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EF1a-mRuby2(miRE.FF4)-TS.FF6x4-bGH

Plasmid

#235293

Purpose

ComMAND open-loop circuit regulating mRuby2 with 4 copies of microRNA target sites

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

June 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a-TS.FF6x1-mRuby2(miR.FF5)-bGH

Plasmid

#235278

Purpose

ComMAND open-loop circuit regulating mRuby2 with miR-FF5 design 2

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a-TS.FF5x1-mRuby2-miR.FF5-bGH

Plasmid

#235281

Purpose

ComMAND closed-loop circuit regulating mRuby2 with miR-FF5 design 3

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
EFS-mRuby2(miRE.FF4)-TS.FF6x1-bGH

Plasmid

#235309

Purpose

ComMAND open-loop circuit regulating mRuby2, EFS-driven

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EFS (human EF1a short, intronless)

Available Since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a-TS.FF6x1-mRuby2-miR.FF5-bGH

Plasmid

#235280

Purpose

ComMAND open-loop circuit regulating mRuby2 with miR-FF5 design 3

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a-TS.FF5x1-mRuby2(miR.FF5)-bGH

Plasmid

#235279

Purpose

ComMAND closed-loop circuit regulating mRuby2 with miR-FF5 design 2

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
hPGK-mRuby2(miRE.FF4)-TS.FF6x1-bGH

Plasmid

#235311

Purpose

ComMAND open-loop circuit regulating mRuby2, hPGK-driven

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

hPGK (human phosphoglycerate kinase)

Available Since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
hPGK-mRuby2(miRE.FF4)-TS.FF4x1-bGH

Plasmid

#235312

Purpose

ComMAND closed-loop circuit regulating mRuby2, hPGK-driven

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

hPGK (human phosphoglycerate kinase)

Available Since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
CAG-mRuby2(miRE.FF4)-TS.FF4x1-bGH

Plasmid

#235298

Purpose

ComMAND closed-loop circuit regulating mRuby2, CAG-driven

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

CAG (synthetic cytomegalovirus early enhancer + c…

Available Since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pPB-[EF1a-mRuby2-bGH]-UbC-PuroR-T2A-mGL-SV40pA

Plasmid

#235325

Purpose

PiggyBac integration vector for ComMAND base gene regulating mRuby2

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology; Piggybac

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST-Dok1-mScarlet

Plasmid

#215535

Purpose

Expression vector with Dok1 tagged with C-terminal mScarlet

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

DOK1 (DOK1 Human)

Tags

mScarlet

Expression

Mammalian

Promoter

CMV

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pENTR2b-PTPN12(D199A,C231S)

Plasmid

#215516

Purpose

Gateway entry vector with a phosphatase-inactive PTP-PEST mutant (D199A,C231S)

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

PTPN12 (PTPN12 Human)

Use

Gateway entry vector

Mutation

D199A, C231S (Phosphatase inactive)

Promoter

none

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pINDUCER20-Src(E378G)

Plasmid

#215466

Purpose

Dox-inducible lentiviral transfer vector with Src constitutively-active mutant (E378G)

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

Src (SRC Chicken)

Use

Lentiviral

Mutation

E378G (constitutively-active)

Promoter

mCMV, Doxycycline-inducible

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pDEST-Dok1-V2

Plasmid

#215452

Purpose

Expression vector with Dok1 tagged with the BiFC V2 fragment

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

DOK1 (DOK1 Human)

Tags

Venus fragment 2 (V2)

Expression

Mammalian

Promoter

CMV

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pENTR2b-Dok1-Clover

Plasmid

#215455

Purpose

Gateway entry vector with Dok1 tagged with Clover

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

DOK1 (DOK1 Human)

Use

Gateway entry vector

Tags

Clover

Promoter

none

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pEF.DEST51-Dok1-Clover

Plasmid

#215456

Purpose

Expression vector with Dok1 tagged with Clover

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

DOK1 (DOK1 Human)

Tags

Clover

Expression

Mammalian

Promoter

EF1a

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pEF.DEST51-Dok1

Plasmid

#215458

Purpose

Expression vector with Dok1

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

DOK1 (DOK1 Human)

Expression

Mammalian

Promoter

EF1a

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
CAG-mRuby2-bGH

Plasmid

#235296

Purpose

ComMAND base gene regulating mRuby2, CAG-driven

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

CAG (synthetic cytomegalovirus early enhancer + c…

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a-TS.FF4x1-mRuby2(miR.FF4)-bGH

Plasmid

#235285

Purpose

ComMAND closed-loop circuit regulating mRuby2 with miR-FF4 design 2

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a-TS.FF4x1-mRuby2(miRE.FF4)-bGH

Plasmid

#235289

Purpose

ComMAND closed-loop circuit regulating mRuby2 with miRE-FF4 design 2

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge