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FLM-L4132Δ

Plasmid

#41578

Depositor

Thomas Weimbs

Article

Talbot et al Proc Natl Acad Sci U S A. 2011 May 10;10

Insert

PKD1 (PKD1 Human)

Tags

CD16.7

Expression

Mammalian

Mutation

FLM L4132_

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
pDONR223-CDK3

Plasmid

#23368

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CDK3 (CDK3 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-XYLB

Plasmid

#23639

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

XYLB (XYLB Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-TESK2

Plasmid

#23695

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

TESK2 (TESK2 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-GALK2

Plasmid

#23748

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

GALK2 (GALK2 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pAG416GPD-Cerulean-YPT1

Plasmid

#18848

Depositor

Susan Lindquist

Article

Gitler et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

Insert

YPT1 (YPT1 Budding Yeast)

Tags

Cerulean

Expression

Yeast

Available Since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
pAG416GPD-Cerulean-YPT52

Plasmid

#18843

Depositor

Susan Lindquist

Article

Gitler et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

Insert

YPT52 (YPT52 Budding Yeast)

Tags

Cerulean

Expression

Yeast

Available Since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
pAG416GPD-Cerulean-YPT6

Plasmid

#18845

Depositor

Susan Lindquist

Article

Gitler et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

Insert

YPT6 (YPT6 Budding Yeast)

Tags

Cerulean

Expression

Yeast

Available Since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
HI.HslU.RSETB.fl.wt

Plasmid

#12556

Depositor

David McKay

Article

Trame et al Acta Crystallogr D Biol Crystallogr. 200

Insert

Haemophilus influenzae heat shock locus U

Expression

Bacterial

Mutation

none

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRpuro-sgROSA26

Plasmid

#231562

Purpose

pLentiCRISPR expression vector for Cas9 and gRNA targeting human ROSA26 locus (Puromycin selection marker)

Depositor

Rizwan Haq

Article

Schiantarelli et al Cancer Cell. 2025 Feb 10;43(2):308-316.e

Insert

sgROSA26

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6, EF-1a

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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AA331

Plasmid

#215954

Purpose

Fragmid fragment: (guide cassette) guide expression

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

U6_v1; BsmBI_v0; BsmBI_v1; trRNA_v2 [Sa]

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAtCas9-dead_3

Plasmid

#91167

Purpose

Cas9 only expression, Cas9 Type: AtCas9_D10A+H840A (dead), Plant Selection: 2x35S:bar

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9_D10A+H840A (dead)

Use

CRISPR

Expression

Plant

Mutation

D10A, H840A

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pMT1121

Plasmid

#8620

Depositor

Robert Hartley

Article

Hartley et al Protein Peptide Letters 1996 3:225-231

Insert

ribonuclease Sa, barstar

Expression

Bacterial

Available Since

Jan. 17, 2007

Availability

Academic Institutions and Nonprofits only

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pLV-EF1alpha-DEST-IRES-Puro

Plasmid

#253769

Purpose

Third generation lentiviral expression Gateway cloning destination vector under a human EF1alpha promoter for expression of a gene of interest linked to puromycin selection by an IRES.

Depositor

Samuel McBrayer

Article

Savani et al Nat Metab. 2026 Apr 29. doi: 10.1038/s42

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

May 1, 2026

Availability

Academic Institutions and Nonprofits only

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pT4-U6-sgRNA-CMV-EGFP

Plasmid

#239587

Purpose

Sleeping-beauty based EV for cloning and expression of sgRNAs - concomitant expression of GFP serves as transfection marker

Depositor

Andreas Moor

Article

Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

Type

Empty backbone

Use

Sleeping beauty transposone

Expression

Mammalian

Promoter

hU6

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pRCA360 - pBA904 Puro-T2A-GFP

Plasmid

#238164

Purpose

Lentiviral CRISPR guide vector expressing a non-targeting sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture

Depositor

Thomas Norman

Article

Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

Insert

Non-targeting sgRNA

Use

Lentiviral

Tags

GFP

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pUSE-URA3-Sec7-msYFP2

Plasmid

#218969

Purpose

Gene replacement plasmid to label S. cerevisiae Sec7 with msYFP2

Depositor

Benjamin Glick

Insert

SEC7-msYFP2 (SEC7 Budding Yeast)

Tags

msYFP2

Expression

Yeast

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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HRH3-DuET

Plasmid

#213314

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

HRH3 (HRH3 Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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pPBpuro-4493NES(Booster-PKA)

Plasmid

#184709

Purpose

PKA biosensor

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

Booster-PKA

Expression

Mammalian

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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OR52-consensus

Plasmid

#208080

Purpose

Consensus OR52 engineered from human OR52 family members

Depositor

Hiroaki Matsunami

Article

Ikegami et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

Insert

OR52-consensus

Tags

Rhodopsin tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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