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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 741 - 760 of 22720 results

  1. pPNT6-eGFP

    Plasmid
    #71303
    Purpose
    This vector can be used for homologous recombination and allows to obtain recombinant neomycin resistant fluorescent cells that can loose fluorescence after Cre expression
    Depositor
    Marco Musso, Gian Luca Forni
    Type
    Empty backbone
    Use
    Cre/Lox
    Expression
    Mammalian
    Available Since
    March 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    71303-plasmid-map-sequence-id-130367

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  8. pDH6

    Plasmid
    #83777
    Purpose
    Useful for tagging yeast proteins with C-terminal YFP tag; G418 selection
    Depositor
    Eric Muller
    Article
    Hailey et al Methods Enzymol. 2002;351:34-49.
    Insert
    YFP
    Tags
    YFP
    Expression
    Yeast
    Mutation
    S65G, V68L, Q69K, S72A, T203Y mutations added.
    Available Since
    Sept. 30, 2016
    Availability
    Academic Institutions and Nonprofits only
    83777-plasmid-map-sequence-id-160009

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  13. pfMDH_H303A

    Plasmid
    #67730
    Purpose
    Expresses H303A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli
    Depositor
    Justin Siegel
    Article
    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:
    Insert
    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    Tags
    6x-His tag
    Expression
    Bacterial
    Mutation
    Changed histidine 303 to alanine
    Promoter
    T7
    Available Since
    Sept. 11, 2015
    Availability
    Academic Institutions and Nonprofits only

  14. pfMDH_D230A

    Plasmid
    #67725
    Purpose
    Expresses D230A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli
    Depositor
    Justin Siegel
    Article
    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:
    Insert
    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    Tags
    6x-His tag
    Expression
    Bacterial
    Mutation
    Changed aspartate 230 to alanine
    Promoter
    T7
    Available Since
    Sept. 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  15. pfMDH_N191A

    Plasmid
    #67724
    Purpose
    Expresses N191A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli
    Depositor
    Justin Siegel
    Article
    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:
    Insert
    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    Tags
    6x-His tag
    Expression
    Bacterial
    Mutation
    Changed asparagine 191 to alanine
    Promoter
    T7
    Available Since
    Sept. 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  16. pfMDH_K295A

    Plasmid
    #67726
    Purpose
    Expresses K295A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli
    Depositor
    Justin Siegel
    Article
    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:
    Insert
    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    Tags
    6x-His tag
    Expression
    Bacterial
    Mutation
    Changed lysine 295 to alanine
    Promoter
    T7
    Available Since
    Sept. 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  18. pDH5

    Plasmid
    #83775
    Purpose
    Useful for tagging yeast proteins with C-terminal YFP tag; his selection
    Depositor
    Eric Muller
    Article
    Hailey et al Methods Enzymol. 2002;351:34-49.
    Insert
    YFP
    Tags
    YFP
    Expression
    Yeast
    Mutation
    S65G, V68L, Q69K, S72A, T203Y. Also includes Q80R…
    Available Since
    Oct. 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    83775-plasmid-map-sequence-id-159997

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Showing: 741 - 760 of 22720 results