We narrowed to 8,707 results for: ALP

1. pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry WT

   Plasmid

   #163991

   Purpose

   Mammalian expression vector encoding codon-optimized human NLRP1 CARD with a C-terminal mCherry tag for imaging

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   NLRP1 CARD (NLRP1 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Available Since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYI2165

   Plasmid

   #228347

   Purpose

   2,4-Diacetylphloroglucinol (DAPG)-regulatable ALX-0171 expression

   Depositor

   Jun Ishii

   Article

   Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

   Insert

   MFalpha(2xAdv)-LE-ALX-0171-3A-FLAG-His
   

   Use

   Affinity Reagent/ Antibody

   Tags

   AAA linker followed by FLAG-His6 and MF_ secretio…

   Expression

   Yeast

   Promoter

   2,4-Diacetylphloroglucinol-regulatable synthetic …

   Available Since

   March 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGB 35s:dCas:BRD:tNos (GB1172)

   Plasmid

   #75401

   Purpose

   Transcriptional unit of (human codon optimized) inactivated Cas9 fused to the BRD Transcriptional Repressor

   Depositor

   Diego Orzaez

   Article

   Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

   Insert

   dCas9:BRD
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Promoter

   35S

   Available Since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLX209-neo-HA-Ub-T2A-Tet3G

   Plasmid

   #202436

   Purpose

   Bicistronic expression of HA-tagged Ubiquitin (HA-Ubiquitin) and Tet-On 3G transactivator (Tet3G)

   Depositor

   William Hahn

   Article

   Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

   Insert

   Ubiquitin
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF1alpha

   Available Since

   June 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-Sac-Abeta(MC1-42)

   Plasmid

   #127151

   Purpose

   Expresses N-terminal cysteine Abeta(1-42)

   Depositor

   James Nowick

   Article

   Zhang et al Biochemistry. 2021 Apr 20;60(15):1191-12

   Insert

   Abeta(MC1-42) (APP Human)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHR-RUNX2(+10A)-IDR-mCherry-CRY2-NLS

   Plasmid

   #145268

   Purpose

   Mammalian Expression of Nucelar Opto RUNX2(+10A) IDR with SV40 NLS

   Depositor

   Denes Hnisz

   Article

   Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

   Insert

   RUNX2(+10A)-IDR (RUNX2 Human)
   

   Expression

   Mammalian

   Mutation

   A-repeat expanded

   Available Since

   Sept. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHR_Gal4UAS_4D5-Low_CAR-mCherry_pGK_4D5-Low_SynNotch_RHL183

   Plasmid

   #164827

   Purpose

   Lentiviral vector for Gal4 inducible AntiHER2 4D5-Low CAR-mCherry and constitutive expression of antiHER2 4D5-Low Gal4VP64 Synthetic Notch

   Depositor

   Wendell Lim

   Article

   Hernandez-Lopez et al Science. 2021 Mar 12;371(6534):1166-1171

   Inserts

   AntiHER2 4D5-Low Chimeric Antigen receptor (CD8alphaTM-41BB-CD3z)
   AntiHER2 4D5-Low Gal4VP64 synthetic Notch receptor
   

   Use

   Lentiviral

   Tags

   mCherry

   Promoter

   miniCMV and pGK

   Available Since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHR_Gal4UAS_4D5-Low_CAR-mCherry_pGK_4D5-Medium_SynNotch_RHL144

   Plasmid

   #164828

   Purpose

   Lentiviral vector for Gal4 inducible AntiHER2 4D5-Low CAR-mCherry and constitutive expression of antiHER2 4D5-Medium Gal4VP64 Synthetic Notch

   Depositor

   Wendell Lim

   Article

   Hernandez-Lopez et al Science. 2021 Mar 12;371(6534):1166-1171

   Inserts

   AntiHER2 4D5-Low Chimeric Antigen receptor (CD8alphaTM-41BB-CD3z)
   AntiHER2 4D5-Medium Gal4VP64 synthetic Notch receptor
   

   Use

   Lentiviral

   Tags

   mCherry

   Promoter

   miniCMV and pGK

   Available Since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. qTAG-N-Puro-moxGFP-TUBA1B

   Plasmid

   #207767

   Purpose

   Donor template for Puro-2A-moxGFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   TUBA1B Homology Arms flanking a Puro-moxGFP Cassette (TUBA1B Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pENTR221-EF1.Pro

    Plasmid

    #79488

    Purpose

    MultiSite Gateway entry clones, first fragment, (attL1, attR5) Human EF1 promoter

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    EF1 Promoter (EEF1A1 Human, Synthetic)
    

    Use

    Multisite gateway entry clone

    Promoter

    EF1 Promoter

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHH0103_EEF1A1_GTP-EFTU

    Plasmid

    #109861

    Purpose

    Protein expression and purification of EEF1A1_GTP-EFTU

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    EEF1A1_GTP-EFTU (EEF1A1 Human)
    

    Expression

    Bacterial

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA-GFP-EphrinB3-Flag

    Plasmid

    #155012

    Purpose

    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EphrinB3 from pcDNA3.1

    Depositor

    Sinisa Urban

    Article

    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

    Insert

    EphrinB3 (EPHB3 Human)
    

    Tags

    Flag, GFP, and signal peptide (residues 1 to 32) …

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. TFORF1916

    Plasmid

    #144317

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    RUNX2 (RUNX2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSpCas9(BB)-2A-Puro(PX459)-GJA1 sgRNA

    Plasmid

    #164471

    Purpose

    Expresses sgRNA targeting human GJA1 locus

    Depositor

    Markus Kuehn

    Article

    Cheng et al Stem Cells. 2022 Mar 9. pii: 6545973. do

    Insert

    Gap junction alpha-1 (GJA1) (GJA1 Human)
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCSIIpuro-mNG-eDHFR(69K6)-RasGEF

    Plasmid

    #172104

    Purpose

    Mammalian expression of RasGRF1(Cdc25) fused to mNeonGreen-eDHFR(69K6)

    Depositor

    Shinya Tsukiji

    Article

    Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

    Insert

    mNeonGreen-eDHFR(69K6)-RasGRF1(Cdc25) (RASGRF1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1-alpha

    Available Since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHRIG-AktDN

    Plasmid

    #53597

    Purpose

    lentiviral expression of dominant negative mouse Akt1 and EGFP

    Depositor

    Heng Zhao

    Article

    Xie et al J Cereb Blood Flow Metab. 2013 Dec;33(12

    Insert

    Akt1 (Akt1 Mouse)
    

    Use

    Lentiviral

    Tags

    HA and IRES-EGFP

    Expression

    Mammalian

    Mutation

    Dominant negative (K179M)

    Promoter

    CMV

    Available Since

    Aug. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

17. Ef1a-DIO-BiPOLES-mCerulean

    Plasmid

    #154949

    Purpose

    Optogenetic tool for blue-light inhibition and red-light excitation of neurons

    Depositor

    Simon Wiegert

    Article

    Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

    Insert

    BiPOLES
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Ef1-alpha

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRRLNeo-pEF1a-p53ash-mCerulean

    Plasmid

    #69579

    Purpose

    Expresses p53 tagged with mCerulean

    Depositor

    Galit Lahav

    Article

    Gaglia et al Proc Natl Acad Sci U S A. 2013 Sep 17;11

    Insert

    p53 (TP53 Human)
    

    Use

    Lentiviral

    Tags

    mCerulean

    Expression

    Mammalian

    Mutation

    7 silent mutations in p53 DNA to prevent targetin…

    Promoter

    EF1alpha

    Available Since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDH-EF1-FHC-POLQ-K121M

    Plasmid

    #64877

    Purpose

    lentiviral expression of human POLQ K121M mutant (a mutation in the conserved ATP-binding site of the Walker A motif in the helicase-like domain)

    Depositor

    Richard Wood

    Article

    Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.

    Insert

    POLQ (POLQ Human)
    

    Use

    Lentiviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    K121M, a mutation in the conserved ATP-binding si…

    Promoter

    EF1alpha

    Available Since

    Oct. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pENTR-myr-AKT-HA

    Plasmid

    #31790

    Depositor

    Xin Chen

    Article

    Calvisi et al Gastroenterology. 2011 Mar;140(3):1071-8

    Insert

    Akt1 (Akt1 Mouse)
    

    Use

    Entry vector

    Tags

    HA and Myr

    Available Since

    Oct. 21, 2011

    Availability

    Academic Institutions and Nonprofits only