletion of amino acids 186-202, 379−529

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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MSCV-C 18E6

Plasmid

#37884

Depositor

Karl Munger

Article

Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

Insert

HPV 18E6 (E6 HPV)

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Promoter

PKG

Available Since

July 26, 2012

Availability

Academic Institutions and Nonprofits only
Green monkey (Chlorocebus sabaeus) sgRNA library

Pooled Library

#178284

Purpose

Knockout library targeting the green monkey (Chlorocebus sabaeus) genome.

Depositor

John Doench, David Root

Article

Wei et al Cell. 2021 Jan 7;184(1):76-91.e13. doi:

Expression

Mammalian

Species

Chlorocebus sabaeus (african green monkey)

Use

CRISPR and Lentiviral

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only
Mouse Toronto KnockOut (mTKO) CRISPR Library - lentiCRISPRv2

Pooled Library

#159392

Purpose

Mouse lentiviral genome-wide CRISPR library

Depositor

Jason Moffat

Article

Lawson et al Nature. 2020 Oct;586(7827):120-126. doi:

Expression

Mammalian

Species

Mus musculus

Use

CRISPR and Lentiviral

Available Since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only
pSpCas9(BB)-T2A-HygR

Plasmid

#118153

Purpose

SpCas9 expression vector

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

hSpCas9-T2A-HygR

Use

CRISPR

Tags

3XFLAG

Expression

Mammalian

Promoter

CAG and U6

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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piggyBac-rtTA (4th_Gen)-NFIB-SOX9-IRES-mKate2 (VK_1244)

Plasmid

#209082

Purpose

NFIB and SOX9 mediated astrocye induction

Depositor

Vikram Khurana

Article

Lam et al Neuron. 2024 Sep 4;112(17):2886-2909.e16

Inserts

NFIB-P2A-SOX9-V5 (NFIB, SOX9 Human)
mKate2

Expression

Mammalian

Promoter

TRE4G

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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MSCV-C 16E6

Plasmid

#37876

Depositor

Karl Munger

Article

Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

Insert

HPV 16E6 (E6 HPV)

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Promoter

PKG

Available Since

July 25, 2012

Availability

Academic Institutions and Nonprofits only
pAAV-CAG-FLEx-iSeroSnFR

Plasmid

#128486

Purpose

Fluorescent reporter for serotonin (conditional + membrane localization tag)

Depositor

Lin Tian

Article

Unger et al Cell. 2020 Dec 12. pii: S0092-8674(20)31

Has Service

AAV9

Insert

iSeroSnFR

Use

AAV

Tags

Ig-kappa leader, Myc, PDGFR, and cpsfGFP

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
MTK Lentivirus Multi Cassette Destination Vector (pAN2424)

Plasmid

#194221

Purpose

Backbone for constructing multi casette expression plasmids using the Mammalian Tookit

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv [Preprint]. 2021 Apr 20:2021.04.

Type

Empty backbone

Use

Lentiviral

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pEF5/FRT-DEST EVC2dW-TEV-YFP-Flag

Plasmid

#41006

Depositor

Rajat Rohatgi

Article

Dorn et al Dev Cell. 2012 Sep 12. pii: S1534-5807(1

Insert

Ellis van Creveld syndrome 2 (EVC2 Mouse)

Tags

TEV-YFP-Flag

Expression

Mammalian

Mutation

deleted amino acids 1177-1220

Available Since

Nov. 15, 2012

Availability

Academic Institutions and Nonprofits only
Tgfb-Induced-Enhancer:Beta-globin-minimal-promoter-EGFP:pA pDestTol2pA2

Plasmid

#220508

Purpose

TGFb-inducible fluorescent reporter (TIE:EGFP)

Depositor

Leonard Zon

Inserts

TGFb inducible enhancer
beta-globin minimal promoter
SV40 PolyA signal

Use

Zebrafish expression (tol2)

Tags

EGFP

Available Since

June 11, 2024

Availability

Academic Institutions and Nonprofits only

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pRRL-pEF-H2B-mCherry-T2A-rTetR-Dest-SV40

Plasmid

#186968

Purpose

Lentiviral destination vector for cloning desired protein as rTetR fusion for chromatin regulation reporter (pEF-H2B-mCherry-T2A-rTetR, with SV40) for expression in mammalian cells.

Depositor

Brian Liau

Article

Lue et al Nat Chem Biol. 2023 Feb;19(2):176-186. d

Insert

pEF-H2B-mCherry-T2A-rTetR-Dest-SV40

Use

Lentiviral

Tags

rTetR fusion

Expression

Mammalian

Promoter

EF-1alpha promoter

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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MSCV-N EBNALP

Plasmid

#37959

Depositor

Karl Munger

Article

Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

Insert

EBNALP (EBNA-LP H. Herpesvirus 4 (EBV))

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Promoter

PKG

Available Since

Aug. 2, 2012

Availability

Academic Institutions and Nonprofits only
MSCV-N 16E7

Plasmid

#37881

Depositor

Karl Munger

Article

Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

Insert

HPV 16 E7 (E7 HPV)

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Promoter

PKG

Available Since

July 26, 2012

Availability

Academic Institutions and Nonprofits only
MSCV-N SV40 LT

Plasmid

#37857

Depositor

Karl Munger

Article

Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

Insert

SV40 LT (SV40gp6 Simian Virus 40)

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Promoter

PKG

Available Since

July 23, 2012

Availability

Academic Institutions and Nonprofits only

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