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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 57,674 results for: Eng;

Showing: 1851 - 1900 of 57674 results

  14. pPPC010.AAV

    Plasmid
    #171175
    Purpose
    Expression of Sp.pCas9-dCas9, BBa_J23107-MCP-SoxS(R93A/S101A), and hAAVS1 scRNA on pBBR1-KmR plasmid
    Depositor
    Jesse Zalatan
    Article
    Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(
    Inserts
    hAAVS1 scRNA
    Sp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    SoxS has R93A and S101A mutations
    Promoter
    BBa_J23119 and Sp.pCas9, BBa_J23107
    Available Since
    Oct. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    171175-plasmid-map-sequence-id-344748

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  16. pUC57-4eCOL2A1

    Plasmid
    #97211
    Purpose
    For cloning of a COL2A1-based, chondrogenesis-responsive promoter, and subcloning this insert into new vectors
    Depositor
    Ryan Porter
    Article
    De La Vega et al Tissue Eng Part C Methods. 2019 Feb 6. d
    Insert
    4 repeats of COL2A1 intronic enhancer (+2126/+2174) upstream of core promoter (-164/+37) (COL2A1 Human)
    Use
    Vector is designed for cloning and generation of …
    Promoter
    COL2A1 regulatory elements
    Available Since
    Oct. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    97211-plasmid-map-sequence-id-181460

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  17. pAWPSG-Po-nCas9-BE

    Plasmid
    #195740
    Purpose
    Express APOVEC1-nCas9(D10A)-UGI using phenol inducible system in Methanotroph or E. coli
    Depositor
    Seung-Goo Lee
    Article
    Jeong et al Chem Eng J, 2023
    Insert
    Di-Methyl Phenol Regulatory protein / APOBEC1-nCas9(D10A)-UGI
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    Po (phoenol-inducible promoter)
    Available Since
    March 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    195740-plasmid-map-sequence-id-386428

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  19. pGF1-4eCOL2A1

    Plasmid
    #97210
    Purpose
    Lentiviral expression of copGFP-T2A-FLuc under control of a COL2A1-based, chondrogenesis-responsive promoter
    Depositor
    Ryan Porter
    Article
    De La Vega et al Tissue Eng Part C Methods. 2019 Feb 6. d
    Insert
    4 repeats of COL2A1 intronic enhancer (+2126/+2174) upstream of core promoter (-164/+37) (COL2A1 Human)
    Use
    Lentiviral and Luciferase
    Expression
    Mammalian
    Promoter
    COL2A1 regulatory elements
    Available Since
    Oct. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    97210-plasmid-map-sequence-id-182599

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  31. pAWPSG-Po-nCas9-BE- Ptac-sgRNA(mmoX_1)

    Plasmid
    #195741
    Purpose
    Base editing for making a pre-stop codon in mmoX using phenol inducible system
    Depositor
    Seung-Goo Lee
    Article
    Jeong et al Chem Eng J, 2023
    Insert
    Di-Methyl Phenol Regulatory protein / APOBEC1-nCas9(D10A)-UGI / Ptac-RiboJ-sgRNA(mmoX_1)
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    Po (phoenol-inducible promoter)
    Available Since
    March 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    195741-plasmid-map-sequence-id-386423

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  32. pAWPSG-Ptet-nCas9-BE

    Plasmid
    #195739
    Purpose
    Express APOVEC1-nCas9(D10A)-UGI using aTC inducible system in Methanotroph or E. coli
    Depositor
    Seung-Goo Lee
    Article
    Jeong et al Chem Eng J, 2023
    Insert
    Tet repressor protein / APOBEC1-nCas9(D10A)-UGI
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    Ptet(Tetracycline inducible protein)
    Available Since
    March 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    195739-plasmid-map-sequence-id-386424

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  34. Linked Free ClLIS1 (M)

    Plasmid
    #182487
    Purpose
    Yeast integrative plasmid for expressing fusion protein ERG20(F96W-N127W)-ClLIS1, with linker sequence AG4TGGA (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3)
    Depositor
    Claudia Vickers
    Article
    Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.
    Insert
    FPPS(M)-LS
    Use
    Synthetic Biology; Metabolic engineering
    Expression
    Yeast
    Promoter
    GAL10
    Available Since
    Nov. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    182487-plasmid-map-sequence-id-360431

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  36. p168-759

    Plasmid
    #62619
    Purpose
    ePathBrick pETM6 vector encoding CsF3H, AaDFR, DuLAR with Protein Scaffold GBD:SH3:PDZ (1:1:4)
    Depositor
    Mattheos Koffas
    Article
    Zhao et al Metab Eng. 2014 Dec 17;28C:43-53. doi: 1
    Inserts
    flavaone 3B-hydroxylase
    dihydroflavonol 4-reductase
    leucoanthocyanidin reductase
    Scaffold Protein encoding GBD:SH3:PDZ (1:1:4)
    Use
    Synthetic Biology
    Tags
    GBD, PDZ, and SH3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    July 9, 2015
    Availability
    Academic Institutions and Nonprofits only
    62619-plasmid-map-sequence-id-103510

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  41. Linked Free NES

    Plasmid
    #182485
    Purpose
    Yeast integrative plasmid for expressing fusion protein ERG20-AcNES1, with linker sequence AG4TGGA (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.
    Insert
    FPPS-NES
    Use
    Synthetic Biology; Metabolic engineering
    Expression
    Yeast
    Promoter
    GAL10
    Available Since
    Nov. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    182485-plasmid-map-sequence-id-360436

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  42. Linked Free PcPTS

    Plasmid
    #182486
    Purpose
    Yeast integrative plasmid for expressing fusion protein ERG20-PcPTS, with linker sequence AG4TGGA (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3)
    Depositor
    Claudia Vickers
    Article
    Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.
    Insert
    FPPS-PTS
    Use
    Synthetic Biology; Metabolic engineering
    Expression
    Yeast
    Promoter
    GAL10
    Available Since
    Nov. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    182486-plasmid-map-sequence-id-360437

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  43. Coexpressed Free GFP-NES

    Plasmid
    #182491
    Purpose
    Yeast integrative plasmid for expressing ERG20 (GAL10 promoter) and fusion protein GFP-AcNES1 (GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.
    Inserts
    FPPS
    GFP-NES
    Use
    Synthetic Biology; Metabolic engineering
    Expression
    Yeast
    Promoter
    GAL10 and GAL7
    Available Since
    Nov. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    182491-plasmid-map-sequence-id-360449

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Showing: 1851 - 1900 of 57674 results