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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,918 results for: EGF

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Showing: 7501 - 7520 of 14918 results

  8. pCMV-T7-BPNLS-ABE8e-iSpyMac-BPNLS-P2A-EGFP (LLH528)

    Plasmid
    #208289
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with iSpyMac(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    pCMV-T7-BPNLS-ABE8e-iSpyMac-BPNLS-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8e mutations in TadA; mutations in iSpyMac
    Promoter
    CMV and T7
    Available Since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    208289-plasmid-map-sequence-id-410849

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  9. EK0409 SFFV-mCherry-SGc(s70587)cSG-EGFP (FLP-IN)

    Plasmid
    #191164
    Purpose
    Similar to EK0285 but has additional restriction sites around the sensor sequence. Recommended for cloning new sensors when the presence of MS2 sequences are not relevant (use EK0438 if MS2 sequences
    Depositor
    Xiaojing Gao
    Article
    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038
    Insert
    mCherry:s70587:EGFP (additional cutsites)
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    SFFV
    Available Since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    191164-plasmid-map-sequence-id-375473

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  10. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide1

    Plasmid
    #118158
    Purpose
    CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA1 against the EGFP CDS
    Depositor
    Jorge Ferrer
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Promoter
    EF1a core and U6
    Available Since
    April 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118158-plasmid-map-sequence-id-240404

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  11. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR EGFP-guide1

    Plasmid
    #118162
    Purpose
    CRISPRi negative control. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter, and sgRNA1 against the EGFP CDS
    Depositor
    Jorge Ferrer
    Article
    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Promoter
    hPGK and U6
    Available Since
    April 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118162-plasmid-map-sequence-id-240427

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  12. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR EGFP-guide2

    Plasmid
    #118163
    Purpose
    CRISPRi negative control. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter, and sgRNA2 against the EGFP CDS
    Depositor
    Jorge Ferrer
    Article
    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Promoter
    hPGK and U6
    Available Since
    April 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118163-plasmid-map-sequence-id-240400

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  14. pCMV-T7-BPNLS-CE-ABE8e-SpRY--BPNLS-P2A-EGFP (NK289)

    Plasmid
    #208293
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized CE-ABE8e-SpRY A-to-G base editor and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    pCMV-T7-BPNLS-CE-ABE8e-SpRY-BPNLS-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8e mutations in TadA inlaid into nSpRY(D10A/A6…
    Promoter
    CMV and T7
    Available Since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    208293-plasmid-map-sequence-id-410845

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  15. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR EGFP-guide3

    Plasmid
    #118164
    Purpose
    CRISPRi negative control. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter, and sgRNA3 against the EGFP CDS
    Depositor
    Jorge Ferrer
    Article
    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Promoter
    hPGK and U6
    Available Since
    April 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118164-plasmid-map-sequence-id-240433

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Showing: 7501 - 7520 of 14918 results