We narrowed to 9,493 results for: pho;

1. pAAV-syn-jYCaMP1s

   Plasmid

   #135424

   Purpose

   Yellow protein calcium sensor expressed under human Synapsin1 promoter, for AAV production or direct transfection, slow variant

   Depositor

   Kaspar Podgorski

   Article

   Mohr et al Nat Methods. 2020 May 25. pii: 10.1038/s

   Has Service

   AAV1

   Insert

   jYCaMP1s
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   Jan. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. iSH-iRFP670-sspB

   Plasmid

   #176102

   Purpose

   Heterodimerization with iLID, phosphatidylinositol 3-kinase derived iSH

   Depositor

   Dorus Gadella

   Article

   Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

   Insert

   phosphatidylinositol 3-kinase (Pik3r1 Mouse)
   

   Expression

   Mammalian

   Available Since

   March 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. LentiV_Neo_SIK3_CR_T221E

   Plasmid

   #108108

   Purpose

   Lentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation & TE mutation at LKB1 phosphorylation site) with neomycin resistance gene

   Depositor

   Christopher Vakoc

   Article

   Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

   Insert

   SIK3 (SIK3 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   change guanine 501 to cytosine (silent mutation),…

   Promoter

   EFS promoter

   Available Since

   April 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGem-nHyPer:cAPX

   Plasmid

   #84736

   Purpose

   Simultaneous expresion of stromal HyPer2 and cytosolic Ascorbate peroxidase (APX) in plant cells

   Depositor

   Philip Mullineaux

   Article

   Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

   Inserts

   APX1 (APX1 Mustard Weed)
   HyPer2 targeted to nucleus
   

   Tags

   NLS-SV40

   Expression

   Plant

   Promoter

   pFMV/p35S

   Available Since

   June 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLC-Flag-CSNK1A1 G40N-Puro

   Plasmid

   #123320

   Purpose

   Lentiviral vector for expression of Flag tagged CK1alpha G40N-P2A-Puro casette from a CMV promoter

   Depositor

   Eva Gottwein

   Article

   Patil et al Blood. 2018 Aug 9;132(6):577-586. doi: 1

   Insert

   CSNK1A1 (CSNK1A1 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   changed Glycine 40 to Asparagine

   Promoter

   CMV

   Available Since

   March 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. nCre-ST-NLS-P2A-NLS-pSC-cCre

   Plasmid

   #207638

   Purpose

   A plasmid encoding N-terminal Cre fused to SpyTag (ST) and photocaged SpyCatcher (pSC) fused to C-terminal Cre with a nuclear localization signal (NLS) sequence

   Depositor

   Cole DeForest

   Article

   Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

   Insert

   nCre-SpyTag-NLS-P2A-NLS-photocaged SpyCatcher-cCre
   

   Expression

   Mammalian

   Mutation

   Amber stop codon at SpyCatcher’s critical lysine

   Promoter

   CMV

   Available Since

   Oct. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. LENTICRISPR-HK2_sgRNA1

   Plasmid

   #201596

   Purpose

   CRISPR/Cas9-mediated gene knock-out

   Depositor

   Alexis Jourdain, Vamsi Mootha

   Article

   Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

   Insert

   HK2 (HK2 Human)
   

   Use

   CRISPR and Lentiviral

   Available Since

   July 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.3−3xFLAG−AGO2(S387A)

   Plasmid

   #220462

   Purpose

   Express S387 phospho-null mutant human AGO2 with N-terminal 3xFLAG tag in mammalian cells

   Depositor

   David Bartel

   Article

   Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

   Insert

   Human AGO2 (AGO2 Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Serine 387 to alanine

   Promoter

   CMV

   Available Since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. p3F-Strep-mBCL10

   Plasmid

   #33314

   Depositor

   Vanessa Redecke

   Article

   Stempin et al J Biol Chem. 2011 Oct 28;286(43):37147-5

   Insert

   Bcl10 (Bcl10 Mouse)
   

   Tags

   3xFLAG and StrepOne

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Dec. 21, 2011

   Availability

   Academic Institutions and Nonprofits only

10. pLC-CUL4A K705R-P2A-Puro

    Plasmid

    #124305

    Purpose

    Lentiviral vector for expression of Nedd8 attachment mutant K705R CUL4A-P2A-Puro casette from a CMV promoter

    Depositor

    Eva Gottwein

    Article

    Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

    Insert

    CUL4A (CUL4A Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    changed Lysine 705 to Arginine

    Promoter

    CMV

    Available Since

    July 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEB1N-mScarlet-LZ-LOV2

    Plasmid

    #197419

    Purpose

    Mammalian expression of the mScarlet-tagged N-terminal half of photo-inactivated π-EB1

    Depositor

    Torsten Wittmann

    Article

    Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

    Insert

    MAPRE1 (MAPRE1 Human)
    

    Tags

    A. sativa phototropin 1 LOV2 domain, GCN4 leucine…

    Expression

    Mammalian

    Mutation

    EB1 aa 1-185; resistant to EB1 shRNA#3 (Addgene #…

    Available Since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. M3-PSCFP2

    Plasmid

    #114185

    Purpose

    studying clustering of M3 receptors by superresolution microscopy (PALM)

    Depositor

    Aleksandra Radenovic

    Article

    Scarselli et al J Biol Chem. 2012 May 11;287(20):16768-8

    Insert

    Muscarin acetylcholine receptor 3 (CHRM3 Human)
    

    Tags

    3xHA and PS-CFP2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. LUC2 (L/Sec/deltaP)

    Plasmid

    #112145

    Purpose

    Mammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activity

    Depositor

    Paul Copeland

    Article

    Mehta et al J Biol Chem. 2004 Sep 3;279(36):37852-9.

    Insert

    Luciferase coding region with Cysteine 258 mutated to selenocysteine (U) and deleted AUGA in PHGPx SECIS core (Gpx4 Rat, Photinus pyralis)
    

    Expression

    Mammalian

    Mutation

    Cysteine 258 mutated to selenocysteine (U) and d…

    Available Since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. XE237 Human LEF-1 pCS2+

    Plasmid

    #16709

    Depositor

    Randall Moon

    Article

    Li et al J Biol Chem. 2004 Jan 23;279(4):2873-84.

    Insert

    Human LEF-1 (LEF1 Human)
    

    Use

    Xenopus expression

    Available Since

    March 14, 2008

    Availability

    Academic Institutions and Nonprofits only

15. pCSCRET2(#323)

    Plasmid

    #184058

    Purpose

    cyclofen-inducible CRE activation via mammalian cell transfection or mRNA synthesis

    Depositor

    Sophie Vriz

    Article

    Sinha et al Zebrafish. 2010 Jun;7(2):199-204. doi: 1

    Insert

    6myc-CRE-ERT2
    

    Use

    Synthetic Biology

    Tags

    6xMyc

    Expression

    Mammalian

    Mutation

    ERT2: G400V, M543A, L544A, deleted 1-281;

    Promoter

    sCMV+SP6

    Available Since

    July 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-ALK2 K235R

    Plasmid

    #80872

    Purpose

    mammalian expression of ALK2 K235R

    Depositor

    Aristidis Moustakas

    Article

    Raja et al Oncotarget. 2016 Jan 12;7(2):1120-43. do

    Insert

    ALK2 (ACVR1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K235R

    Promoter

    CMV

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pTET-IUPi

    Plasmid

    #122019

    Purpose

    Expresses IUP pathway genes (ChK, IPK, idi) under inducible promoter. aTC inducible. Kan. resist.

    Depositor

    Gregory Stephanopoulos

    Article

    Chatzivasileiou et al Proc Natl Acad Sci U S A. 2018 Dec 24. p

    Inserts

    Choline kinase (CKI1 Budding Yeast)
    Isopentenyl monophosphate kinase (AT1G26640 Mustard Weed)
    isopentenyl-diphosphate Delta-isomerase
    

    Expression

    Bacterial

    Mutation

    Codon optimized for E. coli

    Promoter

    pTET

    Available Since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pNMSB99

    Plasmid

    #199308

    Purpose

    MosSCI insertion of myo-3p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChR2-HDRC::SL2::jRGECO1a::let-858 3'UTR at ttTi5605

    Depositor

    Michael Krieg

    Article

    Porta-de-la-Riva et al Nat Methods. 2023 Apr 6. doi: 10.1038/s4

    Insert

    myo-3p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChR2-HDRC::SL2::jRGECO1a::let-858 3'UTR
    

    Expression

    Worm

    Mutation

    mTagBFP2 from pJJR81, ChR2(H134R;D156C), C. elega…

    Promoter

    myo-3

    Available Since

    Aug. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTIGER_mNeonGreen::3xFLAG::dCrk

    Plasmid

    #131137

    Purpose

    UASp construct for over-expression of Drosophila Crk with N-terminal monomeric NeonGreen and 3X FLAG tag. Compatible with PhiC31-mediated site-specific integration or classical P-element insertion.

    Depositor

    Mark Peifer

    Article

    Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

    Insert

    Crk (Crk Fly)
    

    Tags

    3XFLAG and mNeonGreen

    Expression

    Insect

    Available Since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBabe RFP-DN-FOXO1-HA neo

    Plasmid

    #45813

    Depositor

    Kevin Janes

    Article

    Wang et al Proc Natl Acad Sci U S A. 2011 Oct 4;108

    Insert

    FOXO1 (FOXO1 Human)
    

    Use

    Retroviral

    Tags

    HA and RFP

    Expression

    Mammalian

    Mutation

    aa1-255

    Available Since

    June 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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