We narrowed to 60,551 results for: Gene

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pT1QciMP

Plasmid

#62216

Purpose

QUAS transgenesis vector

Depositor

Harold Burgess

Article

Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

Type

Empty backbone

Use

Zebrafish transgenesis

Promoter

QUAS-E1b

Available Since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pKF257

Plasmid

#52533

Purpose

expresses I-SceI nuclease in Drosophila

Depositor

Klaus Foerstemann

Article

Bottcher et al Nucleic Acids Res. 2014 Apr 19.

Insert

I-SceI (SCEI Budding Yeast)

Tags

HA and NLS

Expression

Insect

Promoter

tubulin

Available Since

April 22, 2014

Availability

Academic Institutions and Nonprofits only

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MLM3583

Plasmid

#43887

Purpose

Activation Domain: VP64; RVD of C-terminal 0.5 TALE repeat: NG (binds T)

Depositor

Keith Joung

Article

Maeder et al Nat Methods. 2013 Feb 10. doi: 10.1038/n

Insert

TALE-activator VP64 Backbone

Use

Tale transcriptional activator expression vector

Promoter

EF1alpha

Available Since

March 8, 2013

Availability

Academic Institutions and Nonprofits only

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pNQL004-SOX2-FKBPV-HA2-P2A-mCherry targeting construct

Plasmid

#175552

Purpose

Targeting vector to introduce an FKBPV-HA2-P2A-mCherry cassette at the mouse SOX2 locus. Degradation Tag (dTAG) system.

Depositor

Elzo de Wit

Article

Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:

Insert

SOX2 (Sox2 Mouse)

Use

Mouse Targeting

Tags

FKBPV-HA2-P2A-mCherry

Expression

Mammalian

Mutation

none

Available Since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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P-Ef1a-RPL22-3XHA-P2A-mCherry-T2A-Puro

Plasmid

#170320

Purpose

Expresses HA and mCherry tagged RPL22, puromycin resistance

Depositor

Hemali Phatnani

Article

Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

Insert

RPL22 (RPL22 Human)

Use

Lentiviral

Mutation

None

Available Since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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P-Ef1a-RPL22-3XFlag-P2A-EGFP-T2A-Puro

Plasmid

#170318

Purpose

Expresses FLAG and EGFP tagged RPL22, puromycin resistance

Depositor

Hemali Phatnani

Article

Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

Insert

RPL22 (RPL22 Human)

Use

Lentiviral

Mutation

None

Available Since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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pT7-G-AsCas12a crRNA-BsaI cassette (MSP3491)

Plasmid

#114067

Purpose

T7 promoter crRNA entry vector used for in vitro transcription of AsCas12a guides

Depositor

Keith Joung, Benjamin Kleinstiver

Article

Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

Insert

entry vector for AsCas12a crRNAs

Use

In vitro transcription

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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AAV-tLuc-mCherry

Plasmid

#107553

Purpose

tLuc-mCherry in AAV backbone

Depositor

Juan Belmonte

Article

Liao et al Cell. 2017 Dec 14;171(7):1495-1507.e15.

Type

Empty backbone

Use

AAV

Available Since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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ACO2_pLX307

Plasmid

#98312

Purpose

Lentiviral expression of ACO2

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

ACO2 (ACO2 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pAOJ15

Plasmid

#91567

Purpose

Broad host range allelic exchange suicide vector for bacteria - Tse2 toxin driven by anhydrotetracycline inducible promoter

Depositor

Harry Mobley

Article

Armbruster et al PLoS Pathog. 2017 Jun 14;13(6):e1006434.

Type

Empty backbone

Expression

Bacterial

Promoter

Tetracycline promoter

Available Since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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pEMS1995

Plasmid

#49125

Purpose

AAV plasmid with GPR88 (Ple94) promoter driving expression of iCre.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-Ple94-iCre

Use

AAV

Expression

Mammalian

Promoter

GPR88

Available Since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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tdTomato/pTREX-b

Plasmid

#68709

Purpose

Expresses tdTomato in pTREX-b vector which confers resistance to blasticidin. This vector is used for cloning a specific sgRNA by BamHI, to transfect Cas9-expressing Trypanosoma cruzi epimastigotes.

Depositor

Roberto Docampo

Article

Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.

Insert

tdTomato

Use

Expression of tdtomato red fluorescence protein i…

Available Since

Oct. 8, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pMOTag4HM-crelox-HYG

Plasmid

#24029

Depositor

George Cross

Type

Empty backbone

Use

Cre/Lox

Tags

3XHA and 3XMyc

Available Since

Feb. 26, 2010

Availability

Academic Institutions and Nonprofits only

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pRA1EGFP

Plasmid

#193791

Purpose

Expresses EGFP under the control of PR promoter, pUC origin of replication, Ampicillin selection

Depositor

Sangram Bagh

Article

Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11

Insert

EGFP

Expression

Bacterial

Promoter

PR promoter

Available Since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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pUC Ad VAI

Plasmid

#175518

Purpose

For mammalian expression of adenovirus VAI RNA

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

Ad VAI RNA

Use

Adenoviral

Promoter

lac promoter

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pPTG-GG-sg2+sg3

Plasmid

#127199

Purpose

Plasmid expressing sgRNA2 (TACCACATTTGTAGAGGTT) & sgRNA3 (CAATGTATCTTATCATGTC) as polycistronic tRNA-gRNA from single U6 promoter

Depositor

Jordan Green

Article

Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

Insert

tRNA-gRNA

Use

Empty sgrna plasmid

Expression

Mammalian

Promoter

U6

Available Since

May 28, 2020

Availability

Academic Institutions and Nonprofits only

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pBABE (hygro) mEOS2 hYAP 1-2delta

Plasmid

#124162

Purpose

Protein expression for funtional studies of cell growth promotion

Depositor

Megan Finch-Edmondson, Marius Sudol

Article

Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

Insert

mEOS2-YAP1 (YAP1 Human)

Use

Unspecified

Mutation

None (Wild Type)

Available Since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 (-) hYAP 1-2beta

Plasmid

#124144

Purpose

Protein expression for funtional studies of cell growth promotion

Depositor

Megan Finch-Edmondson, Marius Sudol

Article

Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

Insert

YAP1 (YAP1 Human)

Use

Unspecified

Mutation

None (Wild Type)

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pCR8-CHD5-FRAGMENT

Plasmid

#68879

Purpose

GW entry vector for CHD5

Depositor

Adrian Bracken

Article

Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

Insert

CHD5 (CHD5 Human)

Use

Gateway Cloning

Mutation

331-672aa

Promoter

none

Available Since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only
pGEX-GST-CHD5(Chromos)-FRAG

Plasmid

#68875

Purpose

IPTG inducible bacterial expression of CHD5 fragment

Depositor

Adrian Bracken

Article

Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

Insert

CHD5 (CHD5 Human)

Tags

GST

Expression

Bacterial

Mutation

only the two chromodomains of CHD5

Promoter

tac

Available Since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only
pGGC_RT_XVE

Plasmid

#136970

Purpose

Goldengate/Greengate module for generation of beta-estradiole inducible genes.

Depositor

Klaus Theres

Insert

XVE

Expression

Bacterial

Mutation

BsaI site domestication 834 T > A,

Available Since

Jan. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pB6-Hipp11-td-sfGFP dre reporter-FNF-DTA

Plasmid

#139513

Purpose

B6J Hipp11-pCAG-rox-FNF-bGHpA-3xSV40pA-rox-td-sfGFP-WPRE-bpA allele targeting vector, low copy number

Depositor

Mario Capecchi

Insert

CAG rox stop rox td-sfGFP dre reporter

Use

Mouse Targeting; Dre/rox

Expression

Mammalian

Available Since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pCM305

Plasmid

#44550

Depositor

Michael Grunstein

Article

Millar et al Genes Dev. 2006 Mar 15;20(6):711-22.

Insert

HA-htz1

Expression

Yeast

Available Since

Oct. 23, 2013

Availability

Academic Institutions and Nonprofits only
pCM350

Plasmid

#44549

Depositor

Michael Grunstein

Article

Millar et al Genes Dev. 2006 Mar 15;20(6):711-22.

Insert

HA-htz1

Tags

HA

Expression

Yeast

Mutation

deleted amino acids 1-22

Available Since

May 21, 2013

Availability

Academic Institutions and Nonprofits only
6mSPL11F15R14::pBIB-BAS (6mSPL11)

Plasmid

#27387

Depositor

David Bartel

Article

Nodine et al Genes Dev. 2010 Dec 1. 24(23):2678-92.

Insert

SPL11 mutant (AT1G27360 Mustard Weed)

Use

Used to create transgenic lines

Mutation

miR156 target sites of SPL11 mutated

Available Since

May 11, 2011

Availability

Academic Institutions and Nonprofits only

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SPL11F15R14::pBIB-BAS (gSPL11)

Plasmid

#27398

Depositor

David Bartel

Article

Nodine et al Genes Dev. 2010 Dec 1. 24(23):2678-92.

Insert

SPL11 (AT1G27360 Mustard Weed)

Use

Used to create transgenic lines

Available Since

April 15, 2011

Availability

Academic Institutions and Nonprofits only

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pSPL10F13R13::pBIB-BAS (gSPL10)

Plasmid

#27407

Depositor

David Bartel

Article

Nodine et al Genes Dev. 2010 Dec 1. 24(23):2678-92.

Insert

SPL10 (AT1G27370 Mustard Weed)

Use

Used to create transgenic lines

Available Since

April 15, 2011

Availability

Academic Institutions and Nonprofits only

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6mpSPL10F13R13::pBIB-BAS (6mSPL10)

Plasmid

#27408

Depositor

David Bartel

Article

Nodine et al Genes Dev. 2010 Dec 1. 24(23):2678-92.

Insert

SPL10 mutant (AT1G27370 Mustard Weed)

Use

Used to create transgenic lines

Mutation

miR156 target sites of SPL10 mutated

Available Since

March 21, 2011

Availability

Academic Institutions and Nonprofits only

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SPL11p::pCGTAG (SPL11p::NLS-GFP)

Plasmid

#27411

Depositor

David Bartel

Article

Nodine et al Genes Dev. 2010 Dec 1. 24(23):2678-92.

Insert

SPL11 promoter (AT1G27360 Mustard Weed)

Mutation

SPL11 promoter transcriptionally fused to a nucle…

Available Since

Feb. 1, 2011

Availability

Academic Institutions and Nonprofits only

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UB13p::dsSPL10 v01 (SPL10-RNAi)

Plasmid

#27409

Depositor

David Bartel

Article

Nodine et al Genes Dev. 2010 Dec 1. 24(23):2678-92.

Insert

SPL10 inverted (AT1G27370 Mustard Weed)

Available Since

Jan. 20, 2011

Availability

Academic Institutions and Nonprofits only

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