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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 16,208 results for: grna

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Showing: 7521 - 7540 of 16208 results

  2. pBXMCS-2 Pconstitutive-sgRNA(Spa)_ctrA

    Plasmid
    #133342
    Purpose
    for constitutive expression of a single guide RNA from Streptococcus pasteurianus with a seed region that targets ctrA gene's promoter from Caulobacter crescentus
    Depositor
    Michael Laub
    Publication
    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA_ctrA
    Use
    CRISPR
    Promoter
    constitutive
    Available since
    Jan. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    133342-plasmid-map-sequence-id-269536

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  3. pBXMCS-2 Pconstitutive-sgRNA(Sth3)_blaA- Pconstitutive-sgRNA(Sth3)_cpaA

    Plasmid
    #133348
    Purpose
    expression of two sgRNA from Streptococcus thermophilus #3 each express from its own constitutive promoter; here first one targets blaA and second one targets cpaA (from Caulobacter crescentus)
    Depositor
    Michael Laub
    Publication
    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:
    Insert
    sgRNA_blaA and sgRNA_cpaA
    Use
    CRISPR
    Promoter
    constitutive
    Available since
    Jan. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    133348-plasmid-map-sequence-id-267409

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  4. pShHELIX_sgRNA_entry (CJT32)

    Plasmid
    #181782
    Purpose
    Expresses ShHELIX containing a nicking I-AniI fusion to ShTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Publication
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    nAniI-ShTnsB, ShTnsC, ShTniQ, ShCas12k
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    181782-plasmid-map-sequence-id-354808

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  6. SaCas9_sgRNA_expression_in_pBluescript

    Plasmid
    #122090
    Purpose
    U6 driven SaCas9 sgRNA expression vector for cloning own guides
    Depositor
    Scot Wolfe
    Publication
    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SaCas9 tracr (NEWENTRY S. pyogenes, Synthetic)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    April 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    122090-plasmid-map-sequence-id-242296

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  12. pLR05_pGuide_B2M_sgRNA1

    Plasmid
    #131090
    Purpose
    sgRNA for SpCas9 mediated B2M KO
    Depositor
    Xiaojun Lian
    Insert
    B2M KO sgRNA 1 (B2M Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Oct. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    131090-plasmid-map-sequence-id-256909

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  13. pLR06_pGuide_B2M_sgRNA2

    Plasmid
    #131091
    Purpose
    sgRNA for SpCas9 mediated B2M KO
    Depositor
    Xiaojun Lian
    Insert
    B2M KO sgRNA 2 (B2M Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Oct. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    131091-plasmid-map-sequence-id-256596

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  16. pX459_MCM2_sgRNA

    Plasmid
    #232730
    Purpose
    Generates MCM2 dTAG C ternimal knock-in cell lines
    Depositor
    Junjie Chen
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MCM2 (MCM2 Human)
    Expression
    Bacterial and Mammalian
    Mutation
    NO
    Available since
    March 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    232730-plasmid-map-sequence-id-465451

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  17. pX459_CDC45_sgRNA

    Plasmid
    #232732
    Purpose
    Generates CDC45 dTAG C ternimal knock-in cell lines
    Depositor
    Junjie Chen
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CDC45 (CDC45 Human)
    Expression
    Bacterial and Mammalian
    Mutation
    NO
    Available since
    March 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    232732-plasmid-map-sequence-id-465449

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Showing: 7521 - 7540 of 16208 results