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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 13,831 results for: sequence

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Showing: 7381 - 7400 of 13831 results

  6. hprtSAGFP

    Plasmid
    #41594
    Purpose
    In vivo single-strand annealing substrate composed of two truncated GFP genes, separated by a drug selection marker, and flanked by mouse hprt targeting sequences
    Depositor
    Maria Jasin
    Article
    Stark et al Mol Cell Biol. 2004 Nov;24(21):9305-16.
    Insert
    SA-GFP reporter
    Use
    Mouse Targeting; Dsb repair reporter substrate
    Expression
    Mammalian
    Available Since
    Dec. 3, 2012
    Availability
    Academic Institutions and Nonprofits only
    41594-plasmid-map-sequence-id-228244

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  14. pCASB

    Plasmid
    #190175
    Purpose
    Plasmid for yeast expression of Cas9; contains a golden gate cloning site to additionally express a gRNA for a target sequence
    Depositor
    Jessica Tyler
    Article
    Aguilar et al Methods Protoc. 2022 Oct 4;5(5):79. doi:
    Insert
    sgRNA cloning site
    Expression
    Bacterial and Yeast
    Mutation
    Added 5'-agagacc-3' upstream and 5'…
    Available Since
    Oct. 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    190175-plasmid-map-sequence-id-372326

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  15. p426MET25_SSKar2-sfpHluorin-HDEL (ER-sfpHluorin, MRV66)

    Plasmid
    #115698
    Purpose
    ER-targeted superfolder pHluorin for improved pH measurements in the ER of S. cerevisiae
    Depositor
    Eckhard Boles
    Article
    Reifenrath et al Sci Rep. 2018 Aug 10;8(1):11985. doi: 10
    Insert
    SSKar2-sfpHluorin-HDEL
    Tags
    ER retention signal containing HDEL and ER signal…
    Expression
    Yeast
    Mutation
    for introduced mutations see publication
    Promoter
    MET25
    Available Since
    Sept. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    115698-plasmid-map-sequence-id-223642

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  17. pT22-GluCNZ-6His

    Plasmid
    #213504
    Purpose
    Designed for cytoplasmic E.coli overexpression of glucanase (β-1,3-glycosidase) for gentle yeast spheroblasting that unlike Zymolyase prevents proteolysis of intracellular components.
    Depositor
    John Denu
    Article
    Kuznetsov et al J Biol Chem. 2018 Aug 3;293(31):12271-12
    Insert
    Glucanase (glucan endo-1,3-beta-glucosidase)
    Tags
    6His tag
    Expression
    Bacterial
    Mutation
    1-36 Signal Sequence Deletion
    Promoter
    T7
    Available Since
    March 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    213504-plasmid-map-sequence-id-424932

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  18. pMAL-c5x_UnaG

    Plasmid
    #163125
    Purpose
    A plasmid with UnaG coding sequence cloned into it. UnaG gene codon optimized for E. coli and B. theta. Expresses UnaG with N-term maltose-binding protein (MBP)-tag and C-term His-tag.
    Depositor
    Julie Biteen
    Article
    Chia et al Curr Res Microb Sci. 2020 Sep;1:1-6. doi
    Insert
    UnaG
    Tags
    His and MBP
    Expression
    Bacterial
    Available Since
    Feb. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    163125-plasmid-map-sequence-id-324911

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  19. EBOV 4cis

    Plasmid
    #249332
    Purpose
    Integration plasmid encoding EBOV essential replication factors (NP, VP35, VP30, and L protein)
    Depositor
    Amy Kistler
    Insert
    EBOV NP-P2A-VP35-P2A-VP30-P2A-L protein
    Use
    Piggybac donor vector
    Expression
    Bacterial and Mammalian
    Mutation
    the EBOV NP, VP35, and VP30 ORF sequences within …
    Promoter
    CAG
    Available Since
    Jan. 22, 2026
    Availability
    Academic Institutions and Nonprofits only
    249332-plasmid-map-sequence-id-497977

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  20. pENTR-R4R3-stop

    Plasmid
    #113745
    Purpose
    Contains three stop codons in every frame to ensure a premature stop codon when introduced into the genome via homology-directed repair. Gateway multisite entry clone.
    Depositor
    Magdalena Bezanilla
    Article
    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d
    Insert
    stop cassette
    Use
    CRISPR; Gateway entry vector
    Mutation
    Added 3 stop codons to the original sequence; see…
    Available Since
    Sept. 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    113745-plasmid-map-sequence-id-260896

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Showing: 7381 - 7400 of 13831 results