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Showing: 1851 - 1900 of 28673 results

  16. dFP-mini

    Plasmid
    #120543
    Purpose
    Expresses dFP biliverdin-binding fluorescence-activating mini 10 KDa protein in bacteria.
    Depositor
    Brian Chow
    Article
    Sheehan et al Biochemistry. 2018 Nov 28. doi: 10.1021/
    Insert
    de novo fluorescence-activating protein mini (dFP-mini)
    Use
    Tags
    6xHis - TEV cleavage
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    120543-plasmid-map-sequence-id-234163

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  21. SHC003BSD

    Plasmid
    #133300
    Purpose
    The puromycin selection marker was replaced by blasticidin in SHC003. This construct could be used together with other plasmid contains puromycin selection marker.
    Depositor
    Jingshi Shen
    Article
    Wang et al J Biol Chem. 2019 Dec 27;294(52):19988-1
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    133300-plasmid-map-sequence-id-261777

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  22. pUBC_HA_2xPP7-VP64

    Plasmid
    #68418
    Purpose
    Transient expression of two tandem copies of the non-aggregating delta-FG mutant, fused to the VP64 transcription activator, in mammalian cells, under a UBC promoter
    Depositor
    John Rinn
    Article
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Insert
    PP7(deltaFG)
    Use
    Synthetic Biology
    Tags
    HA and VP64
    Expression
    Mammalian
    Mutation
    PP7: [delta]67_74 (non-aggregating)
    Promoter
    human UbC
    Available since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68418-plasmid-map-sequence-id-125550

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  33. pU6_(GLuc)_INT[Spinach2]

    Plasmid
    #68430
    Purpose
    Transient expression in mammalian cells of an "INT" construct_bearing the "Spinach2" aptamer, targeting the GLuc reporter. U6 promoter
    Depositor
    John Rinn
    Article
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Insert
    INT construct_bearing the "Spinach2" aptamer
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    human U6
    Available since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68430-plasmid-map-sequence-id-120208

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  39. LUC56 (Luc/UGA/SP)

    Plasmid
    #112147
    Purpose
    Mammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activity
    Depositor
    Paul Copeland
    Article
    Shetty et al J Biol Chem. 2014 Sep 5;289(36):25317-26
    Insert
    Luciferase coding region with Cysteine 258 mutated to selenocysteine (U) and wild-type SELENOP 3'UTR (Selenop Photinus pyralis, Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Cysteine 258mutated to selenocysteine (U)
    Promoter
    Available since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112147-plasmid-map-sequence-id-214894

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Showing: 1851 - 1900 of 28673 results