We narrowed to 21,330 results for: 034

1. HIsGB1-FusionL63A + CBP TAZ1(340-439)

   Plasmid

   #173764

   Purpose

   Bacterial coexpression of CBP TAZ1 with CITED2/Hif1a activation domain constructs

   Depositor

   Peter Wright

   Article

   Appling et al Structure. 2021 Sep 11. pii: S0969-2126(

   Inserts

   CITED2(216-246):Hif1a(796-826L822A) (HIF1A, CITED2 Human)
   CBP (340-439) (Crebbp Mouse)
   

   Use

   Tags

   His6GB1 and thrombin

   Expression

   Bacterial

   Mutation

   fusion of CITED2 and Hif1a

   Promoter

   T7

   Available Since

   Feb. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCRII HSPB7

   Plasmid

   #61034

   Purpose

   Probe for HSPB7 for in situ hybridization in zebrafish

   Depositor

   Lara Hutson

   Article

   Marvin et al Dev Dyn. 2008 Feb;237(2):454-63.

   Insert

   HSPB7 (hspb7 Zebrafish)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

3. TRUPATH Triple GaZ

   Plasmid

   #196053

   Purpose

   Encodes a G alpha subunit (GNAZ) with RLuc8, a G gamma subunit (GNG1) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

   Depositor

   Justin English

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Inserts

   GAlphaz-RLuc8 (GNAZ Human)
   GGamma1-GFP2 (GNGT1 Human)
   GBeta3 (GNB3 Human)
   

   Use

   Luciferase

   Tags

   GFP2 and GSAG linker

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available Since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. CLYBL-(Ef1a-SBP-LNGFR-T2A-mApple)-(CAG-rtTA)-(TRE-hNIL))

   Plasmid

   #105842

   Purpose

   Donor construct for introduction of hNIL factors and SBP-LNGFR for streptavidin bead enrichment

   Depositor

   Michael Ward

   Article

   Fernandopulle et al Curr Protoc Cell Biol. 2018 Jun;79(1):e5

   Inserts

   Neurogenin 2 (NEUROG2 Human)
   ISL LIM Homeobox 1 (ISL1 Human)
   LIM Homeobox 3 (LHX3 Human)
   Optimized mApple
   rtTA3G
   SBP Delta-LNGFR
   

   Use

   CRISPR and TALEN

   Tags

   Expression

   Mutation

   Promoter

   CAG, EF-1alpha, and TRE3G

   Available Since

   April 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. TRUPATH Triple Gagust

   Plasmid

   #196054

   Purpose

   Encodes a G alpha subunit (GNAT3) with RLuc8, a G gamma subunit (GNG1) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

   Depositor

   Justin English

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Inserts

   GAlphaGustducin-RLuc8 (GNAT3 Human)
   GGamma1-GFP2 (GNGT1 Human)
   GBeta3 (GNB3 Human)
   

   Use

   Luciferase

   Tags

   GFP2 and GSAG linker

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available Since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-EF1a-HIF2a dPA (P405A, P531A)

   Plasmid

   #232954

   Purpose

   Stably express a constitutively stable version of HIF2a (HIF2a dPA)

   Depositor

   William Kaelin

   Article

   Jiang et al Cell. 2025 Feb 25:S0092-8674(25)00156-4.

   Insert

   HIF2a (EPAS1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   P405A, P531A

   Promoter

   EF1a

   Available Since

   March 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIRES-mCerulean-GAI1-92-KRas/N12-KRas/13C(Q61L)-GID1-mVenus-CAAX

   Plasmid

   #232262

   Purpose

   Express split-KRas fragments that are fused with CIDs and FPs

   Depositor

   Cliff Stains

   Article

   He et al ACS Chem Biol. 2025 Aug 26. doi: 10.1021

   Inserts

   mCerulean-GAI1-92-KRas/N12 (KRAS Human)
   KRas/13C(Q61L)-GID1-mVenus-CAAX (KRAS Human)
   

   Use

   Tags

   mCerulean and mVenus

   Expression

   Mammalian

   Mutation

   KRas-Q61L

   Promoter

   CMV

   Available Since

   Sept. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pIRES-mCerulean-GAI1-92-KRas/N12-KRas/13C(S17N)-GID1-mVenus-CAAX

   Plasmid

   #232263

   Purpose

   Express split-KRas fragments that are fused with CIDs and FPs

   Depositor

   Cliff Stains

   Article

   He et al ACS Chem Biol. 2025 Aug 26. doi: 10.1021

   Inserts

   mCerulean-GAI1-92-KRas/N12 (KRAS Human)
   KRas/13C(S17N)-GID1-mVenus-CAAX (KRAS Human)
   

   Use

   Tags

   mCerulean and mVenus

   Expression

   Mammalian

   Mutation

   KRas-S17N

   Promoter

   CMV

   Available Since

   Sept. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBIG1a-PAF1CdeltaWDR61

   Plasmid

   #231745

   Purpose

   Protein expression in insect cells

   Depositor

   Jesper Svejstrup

   Article

   Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

   Inserts

   CTR9 (CTR9 Human)
   CDC73 (CDC73 Human)
   PAF1 (PAF1 Human)
   LEO1 (LEO1 Human)
   

   Use

   Tags

   3C-Strep(II) and Flag-3C

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   May 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. KHBD00208

    Plasmid

    #34359

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG10348 (CG10348 Fly)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pUC18_BCR_GUSB_ABL1_e14a3

    Plasmid

    #190885

    Purpose

    molecular monitoring of atypical BCR::ABL1 fusion transcripts

    Depositor

    Nicholas Cross

    Article

    Schafer et al J Cancer Res Clin Oncol. 2021 Oct;147(10

    Inserts

    e14a3 BCR::ABL1 fusion transcript
    ABL1 cDNA (ABL1 Human)
    GUSB cDNA (GUSB Human)
    BCR (BCR Human)
    

    Use

    Cloned cdna transcripts for use in rt-qpcr bcr::a…

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. FoxO6deltaCt

    Plasmid

    #124486

    Purpose

    Expresses dominant negative form of mouse FoxO6 with deleted C-term transactivation domain. T26A/S184A mutations increase nuclear localization of FoxO6.

    Depositor

    Anne Brunet

    Article

    Salih et al Genes Dev. 2012 Dec 15;26(24):2780-801.

    Insert

    FoxO6 (Foxo1, Foxo4, Foxo3, Foxo6 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    T26A, S184A, transactivation domain deleted from …

    Promoter

    Available Since

    June 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEMS1161

    Plasmid

    #29076

    Purpose

    Expression of the eGFP reporter gene was not detected in the brain and eye.

    Depositor

    Elizabeth Simpson

    Article

    Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

    Insert

    Ple93 (GPR88 Human)
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    EGFP-NLS

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEMS1163

    Plasmid

    #29078

    Purpose

    Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested

    Depositor

    Elizabeth Simpson

    Article

    Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

    Insert

    Ple95 (GPR88 Human)
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    EGFP-NLS

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEMS1160

    Plasmid

    #29075

    Purpose

    Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested

    Depositor

    Elizabeth Simpson

    Article

    Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

    Insert

    Ple92 (GPR88 Human)
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    EGFP-NLS

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 3, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. FSW_jGCaMP8f

    Plasmid

    #197034

    Purpose

    Express GCaMP8f from hSyn1 promoter

    Depositor

    Ronald Hart

    Insert

    GCaMP8f
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTpPBR11_accNAT

    Plasmid

    #154034

    Purpose

    Diatom episome backbone for T. pseudonana or C. crytpica

    Depositor

    Raffaela Abbriano

    Type

    Empty backbone

    Use

    Diatom cloning

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-HA

    Plasmid

    #128034

    Purpose

    Mammalian expression vector with N terminal HA tag

    Depositor

    Oskar Laur

    Type

    Empty backbone

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEP4 E02S CK2M EN2L

    Plasmid

    #20924

    Purpose

    used in the derivation of human iPS cells using non-integrating episomal vectors; expresses Oct4 and Sox2; Klf4 and Myc; Nanog and Lin28

    Depositor

    James Thomson

    Article

    Yu et al Science. 2009 Mar 26.

    Insert

    Oct4 and Sox2; Nanog and Lin28; Klf4 and Myc (MYC, SOX2, KLF4, NANOG, POU5F1, LIN28A Human)
    

    Use

    Episomal/ebna

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 20, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMaster1

    Plasmid

    #58525

    Purpose

    Reprogram somatic cells to iPS cells

    Depositor

    Mario Capecchi

    Article

    Wu et al Proc Natl Acad Sci U S A. 2014 Jul 22;11

    Inserts

    neo
    tk
    oct4 (POU5F1 Human)
    Klf4 (KLF4 Human)
    Sox2 (SOX2 )
    myc (MYC Human)
    EBNA-1
    Amp
    Lin28 (LIN28A Human)
    Nanog (NANOG Human)
    IRES
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    CAG and EF1a

    Available Since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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