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pMaCTag-28

Plasmid

#120007

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with 3xHA

Depositor

Michael Knop

Publication

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

3xHA

Use

Pcr template

Tags

3xHA

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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BCJJ180

Plasmid

#117516

Purpose

Cas9_4 Golden Gate Level 0

Depositor

Jonathan D Jones

Publication

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BsaI:AATG:Cas9_4:GCTT:BsaI

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Plant

Available since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pRL014

Plasmid

#250165

Purpose

DGRec 2-plasmid system: encodes bRT and avd.

Depositor

David Bikard

Publication

Rochette et al Nat Biotechnol. 2026 Apr 16. doi: 10.103

Insert

bRT (brt Bordetella phage BPP-1)

Expression

Bacterial

Available since

Aug. 11, 2026

Availability

Academic Institutions and Nonprofits only

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pRL038

Plasmid

#250169

Purpose

DGRec 2 plasmid-system: encodes bRT, avd, and a dgrRNA cloning site (containing ccdB toxin, to replace with TR). Allows dual targeting in combination with pRL021.

Depositor

David Bikard

Publication

Rochette et al Nat Biotechnol. 2026 Apr 16. doi: 10.103

Insert

bRT (brt Bordetella phage BPP-1)

Expression

Bacterial

Available since

Aug. 11, 2026

Availability

Academic Institutions and Nonprofits only

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pBAD-luxOFP

Plasmid

#229945

Purpose

Bacterial expression of a de novo designed, FRET-based neoLux1.2-CyOFP1 fusion bioluminescent probe (max. Em=588)

Depositor

Andy Yeh

Publication

Chen et al Chem. 2025 Mar 13;11(3):102346. doi: 10.

Insert

luxOFP

Tags

His-tag

Expression

Bacterial

Promoter

araBAD

Available since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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Ac5-STABLE2-blast

Plasmid

#208034

Purpose

Enables expression of 2 ORFs (with or without tags) and selection with blasticidin in insect cells. Includes Flag-mCherry-T2A-GFP-T2A-blast with Actin5C promoter.

Depositor

Rosa Barrio, James Sutherland

Publication

Gonzalez et al Sci Rep. 2011;1:75. doi: 10.1038/srep000

Type

Empty backbone

Expression

Insect

Available since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pHygO2r

Plasmid

#200079

Purpose

hygR selection unmarked rapid RMCE integration vector

Depositor

Michael Nonet

Publication

Nonet et al Genetics. 2023 Apr 20:iyad072. doi: 10.1

Type

Empty backbone

Use

Recombination mediated cassette exchange

Expression

Worm

Available since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pHygG2r

Plasmid

#200069

Purpose

hygR selection nls-mNG marked rapid RMCE integration vector

Depositor

Michael Nonet

Publication

Nonet et al Genetics. 2023 Apr 20:iyad072. doi: 10.1

Type

Empty backbone

Use

Recombination mediated cassette exchange

Expression

Worm

Available since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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ptdTomato.LIC.HXGPRT

Plasmid

#83365

Purpose

Plasmid contains LIC cassette upstream of an in-frame copy of tdTomato and HXGPRT selectable marker cassette for endogenous tagging on the gene locus

Depositor

Vernon Carruthers

Publication

Huynh et al Eukaryot Cell. 2009 Apr;8(4):530-9. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

tdTomato

Use

Toxoplasma gondii

Tags

tdTomato

Available since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pISH-Dlx5

Plasmid

#74344

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Dlx5. Not for gene expression.

Depositor

Peter Mombaerts

Publication

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

distal-less homeobox 5 (Dlx5 Mouse)

Available since

May 9, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pKSB-618 U6-sgRNA2/3 GGAA AACA

Plasmid

#173674

Purpose

To clone and express a gRNA in Anopheles

Depositor

Eric Marois

Type

Empty backbone

Use

CRISPR

Expression

Insect

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pAC-U63-QtgRNA2.1-8R

Plasmid

#170514

Purpose

gRNA cloning vector containing a Gal80 Co-CRISPR reporter for tissue-specific CRISPR in somatic tissues.

Depositor

Chun Han

Publication

Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118

Type

Empty backbone

Use

CRISPR

Expression

Insect

Promoter

U6:3

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET21-tVYN-Nt∆

Plasmid

#154431

Purpose

Plasmid used to express the tVYN-Nt∆ luminescent biosensor for use in live cell cyclic di-GMP detection

Depositor

Ming Hammond

Publication

Dippel et al ACS Chem Biol. 2020 Mar 25. doi: 10.1021

Insert

VenusΔC12-NtYcgRΔ-NLucΔ4

Expression

Bacterial

Promoter

T7

Available since

Aug. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pSETT4gap

Plasmid

#153413

Purpose

φC31-integrated plasmid including BsaI-recognition sites and T4-terminators surrounding the expression cassette

Depositor

Joern Kalinowski

Publication

Schaffert et al Microbiol Resour Announc. 2020 Sep 24;9(

Type

Empty backbone

Expression

Bacterial

Promoter

gapDH promoter from Eggerthella lenta

Available since

July 13, 2020

Availability

Academic Institutions and Nonprofits only

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P195

Plasmid

#139716

Purpose

Module plasmid, individual component of Fungal Bioluminescent Pathway, Npga

Depositor

Daniel Voytas

Publication

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

pMOD_A''_p35S:AsNpga:tAtug7

Use

Luciferase

Expression

Plant

Promoter

35S

Available since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pORFE0005

Plasmid

#112073

Purpose

AtDeadCAS9 D10A H840A without stop codon module CDS1ns

Depositor

Christian Chevalier

Insert

AtDeadCAS9 D10A H840A without stop codon

Use

CRISPR

Expression

Plant

Mutation

Plant codon optimized dCas9 D10A S. pyogenes

Available since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pNR160

Plasmid

#79542

Purpose

Output plasmid: contains 5-state register made up of TP901 and BxbI recognition sites.

Depositor

Timothy Lu

Publication

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Type

Empty backbone

Expression

Bacterial

Available since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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pISH-Dlx6

Plasmid

#74345

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Dlx6. Not for gene expression.

Depositor

Peter Mombaerts

Publication

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

distal-less homeobox 6 (Dlx6 Mouse)

Available since

May 9, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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MSGV Rh Acceptor

Plasmid

#64271

Purpose

Retroviral golden gate cloning vector for expression of functional Rhesus Macaque T-Cell Receptor genes

Depositor

David Ott

Publication

Coren et al Biotechniques. 2015 Mar 1;58(3):135-9. d

Type

Empty backbone

Use

Retroviral

Available since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCoofy36

Plasmid

#43994

Depositor

Sabine Suppmann

Publication

Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

Type

Empty backbone

Tags

His10 and S-OneStrep

Expression

Bacterial

Promoter

T7

Available since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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