We narrowed to 8,708 results for: sgRNA

1. SlutrCas9

   Plasmid

   #163795

   Purpose

   Expresses SlutrCas9, and cloning backbone for sgRNA

   Depositor

   Yongming Wang

   Article

   Hu et al Nucleic Acids Res. 2021 Mar 15. pii: 617

   Type

   Empty backbone

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. PiggyBac EF1a-eGFP-Puro U6 PPP2CA g2

   Plasmid

   #170823

   Purpose

   PiggyBac Cas13d sgRNA plasmid for PPP2CA knockdown

   Depositor

   Xiaoping Bao

   Article

   Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

   Insert

   Cas13d PPP2CA gRNA2
   

   Use

   Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. Cas12f-GE ver4.1

   Plasmid

   #176544

   Purpose

   Expresses Cas12f-GE in mammalian cells

   Depositor

   Yong-Sam Kim

   Article

   Kim et al Nat Biotechnol. 2021 Sep 2. pii: 10.1038

   Inserts

   Cas12f-GE ver4.1
   Cas12f-GE ver4.1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and chicken β-actin

   Available since

   Oct. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSJX022

   Plasmid

   #232325

   Purpose

   Genome editing in Caulobacter crescentus

   Depositor

   Wei Zhao

   Article

   Sun et al bioRxiv 2024.12.02.626314

   Inserts

   sgRNA
   SpCas9M
   

   Use

   CRISPR

   Tags

   H-arms and cat

   Expression

   Bacterial

   Mutation

   Promoter

   PJ23119 and Pvan

   Available since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSJX024

   Plasmid

   #232327

   Purpose

   Genome editing in Caulobacter crescentus

   Depositor

   Wei Zhao

   Article

   Sun et al bioRxiv 2024.12.02.626314

   Inserts

   sgRNA
   SpCas9M
   

   Use

   CRISPR

   Tags

   H-arms and cat

   Expression

   Bacterial

   Mutation

   Promoter

   PJ23119 and Pxyl

   Available since

   June 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSJX016

   Plasmid

   #232324

   Purpose

   Genome editing in Caulobacter crescentus

   Depositor

   Wei Zhao

   Article

   Sun et al bioRxiv 2024.12.02.626314

   Inserts

   sgRNA
   SpCas9M
   

   Use

   CRISPR

   Tags

   H-arms and sfGFP

   Expression

   Bacterial

   Mutation

   Promoter

   PJ23119 and Pvan

   Available since

   June 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJMP1339

   Plasmid

   #119271

   Purpose

   for cloning new sgRNAs

   Depositor

   Oren Rosenberg, Carol Gross, Jason Peters

   Article

   Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

   Insert

   dcas9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D10A and H840A

   Promoter

   Available since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. sgTrack-BFP

   Plasmid

   #164273

   Purpose

   Empty sgRNA expression vector with co-expression of BFP reporter

   Depositor

   Charles Rudin

   Article

   Wohlhieter et al Cell Rep. 2020 Dec 1;33(9):108444. doi:

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and EFS

   Available since

   Feb. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLentiCRISPRv2 sgIRF3#5

   Plasmid

   #127642

   Purpose

   Knock-out of human IRF3

   Depositor

   Nicolas Manel

   Article

   Gratia et al J Exp Med. 2019 May 6;216(5):1199-1213.

   Insert

   IRF3 sgRNA (IRF3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPMQAK1-Ptrc-[E*](104)-Cas9-B0015-lacZ-PnrsB-mazF-LVA

    Plasmid

    #190790

    Purpose

    [E*](104) construct. Theophylline inducible Cas9 (S. pyogenes) expression. Two BsaI-sites allow for simultaneous insertion of sgRNA and donor DNA. Includes a nickel-inducible curing system.

    Depositor

    Paul Hudson

    Article

    Cengic et al ACS Synth Biol. 2022 Aug 15. doi: 10.102

    Inserts

    Cas9
    mazF-LVA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    LVA

    Expression

    Bacterial

    Mutation

    silent mutations of BpiI-sites in original Cas9 g…

    Promoter

    PnrsB and Ptrc-[E*](104)

    Available since

    Feb. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJMP1337

    Plasmid

    #119270

    Purpose

    for cloning new sgRNAs

    Depositor

    Oren Rosenberg, Carol Gross, Jason Peters

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    dcas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJEP10-AAV-U6/TO-gRNA(Empty)-CMV-TetR-P2A-eGFP-KASH-pA

    Plasmid

    #82706

    Purpose

    AAV backbone with a full length U6/TO promoter driving expression of a sgRNA. The gRNA can be inserted via Sapl sites. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.

    Depositor

    Jonathan Ploski

    Article

    de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do

    Insert

    U6/TO Empty gRNA Expression Cassette
    

    Use

    AAV and CRISPR

    Tags

    CMV promoter driving expression of TetR-P2A-GFP-K…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX458-DsRed2

    Plasmid

    #112219

    Purpose

    Cloning backbone for sgRNA, co-expresses human optimized S. pyogenes Cas9 and DsRed2

    Depositor

    Yinming Liang

    Article

    Luo et al J Biotechnol. 2018 Jun 7;281:11-20. doi:

    Insert

    DsRed2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    DsRed2 contains a silent nucleotide substitution …

    Promoter

    Available since

    July 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. TLCV2-RB1

    Plasmid

    #87836

    Purpose

    LentiCRISPR v2 was modified into an all-in-one dox inducible system. The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter drives constitutive expression of an sgRNA targeting human RB1.

    Depositor

    Adam Karpf

    Article

    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

    Insert

    sgRB1 (RB1 Human)
    

    Use

    CRISPR and Lentiviral; Doxycycline inducible; egf…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tight TRE promoter and U6

    Available since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. scaffold-linker-U6

    Plasmid

    #89639

    Purpose

    construction of paired sgRNAs driven by two U6 promoters. Vector itself only has one U6 promoter.

    Depositor

    Wensheng Wei

    Article

    Zhu et al Nat Biotechnol. 2016 Dec;34(12):1279-128

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    June 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSNR52-sgTEF1

    Plasmid

    #46922

    Purpose

    Yeast CEN/ARS vector (Ura3) that contains sgRNA controlled by SNR 52 promoter, targeting endogenous TEF1 promoter

    Depositor

    Jonathan Weissman, Stanley Qi

    Article

    Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

    Insert

    sgTEF1 promoter (TEF1 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available since

    Oct. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEJS2601_AAV-Nme2-ABE8e-i1-U6-2xSapI

    Plasmid

    #201683

    Purpose

    All-in-one AAV plasmid expressing Nme2-ABE8e-i1 and U6 sgRNA cassette. SapI enables cloning new spacers.

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

    Insert

    Nme2-ABE8e-i1
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U1a

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJMP1356

    Plasmid

    #119276

    Purpose

    for cloning new sgRNAs

    Depositor

    Oren Rosenberg, Carol Gross, Jason Peters

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    dcas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX330 PLIN2

    Plasmid

    #202196

    Purpose

    pX330 backbone expressing sgRNA to edit the C-terminus of human PLIN2

    Depositor

    James Olzmann

    Article

    Roberts et al Dev Cell. 2023 Jul 18:S1534-5807(23)0033

    Insert

    PLIN2 (PLIN2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. LentiCRISPRv2Cre

    Plasmid

    #82415

    Purpose

    Lentiviral vector expressing Cre recombinase alongside Cas9 and an sgRNA cloning site

    Depositor

    David Feldser

    Article

    Walter et al Cancer Res. 2017 Feb 15. pii: canres.215

    Insert

    Cre Recombinase
    

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    Expression

    Mutation

    Mutated BsmBI cut site

    Promoter

    EFS (P2A)

    Available since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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