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Showing: 7501 - 7520 of 19678 results

  2. pET28a-mOct4 Full length(FL)

    Plasmid
    #206394
    Purpose
    Expresses Oct4 with a 6xHis tag for protein purification
    Depositor
    Ralf Jauch
    Insert
    Oct4 (Pou5f1 Mouse)
    Tags
    6xHis
    Expression
    Bacterial
    Available since
    Oct. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    206394-plasmid-map-sequence-id-409984

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  3. pTS116 pHAGE2 CMVtetO2 MCS-TEV-SUMOstar-22xGS-Alfa-P2A-TagBFP

    Plasmid
    #199369
    Purpose
    Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-SUMOstar-ALFA-P2A-BFP-tagged protein in mammalian cells.
    Depositor
    Rebecca Voorhees
    Publication
    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    TEV-SUMOstar-ALFA-P2A-TagBFP
    Expression
    Mammalian
    Mutation
    WT
    Available since
    April 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    199369-plasmid-map-sequence-id-389901

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  6. pcDNA TfR1-miniE

    Plasmid
    #181987
    Purpose
    MiniE genetically encoded tag on Transferrin Receptor 1 (TfR1) to be used with MiniR labeled with a reporter
    Depositor
    Kimberly Beatty
    Publication
    Doh et al Biochemistry. 2020 Aug 25;59(33):3051-30
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TfR1-MiniE
    Expression
    Mammalian
    Available since
    March 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    181987-plasmid-map-sequence-id-377037

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  7. AAVS1_Puro_hPGK1_mScarlet-I-TOSI_Donor

    Plasmid
    #178087
    Purpose
    HDR donor plasmid to introduce the mScarlet-I mTOR signaling indicator (TOSI) cassette to AAVS1 using AAVS1 T2 sgRNA.
    Depositor
    Yannick Doyon
    Publication
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mScarlet-I mTOR signaling indicator (TOSI)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    Human PGK1
    Available since
    Dec. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    178087-plasmid-map-sequence-id-350132

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  10. pLKO_HA.NWS.VSVg_NGFR

    Plasmid
    #158243
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Publication
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    HA.NWS.VSVg
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158243-plasmid-map-sequence-id-329479

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  11. pLKO_HA.FLAG.VSVg_NGFR

    Plasmid
    #158245
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Publication
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    HA.FLAG.VSVg
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158245-plasmid-map-sequence-id-327706

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  12. pSaPIPLC His

    Plasmid
    #173810
    Purpose
    Expression and purification of mature, His-tagged Staphylococcus aureus (strain FPR3757) phosphaatidyl inositol specific phospholipase C (PI-PLC) from E. coli BL21 CodonPlus (DE3) RIL cells
    Depositor
    Anne Gershenson, Mary F Roberts
    Publication
    Goldstein et al Biochemistry. 2012 Mar 27;51(12):2579-87
    Insert
    Staphylococcus aureus plc
    Tags
    6XHis
    Expression
    Bacterial
    Mutation
    none
    Promoter
    T7
    Available since
    Aug. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    173810-plasmid-map-sequence-id-341162

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  13. sgCTNNB1 Nicking

    Plasmid
    #169845
    Purpose
    Express a nicking sgRNA used for installation of the oncogenic S45F or S45del in Ctnnb1 in mouse liver.
    Depositor
    Wen Xue
    Publication
    Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgCTNNB1 Nicking (Ctnnb1 Mouse)
    Expression
    Mammalian
    Available since
    June 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    169845-plasmid-map-sequence-id-334033

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  14. pegCTNNB1 S45del

    Plasmid
    #169844
    Purpose
    pegRNA used for installation (via TCC deletion) of the oncogenic S45del in Ctnnb1 in mouse liver.
    Depositor
    Wen Xue
    Publication
    Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    pegCTNNB1 S45DEL (Ctnnb1 Mouse)
    Expression
    Mammalian
    Available since
    June 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    169844-plasmid-map-sequence-id-334036

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  15. pegCTNNB1 S45F

    Plasmid
    #169843
    Purpose
    pegRNA used for installation (via C•G-to-T•A) of the oncogenic S45F in Ctnnb1 in mouse liver.
    Depositor
    Wen Xue
    Publication
    Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    pegCTNNB1 S45F (Ctnnb1 Mouse)
    Expression
    Mammalian
    Available since
    June 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    169843-plasmid-map-sequence-id-334034

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  17. pGBW-m4046647

    Plasmid
    #153940
    Purpose
    Yeast expression plasmid for SARS-CoV-2 3C-like proteinase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Expression
    Yeast
    Mutation
    wt
    Promoter
    pGAL1
    Available since
    June 12, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153940-plasmid-map-sequence-id-299997

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  19. FB007

    Plasmid
    #119705
    Purpose
    Promoter gpdA from Aspergillus nidulans domesticated into pUPD2 with GGAG/AATG barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformation.
    Depositor
    Jose Marcos
    Publication
    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d
    Insert
    Promoter gpdA
    Use
    Synthetic Biology; Domestication of dna parts for…
    Promoter
    gpdA
    Available since
    March 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    119705-plasmid-map-sequence-id-237147

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Showing: 7501 - 7520 of 19678 results