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mEGFP-2xbiRhoBAST

Plasmid

#196349

Purpose

Expression of mEGFP mRNA with 2xbiRhoBAST in 3'UTR

Depositor

Murat Sunbul

Article

Buhler et al Nat Chem Biol. 2023 Apr;19(4):478-487. d

Insert

mEGFP-2xbiRhoBAST

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pTol2-Huc-Positron-ST

Plasmid

#129265

Purpose

pan neuronal expression of soma-localized Positron in zebrafish

Depositor

Eric Schreiter

Article

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

Positron-ST

Use

Zebrafish expression

Mutation

D92N, E199V

Available Since

Aug. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-luxOFP

Plasmid

#229950

Purpose

Mammalian expression of a de novo designed, FRET-based neoLux1.2-CyOFP1 fusion bioluminescent probe (max. Em=588)

Depositor

Andy Yeh

Article

Chen et al Chem. 2025 Mar 13;11(3):102346. doi: 10.

Insert

luxOFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pALPS puro miR30-IRF9

Plasmid

#117159

Purpose

Target site AATTATCACAAAGAGGACAGG

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

shRNA targeting miR30

Use

Lentiviral

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-VCre

Plasmid

#89575

Purpose

Expresses VCre under pCAG

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

VCre recombinase

Use

Cre/Lox and Synthetic Biology

Tags

NLS

Expression

Mammalian

Available Since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pALPS puro miR30-IRF5

Plasmid

#117157

Purpose

Target site TATTTCCCTGTCTCCTTGGCC

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

shRNA targeting miR30

Use

Lentiviral

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pALPS puro miR30-IRF7

Plasmid

#117158

Purpose

Target site ATAAGGAAGCACTCGATGTCG

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

shRNA targeting miR30

Use

Lentiviral

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pAM420 GFP-titin-ssrA substrate

Plasmid

#226362

Purpose

Expression of GFP-titin-ssrA substrate for 26S proteasome

Depositor

Andreas Martin

Article

Bashore et al Nat Struct Mol Biol. 2015 Sep;22(9):712-

Insert

GFP-titin substrate

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-luxKate

Plasmid

#229951

Purpose

Mammalian expression of a de novo designed, FRET-based neoLux1.2-mKate2 fusion bioluminescent probe (max. Em=638)

Depositor

Andy Yeh

Article

Chen et al Chem. 2025 Mar 13;11(3):102346. doi: 10.

Insert

luxKate

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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RV-mCD4-HH5-1

Plasmid

#124961

Purpose

Express the Helicobacter hepaticus specific TCRa/b HH5-1 in T cell hybridoma cells

Depositor

Dan Littman

Article

Xu et al Nature. 2018 Feb 15;554(7692):373-377. d

Insert

HH5-1

Use

retroviral

Expression

Mammalian

Available Since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

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NES-Halo

Plasmid

#200201

Purpose

Expression of 3X HIV NES

Depositor

Sheila Teves

Article

Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

Insert

3X HIV Rev NES-Halo

Tags

Halo

Expression

Mammalian

Mutation

None

Promoter

EF_1alpha

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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RV-mCD4-HH5-5

Plasmid

#124985

Purpose

Express the Helicobacter hepaticus specific TCRa/b HH5-5 in T cell hybridoma cells

Depositor

Dan Littman

Article

Xu et al Nature. 2018 Feb 15;554(7692):373-377. d

Insert

HH5-5 TCRa P2A TCRb

Use

retroviral

Expression

Mammalian

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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RV-mCD4-HH5-4

Plasmid

#124984

Purpose

Express the Helicobacter hepaticus specific TCRa/b HH5-4 in T cell hybridoma cells

Depositor

Dan Littman

Article

Xu et al Nature. 2018 Feb 15;554(7692):373-377. d

Insert

HH5-4 TCRa P2A TCRb

Use

retroviral

Expression

Mammalian

Available Since

June 7, 2019

Availability

Academic Institutions and Nonprofits only

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pMJ824

Plasmid

#39314

Depositor

Jennifer Doudna

Article

Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

Insert

Cas9

Use

CRISPR

Tags

GST and His6

Expression

Bacterial

Promoter

T7

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pGEX-5X1 Flag PA1

Plasmid

#15560

Depositor

Kai Ge

Article

Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

Insert

PA1 (PAGR1 Human)

Tags

Flag and GST

Expression

Bacterial

Available Since

Aug. 16, 2007

Availability

Academic Institutions and Nonprofits only
pNL4-3 envFS eGFP gag2xFS

Plasmid

#101341

Purpose

1st frameshift is CG nucleotide insertion in MA at nt 832. 2nd is a CTAG addition in CA at nt 1508.

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

NL4-3

Use

Lentiviral

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1 Stu2 1-306

Plasmid

#38315

Depositor

Anthony Hyman

Article

Widlund et al Mol Biol Cell. 2012 Sep 19.

Insert

5' 918 bp from STU2 ORF (STU2 Budding Yeast)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pREDusk-StrR-MCS

Plasmid

#188972

Purpose

For red light-repressed bacterial expression of gene of interest (StrR)

Depositor

Heikki Takala

Article

Multamaki et al ACS Synth Biol. 2022 Aug 23. doi: 10.102

Type

Empty backbone

Expression

Bacterial

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-Ace2_D92N_E199V-mNeon

Plasmid

#129256

Purpose

Express Ace2_D92N_E199V-mNeon in mammalian cells

Depositor

Eric Schreiter

Article

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

Ace2_D92N_E199V-mNeon

Expression

Mammalian

Mutation

D92N, E199V

Promoter

CAG

Available Since

Oct. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pUAS-Cre

Plasmid

#50797

Purpose

Expression of Cre recombinase under the control of the UAS-promoter

Depositor

Connie Cepko

Article

Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

Insert

Cre recombinase

Use

Multi-species

Promoter

UAS-hsp70

Available Since

March 20, 2014

Availability

Academic Institutions and Nonprofits only

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