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Showing: 1111 - 1140 of 5841 results

  3. pPBbsr2-Necl-5-ScNeo

    Plasmid
    #170283
    Purpose
    To monitor the status of Necl-5, the plasmid encodes a recombinant Necl-5 fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein
    Depositor
    Michiyuki Matsuda
    Article
    Ichise et al Elife. 2022 Feb 3;11. pii: 76269. doi: 1
    Insert
    a recombinant mouse Necl-5 fused to the mScarlet and to the mNeonGreen fluorescent protein (Pvr Mouse, Synthetic)
    Use
    Tags
    mScarlet and mNeonGreen
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    170283-plasmid-map-sequence-id-334387

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  4. C99m

    Plasmid
    #121003
    Purpose
    MoClo golden gate assembly CD part for codon-optimized mScarlet-I (fast-folding mScarlet variant, codon-optimized for E. coli; see https://www.fpbase.org/protein/mscarlet-i/).
    Depositor
    Richard Murray
    Insert
    mScarlet-I codon optimized
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121003-plasmid-map-sequence-id-236141

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  5. rk16s

    Plasmid
    #117436
    Purpose
    Expression of ARL4
    Depositor
    Richard Kahn
    Insert
    ARL4 (ARL4A Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    117436-plasmid-map-sequence-id-226665

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  6. TC9pAB

    Plasmid
    #71310
    Purpose
    used as a template for in vitro transcription of Cas9 mRNA after linearizing with BbsI
    Depositor
    Karl Degenhardt
    Insert
    Cas9
    Use
    In vitro transcription
    Tags
    NLS
    Expression
    Mutation
    Promoter
    T7
    Available since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    71310-plasmid-map-sequence-id-129410

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  7. C108m

    Plasmid
    #121010
    Purpose
    MoClo golden gate assembly CD part for mScarlet-I-pdt3 (fast-folding mScarlet variant, codon-optimized for E. col, with pdt3 degradation tag; see https://www.fpbase.org/protein/mscarlet-i/).
    Depositor
    Richard Murray
    Insert
    mScarlet-I codon optimized with pdt3 tag
    Use
    Synthetic Biology
    Tags
    pdt3 mfLon degradation tag
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121010-plasmid-map-sequence-id-236173

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  8. C103m

    Plasmid
    #121007
    Purpose
    MoClo golden gate assembly CD part for mScarlet-I-LAA (fast-folding mScarlet variant, codon-optimized for E. col, with LAA degradation tag; see https://www.fpbase.org/protein/mscarlet-i/).
    Depositor
    Richard Murray
    Insert
    mScarlet-I codon optimized with LAA tag
    Use
    Synthetic Biology
    Tags
    LAA degradation tag for ssrA
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121007-plasmid-map-sequence-id-236162

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  9. rk18s

    Plasmid
    #117438
    Purpose
    Expression of ARL11
    Depositor
    Richard Kahn
    Insert
    ARL11 (ARL11 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    C148R (see comments below)
    Promoter
    Available since
    Nov. 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    117438-plasmid-map-sequence-id-226383

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  10. TC9nickpAB

    Plasmid
    #71311
    Purpose
    used as a template for in vitro transcription of Cas9 nickase mRNA after linearizing with BbsI
    Depositor
    Karl Degenhardt
    Insert
    Cas9 nickase
    Use
    In vitro transcription
    Tags
    NLS
    Expression
    Mutation
    D10A nickase
    Promoter
    T7
    Available since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    71311-plasmid-map-sequence-id-131863

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  13. pKSII/INSeGFP

    Plasmid
    #53787
    Purpose
    1 kb zebrafish insulin promoter driving eGFP
    Depositor
    Larry Moss
    Article
    diIorio et al Dev Biol. 2002 Apr 1;244(1):75-84.
    Insert
    INS promoter/eGFP/pA
    Use
    Zebrafish
    Tags
    eGFP
    Expression
    Bacterial
    Mutation
    Promoter
    zebrafish insulin/ZIP
    Available since
    Jan. 7, 2015
    Availability
    Academic Institutions and Nonprofits only

  20. pC-GoldyTALEN

    Plasmid
    #38143
    Purpose
    destination vector for the Golden Gate TALEN kit, directs expression of TALENs from a truncated CAGs promoter
    Depositor
    Daniel Carlson
    Article
    Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 1.
    Insert
    GoldyTALEN
    Use
    Tags
    AcV5 and FokI homodimer
    Expression
    Mammalian
    Mutation
    Truncate at N and C terminous
    Promoter
    miniCaggs
    Available since
    July 30, 2012
    Availability
    Academic Institutions and Nonprofits only
    38143-plasmid-map-sequence-id-48254

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  27. pGD-γb

    Plasmid
    #196327
    Purpose
    35S promoter-driven expression of the barley stripe mosaic virus γb RNA silencing suppressor
    Depositor
    Zhenghe Li
    Article
    Ganesan et al J Virol. 2013 Oct;87(19):10598-611. doi:
    Insert
    barley stripe mosaic virus γb
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    duplicated cauliflower mosaic virus 35S promoter
    Available since
    May 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    196327-plasmid-map-sequence-id-390033

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  28. pCSIIpuro-Necl5-ScNeo

    Plasmid
    #209901
    Purpose
    To monitor the status of Necl-5, the plasmid encodes a recombinant Necl-5 fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein
    Depositor
    Michiyuki Matsuda
    Article
    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi
    Insert
    Necl5-ScNeo (Pvr Mouse)
    Use
    Lentiviral
    Tags
    mNeonGreen and mScarlet
    Expression
    Mutation
    Promoter
    Available since
    Nov. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    209901-plasmid-map-sequence-id-416655

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  29. pCSIIpuro-TGFa-ScNeo

    Plasmid
    #209895
    Purpose
    To monitor the status of TGFα, the plasmid encodes a recombinant TGFα fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein
    Depositor
    Michiyuki Matsuda
    Article
    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi
    Insert
    TGFa-ScNeo (TGFA Human)
    Use
    Lentiviral
    Tags
    GGGSGGGS linker, HA tag, mNeonGreen, and mScarlet…
    Expression
    Mutation
    Promoter
    Available since
    Nov. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    209895-plasmid-map-sequence-id-416585

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  30. pHiFi Cas9-2×sgRNA (empty, donor)

    Plasmid
    #162277
    Purpose
    All-in-one vector for CRISPR/Cas9-mediated homology-independent knock-in system. The plasmid contains BPNLS-HiFi Cas9-BPNLS and two sgRNA expression cassettes.
    Depositor
    Kazuhisa Nakayama
    Article
    Fujisawa et al Biol Open. 2021 Aug 27. pii: 271969. doi
    Insert
    HiFi Cas9
    Use
    CRISPR
    Tags
    BPNLS and FLAG tag
    Expression
    Mammalian
    Mutation
    SpCas9 (R691A)
    Promoter
    Available since
    Aug. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    162277-plasmid-map-sequence-id-319794

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Showing: 1111 - 1140 of 5841 results