We narrowed to 37,317 results for: kan

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AF1521-c003

Plasmid

#173111

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Publication

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

AF_1521 (AF_RS07675 Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126))

Tags

Nanoluc

Expression

Bacterial

Mutation

KH-M1-L192

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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L1_lacZgRNA-Ck2

Plasmid

#136137

Purpose

L1 in position 2, to clone gRNA target using BbsI, lacZ blue-white screening

Depositor

Jim Haseloff

Publication

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-MpU6:lacZgRNA

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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mILK-EFGP

Plasmid

#176896

Purpose

mouse ILK cloned into pEGFP-N1

Depositor

Sara Wickström

Publication

Martin et al Proc Natl Acad Sci U S A. 2022 Mar 15;11

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Integrin-linked kinase (Ilk Mouse)

Tags

EGFP

Expression

Mammalian

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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PtxS1-c003

Plasmid

#173076

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Publication

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

PtxS1

Expression

Bacterial

Mutation

D35-I221

Available since

Nov. 15, 2021

Availability

Academic Institutions and Nonprofits only

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3xmNeonGreen-3xrGBD

Plasmid

#176101

Purpose

Visualization of active Rho

Depositor

Dorus Gadella

Publication

Mahlandt et al J Cell Sci. 2021 Aug 6. pii: 271216. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

rhotekin (Rtkn Mouse)

Expression

Mammalian

Promoter

CMVdel

Available since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pN1-QUE37C

Plasmid

#129318

Purpose

ATP measurement

Depositor

Yasushi Okada

Publication

Yaginuma et al bioRxiv 2021.10.08.463131

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

QUEEN-37C

Expression

Mammalian

Available since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET41sIII_GST-ALC1

Plasmid

#173932

Purpose

Bacterial expression of ALC1

Depositor

Joan Conaway

Publication

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

ALC1 (CHD1L Human)

Tags

GST

Expression

Bacterial

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pME-PLIN2-tdTOMATO

Plasmid

#172533

Purpose

PLIN2-tdTOMATO fusion (zebrafish lipid droplet reporter) in middle entry vector for Gateway cloning

Depositor

Richard White

Publication

Lumaquin et al Elife. 2021 Jun 11;10. pii: 64744. doi:

Insert

plin2-tdtomato

Mutation

S80R, A81S, E144D, I148F, and R415H mutations in …

Available since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221-SLCO1B1_STOP

Plasmid

#161354

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Publication

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLCO1B1 (SLCO1B1 Human)

Expression

Mammalian

Available since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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p3E-TurboID

Plasmid

#166570

Purpose

Multisite Gateway 3' entry clone for fusions onto the N-terminus of TurboID. Parton lab clone JSS

Depositor

Rob Parton

Publication

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

TurboID

Use

Gateway Cloning

Available since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-eDHFR-MCS

Plasmid

#172108

Purpose

Mammalian expression of a protein of interest fused to the C-terminus of EGFP-eDHFR

Depositor

Shinya Tsukiji

Publication

Ishida et al J Am Chem Soc. 2013 Aug 28;135(34):12684

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

EGFP-eDHFR-20aa (folA E. coli)

Expression

Mammalian

Promoter

CMV

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pSF279

Plasmid

#167674

Purpose

Intermediate vector for five gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

Depositor

Niko Geldner

Publication

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

attL1-ccdB-attL2

Use

CRISPR

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pK27Sumo_His-SUMO-nsp8 (SARS-CoV-2)

Plasmid

#169187

Purpose

To express His-SUMO-nsp8 (SARS-CoV-2) in E. coli

Depositor

John Diffley

Publication

Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

Insert

14His-SUMO-nsp8 (ORF1ab Synthetic, SARS-CoV-2)

Tags

14His-SUMO (Ulp1-cleavable)

Expression

Bacterial

Mutation

Codon optimised for E. coli

Promoter

T5

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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LiPOP1

Plasmid

#168264

Purpose

A single-component optogenetic tool for photoswitchable necroptosis

Depositor

Yubin Zhou

Publication

He et al Adv Sci (Weinh). 2021 May 6;8(13):210042

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MLKL(1-125) (MLKL Human)

Tags

mCherry, CRY2-PHR

Expression

Mammalian

Mutation

H15A, K16A, R17A

Promoter

CMV

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJCX4

Plasmid

#170756

Purpose

Express in mammalian cells a target protein C-terminally fused to the Mxe GryA intein - chitin binding domain (CBD) tag.

Depositor

Roberto Dominguez

Publication

Carman et al J Biol Chem. 2021 Oct;297(4):101154. doi

Type

Empty backbone

Tags

Mxe GyrA intein - chitin binding domain (CBD)

Expression

Mammalian

Promoter

CMV

Available since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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EPS15-GFP-His

Plasmid

#170860

Purpose

Mammalian expression of EPS15-GFP-His.

Depositor

Justin Taraska

Publication

Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Epidermal growth factor receptor pathway substrate 15 (EPS15) (EPS15 Human)

Tags

(His)6 and EGFP

Expression

Mammalian

Promoter

CMV

Available since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pSEVA221AID-T7RNAP

Plasmid

#167977

Purpose

Expresses fusion protein AID-T7RNAP in E. coli

Depositor

Luis Ángel Fernández

Publication

Alvarez et al Nat Commun. 2020 Dec 22;11(1):6436. doi:

Insert

AID-T7RNAP

Tags

T7 tag and T7RNAP

Expression

Bacterial

Promoter

TetR-Ptet

Available since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221-GATA4

Plasmid

#101417

Purpose

Donor Vector containing GATA4 transcription factor, part of the Human TFome Collection

Depositor

George Church

Publication

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

GATA4 (GATA4 Human)

Use

Gateway Cloning

Mutation

Last nucleotide of stop codon removed to allow fo…

Available since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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10xHis-TEV-ATG5_ATG7_ATG10_ATG12_ATG16L1-TEV-StrepII (Human autophagy E3-like enzyme)

Plasmid

#169076

Purpose

Insect codon optimized ATG genes (excluded tags):10xHis-TEV-ATG5, ATG7, ATG10, ATG12 and ATG16L1-TEV-StrepII

Depositor

Sascha Martens

Publication

Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Inserts

ATG5 (ATG5 Human)
ATG7 (ATG7 Human)
ATG10 (ATG10 Human)
ATG12 (ATG12 Human)
ATG16 (ATG16L1 Human)

Tags

GGlinker, TEV cleavage site, StrepII and HIS10 Ta…

Expression

Bacterial and Insect

Promoter

Polyhedrin promoter

Available since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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2xmyc-JIP4

Plasmid

#164633

Purpose

Expresses JIP4 in mammalian cells with a 2xmyc tag

Depositor

Mark Cookson

Publication

Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

Insert

JIP4 (SPAG9 Human)

Tags

2xmyc

Expression

Mammalian

Promoter

CMV

Available since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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