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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 7,944 results for: RAP

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Showing: 7381 - 7400 of 7944 results

  4. Epac-S-H127

    Plasmid
    #170343
    Purpose
    6xHis_mT2Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Insert
    6xHis_mT2Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) (RAPGEF3 Human)
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A ā€¦
    Promoter
    CMV
    Available Since
    Oct. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    170343-plasmid-map-sequence-id-344642

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  5. Epac-S-H151

    Plasmid
    #170346
    Purpose
    6xHis_mT2Del_EPACdDEPCD_Q270E_cp174Cit biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Insert
    6xHis_mT2Del_EPACdDEPCD_Q270E_cp174Cit (RAPGEF3 Human)
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A ā€¦
    Promoter
    SP6
    Available Since
    Aug. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    170346-plasmid-map-sequence-id-336135

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  8. AAVS1-DRGFP

    Plasmid
    #113193
    Purpose
    DR-GFP substrate (Addgene #26475) flanked by human AAVS1 homology arms. Endogenous AAVS1 promoter drives T2A-hygromycin expression after integration. DR-GFP cassette contains PGK-puromycin.
    Depositor
    Adam Karpf
    Insert
    DR-GFP
    Use
    Donor construct with t2a-hygromycin for gene trapā€¦
    Expression
    Mammalian
    Available Since
    Oct. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    113193-plasmid-map-sequence-id-216317

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  9. pJD427

    Plasmid
    #154371
    Purpose
    Expresses FKBP12-XB and SspBcore-FRB from constitutive promoters
    Depositor
    Joseph Davis
    Article
    Davis et al ACS Chem Biol. 2011 Nov 18;6(11):1205-13
    Insert
    proB-RBS(B0032)-SspBcore-FRB-terminator(B0011)-proC-RBS(B0032)-FKBP12-sspBXB-terminator(B0011)
    Expression
    Bacterial
    Available Since
    Sept. 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    154371-plasmid-map-sequence-id-311827

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  13. 5KO9 (HMCES)

    Plasmid
    #101877
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    HMCES (HMCES Human)
    Tags
    6x His (modified C-term tag is AENLYFQSHHHHHH)
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 30, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    101877-plasmid-map-sequence-id-194533

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Showing: 7381 - 7400 of 7944 results