Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 12,979 results for: NUC

Clear keywords
Showing: 7381 - 7400 of 12979 results

  8. pS44i8GH-1

    Plasmid
    #207525
    Purpose
    Conditionally-replicating in Pseudomonas vector, high-copy-number; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→repA, P14b with a translational BCD2 coupler from BG35 (53)→msfGFP; SmR/SpR
    Depositor
    Pablo Ivan Nikel
    Article
    Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.
    Insert
    oriV(pRO1600/ColE1), xylS, Pm→repA, P14b with a translational BCD2 coupler from BG35 (53)→msfGFP;
    Use
    CRISPR; Pseudomonas vector
    Available Since
    Dec. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    207525-plasmid-map-sequence-id-409596

    Enlarge

  12. eA3Ai-max

    Plasmid
    #207166
    Purpose
    Express A3A with an N57G point mutation and an intron in a BE4max-based base editor
    Depositor
    Reuben Harris
    Article
    Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10
    Insert
    Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3A (APOBEC3A Human)
    Tags
    Cas9-UGI-UGI-NLS and NLS
    Expression
    Mammalian
    Mutation
    N57G
    Promoter
    CMV
    Available Since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    207166-plasmid-map-sequence-id-409514

    Enlarge

  16. pCDNA5/FRT/TO–γ-tubulinΔGTP-RNAi-resistant-GFP

    Plasmid
    #205604
    Purpose
    Tet-inducible expression of N229A gamma-tubulin in human cells, harbors silent mutations in shRNA target site, FLP-In mediated insertion
    Depositor
    Tarun Kapoor
    Article
    Berman et al J Cell Biol. 2023 Mar 6;222(3):e20220410
    Insert
    γ-tubulinΔGTP-RNAi-resistant-GFP
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    G2228A, C2231T, C2237T, G2240A, N229A
    Available Since
    Aug. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    205604-plasmid-map-sequence-id-405873

    Enlarge

  17. pYG215

    Plasmid
    #200837
    Purpose
    Transcribes a glmZ' (1-146)-gfp fusion. gfp can be replaced via AgeI/XbaI-sites with gene of interest to release its RNA with a 5' monophosphate upon cleavage.
    Depositor
    Boris Görke
    Article
    Schilder et al Nucleic Acids Res. 2023 Mar 29:gkad226.
    Insert
    glmZ (glmZ Escherichia coli str. K-12 substr. MG1655)
    Use
    Synthetic Biology
    Tags
    GFP
    Expression
    Bacterial
    Promoter
    P-LlacO-1
    Available Since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    200837-plasmid-map-sequence-id-405511

    Enlarge

  18. pASPIre4

    Plasmid
    #196656
    Purpose
    Derivative of pASPIre3. Contains SpeI restriction site within the CDS of bxb1 to enable diversification of the 5’-UTR and codons 2-16.
    Depositor
    Markus Jeschek
    Article
    Hollerer et al Nucleic Acids Res. 2023 Mar 21;51(5):237
    Insert
    Bxb1-sfGFP fusion controlled by rhamnose promoter; attB/attP-flanked discriminator; exchangable 5'-UTR and CDS (codons 1-16)
    Expression
    Bacterial
    Mutation
    SpeI site in Bxb1 CDS
    Promoter
    rhamnose-inducible promoter
    Available Since
    Aug. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    196656-plasmid-map-sequence-id-403168

    Enlarge

  20. pJUMP24_T24_pRham-ilvA-relE_RBS3_CB2

    Plasmid
    #201533
    Purpose
    Expressing synthetic overlapping gene, ilvA-relE with internal RBS modification. Evolved strain with lower ilvA-relE expression. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)
    Depositor
    Dan Park
    Article
    Chlebek et al Nucleic Acids Res. 2023 Jun 1:gkad484. d
    Insert
    ilvA-relE_RBS3_CB2
    Use
    Synthetic Biology
    Mutation
    rhaR L128+1bp (frameshift), ilvA G455C
    Promoter
    PrhaBAD (rhamnose)
    Available Since
    June 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    201533-plasmid-map-sequence-id-398133

    Enlarge

Showing: 7381 - 7400 of 12979 results