We narrowed to 9,984 results for: EPO

1. PB-CAG-DDdCas9VP192-T2A-GFP-IRES-Neo

   Plasmid

   #102885

   Purpose

   PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   DDdCas9VP192-T2A-GFP-IRES-Neo
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   CAG

   Available Since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. Fireworks GFP[PTC-] (AVA2598)

   Plasmid

   #85445

   Purpose

   Expresses TEV protease and tandemly-repeated GFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a beta-globin reporter.

   Depositor

   Joan Steitz

   Article

   Alexandrov et al Mol Cell. 2017 Jan 5;65(1):191-201. doi:

   Insert

   tDNA1 EF1alfa-5xEGFP-TEVprotease-PEST-beta-globin(dI1)-BGH polyA tDNA2 FRT-PEST-TEVsite-PuromycinR
   

   Use

   Minimal backbone fragment for low-copy bacterial …

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-P(Per2)-DIO-mCherry-Venus-NLS-D2

   Plasmid

   #110058

   Purpose

   Per2 transcription fluorescence reporter

   Depositor

   Eric Erquan Zhang

   Article

   Mei et al Proc Natl Acad Sci U S A. 2018 Apr 2. pi

   Insert

   Per2 promoter and mCherry and Venus (Per2 Mouse)
   

   Use

   AAV and Cre/Lox

   Tags

   NLS-D2

   Expression

   Mammalian

   Mutation

   Promoter

   Per2

   Available Since

   May 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. TPI-PTC160

   Plasmid

   #130698

   Purpose

   Expresses TPI-PTC160 NMD reporter (It has a Premature termination codon (PTC) at 160th Amino acid)

   Depositor

   Marvin Tanenbaum

   Article

   Hoek et al Mol Cell. 2019 May 23. pii: S1097-2765(1

   Insert

   TPI-PTC160 (TPI1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   PTC160

   Promoter

   Pcmv

   Available Since

   Dec. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-ITR-PytR-mCherry

   Plasmid

   #204868

   Purpose

   Expresses pyrrolysyl tRNA and a wild-type mCherry reporter; can be packaged into AAV

   Depositor

   Abhishek Chatterjee

   Article

   Jewel et al Nat Methods. 2023 Jan;20(1):95-103. doi:

   Insert

   M. mazei pyrrolysyl tRNA
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   U25C

   Promoter

   U6

   Available Since

   Jan. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHR-DHFRY100I-sfGFP-NLS-P2A-NLS-mCherry-P2A_ ARHGAP5 5' and 3'UTR

   Plasmid

   #67931

   Purpose

   Translational reporter - ARHGAP5 UTRs

   Depositor

   Ron Vale

   Article

   Tanenbaum et al Elife. 2015 Aug 25;4. doi: 10.7554/eLife

   Insert

   sfGFP-NLS-P2A-NLS-mCherry (ARHGAP5 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. Fireworks RFP[PTC-] (AVA2515)

   Plasmid

   #85443

   Purpose

   Expresses TEV protease and tandemly-repeated RFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a beta-globin reporter.

   Depositor

   Joan Steitz

   Article

   Alexandrov et al Mol Cell. 2017 Jan 5;65(1):191-201. doi:

   Insert

   tDNA1 EF1alfa-5xtdTomato-TEVprotease-PEST-beta-globin(dI1)-BGH polyA tDNA2 FRT-HygromycinR
   

   Use

   Minimal backbone fragment for low-copy bacterial …

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pME V5-mTagBFP2 (JDW 830)

   Plasmid

   #224484

   Purpose

   Gateway compatible middle entry clone containing V5 tagged mTagBFP2 (Cytosolic blue fluorescent reporter)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   V5-mTagBFP2
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. ErbB-3-pGL3

   Plasmid

   #60899

   Purpose

   human ErbB-3 promoter reporter construct

   Depositor

   Frederick Domann

   Article

   Zhu et al Breast Cancer Res Treat. 2002 Jan;71(1):

   Insert

   ErbB-3 promoter (ERBB3 Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 19, 2014

   Availability

   Academic Institutions and Nonprofits only

10. Human NANOS3-P2A-mCherry knock-in targeting construct

    Plasmid

    #59721

    Purpose

    Gene targeting construct for human NANOS3 locus, introducing a P2A-mCherry reporter gene. Contains an frt-PGK-Neo-PA-frt cassette that can be excised.

    Depositor

    Jacob Hanna

    Article

    Irie et al Cell. 2015 Jan 15;160(1-2):253-68. doi:

    Insert

    P2A mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. QUAS-jGCaMP7s-T2A-tdTomato

    Plasmid

    #200010

    Purpose

    QUAS reporter line expressing GCaMP7s calcium indicator and tdTomato, separated by T2A

    Depositor

    Carolyn McBride

    Article

    Zhao et al Nature. 2022 May;605(7911):706-712. doi:

    Insert

    3xP3-ECFP-jGCaMP7s-td-Tomato
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    May 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. mKate2-P2A-APEX2-ML1N*2

    Plasmid

    #67665

    Purpose

    Mammalian expression vector driving APEX2-ML1N (Mouse 2xML1N domain, [marks PI(3,5)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    mKate2-P2A-APEX2-ML1N*2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair3

    Plasmid

    #98974

    Purpose

    Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 37bp 3' overhangs. TagBFP.

    Depositor

    Jan Karlseder

    Article

    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

    Insert

    LMNA sgRNAs pair 3 and Cas9(D10A)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV.CAG.Flex.Twitch2B.WPRE.SV40

    Plasmid

    #100039

    Purpose

    AAV expression of FLEXed Twitch2B driven by CAG promoter. Twitch 2B is a fluorescent reporter for calcium signaling

    Depositor

    Oliver Griesbeck

    Article

    Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

    Insert

    Twitch2B
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Jan. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. Twitch-3 pcDNA3

    Plasmid

    #49532

    Purpose

    Fluorescent reporter for calcium signaling

    Depositor

    Oliver Griesbeck

    Article

    Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

    Insert

    Twitch-3
    

    Use

    Tags

    ECFP and cpCit174

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV-GG-hUBC-EBFP2

    Plasmid

    #212314

    Purpose

    Lentiviral backbone with EBFP2 reporter

    Depositor

    Andrew Wilson

    Article

    Werder et al Sci Adv. 2022 Jul 15;8(28):eabo6566. doi

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV-miRFP680-NES-p2A-dTomato-2xrGBD

    Plasmid

    #222638

    Purpose

    Rho reporter

    Depositor

    Arnold Hayer

    Article

    Marshall-Burghardt et al Sci Adv. 2024 Sep 6;10(36):eadn6858. doi

    Insert

    miRFP680-NES-p2A-dTomato-2xrGBD
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTol2CG2-QUASM1:GFPNLS-SV40pA

    Plasmid

    #155123

    Purpose

    Tol2 vector containing QUASM1 reporter element upstream of GFP-NLS. Includes cmlc2:mRFP-SV40pA transgenesis marker

    Depositor

    Bret Pearson

    Article

    Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

    Insert

    QUASM1:GFPNLS
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    QUASM1-E1b

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET29a-hNABP2

    Plasmid

    #128307

    Purpose

    Express hNABP2 (a.k.a hSSB1 or SOSSB1) in E. coli with a C-terminal His-tag

    Depositor

    Yuliang Wu

    Article

    Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

    Insert

    Human Nucleic Acid Binding Protein 2 (hNABP2) (NABP2 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Y85C- please see depositor comments below

    Promoter

    T7

    Available Since

    Aug. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRK5-FLAG-Sestrin1 isoform1

    Plasmid

    #72594

    Purpose

    overexpression, codon optimized for mammalian expression

    Depositor

    David Sabatini

    Article

    Chantranupong et al Cell Rep. 2014 Oct 9;9(1):1-8. doi: 10.1

    Insert

    SESN1 (SESN1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    codon optimized for mammalian expression, see dep…

    Promoter

    CMV

    Available Since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only