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pNIC28-Bsa4

Plasmid

#26103

Purpose

SGC Empty backbone for bacterial expression

Depositor

Opher Gileadi

Publication

Savitsky et al J Struct Biol. 2010 Oct;172(1):3-13. doi

Type

Empty backbone

Tags

6xHis and TEV cleavage site

Expression

Bacterial

Promoter

T7-lacO (lactose/IPTG inducible)

Available since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

42 Articles

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pUCmini-iCAP-PHP.S

Plasmid

#103006

Purpose

non-standard AAV2 rep-AAV-PHP.S cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

Depositor

Viviana Gradinaru

Publication

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

Synthetic construct isolate AAV-PHP.S VP1 gene

Use

AAV

Expression

Mammalian

Promoter

p41

Available since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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eGFP STAT1 S727A

Plasmid

#12304

Depositor

Alan Perantoni

Publication

Timofeeva et al Oncogene. 2006 Dec 7;25(58):7555-64. doi

Insert

STAT1 S727A (STAT1 Human)

Tags

eGFP

Expression

Mammalian

Mutation

S727A

Available since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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eGFP STAT1 Y701F

Plasmid

#12302

Depositor

Alan Perantoni

Publication

Timofeeva et al Oncogene. 2006 Dec 7;25(58):7555-64. doi

Insert

STAT1 Y701F (STAT1 Human)

Tags

eGFP

Expression

Mammalian

Mutation

Y701F

Available since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
pMJ825

Plasmid

#39315

Depositor

Jennifer Doudna

Publication

Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

Insert

Cas9

Use

CRISPR

Tags

His6 and MBP

Expression

Bacterial

Mutation

Aspartate 10 to Alanine (D10A)

Promoter

T7

Available since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pAAV-hSynapsin-Flpo

Plasmid

#60663

Purpose

Expresses pAAV-hSynapsin-Flpo

Depositor

Massimo Scanziani

Publication

Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

Insert

Flpo

Use

AAV

Promoter

hSynapsin1

Available since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pIRES2-EGFP-p53 WT

Plasmid

#49242

Purpose

expresses human wt p53 and EGFP in mammalian cells

Depositor

Dylan Taatjes

Publication

Lin et al Aging Cell. 2013 Oct;12(5):863-72. doi:

Insert

p53 (TP53 Human)

Tags

IRES-EGFP

Expression

Mammalian

Promoter

CMV

Available since

Dec. 27, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pCA24N-p50-ligase

Plasmid

#87742

Purpose

IPTG-inducible expression of p50-ligase fusion for protein purification

Depositor

Wayne Patrick

Publication

Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

Insert

T4 DNA ligase (30 )

Tags

6xHis and human p50/NF-kB (amino acids 40-366)

Expression

Bacterial

Promoter

T5-lac

Available since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-Ptet-RFP-shR-rtTA

Plasmid

#35625

Purpose

Tet-inducible AAV shRNA vector to test efficacy of shRNAs. Used in combination with pGFPns-reporter (Addgene plasmid #35626).

Depositor

Howard Gu

Publication

Naughton et al Anal Biochem. 2011 Oct 1;417(1):162-4. E

Type

Empty backbone

Use

AAV and RNAi; Tet inducible

Expression

Mammalian

Promoter

Ptet

Available since

April 19, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCA24N-ligase

Plasmid

#87741

Purpose

IPTG-inducible expression of T4 DNA ligase for protein purification

Depositor

Wayne Patrick

Publication

Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

Insert

T4 DNA ligase (30 )

Tags

6xHis

Expression

Bacterial

Promoter

T5-lac

Available since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-jGCaMP7s

Plasmid

#104463

Purpose

Mammalian expression of ultrasensitive protein calcium sensor

Depositor

Douglas Kim, GENIE Project

Publication

Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

Insert

jGCaMP7s

Tags

T7 epitope, Xpress tag, 6xHis

Expression

Mammalian

Promoter

CMV

Available since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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eGFP STAT1 S727A

Plasmid

#12304

Depositor

Alan Perantoni

Publication

Timofeeva et al Oncogene. 2006 Dec 7;25(58):7555-64. doi

Insert

STAT1 S727A (STAT1 Human)

Tags

eGFP

Expression

Mammalian

Mutation

S727A

Available since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pAAV-hSynapsin-Flpo

Plasmid

#60663

Purpose

Expresses pAAV-hSynapsin-Flpo

Depositor

Massimo Scanziani

Publication

Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

Insert

Flpo

Use

AAV

Promoter

hSynapsin1

Available since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pAAV-syn-dLight1.3b

Plasmid

#135762

Purpose

For expression of the genetically encoded dopamine sensor dLight1.3b

Depositor

Lin Tian

Publication

Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

Has service

AAV9

Insert

dLight1.3b

Use

AAV

Tags

Flag tag

Expression

Mammalian

Promoter

Synapsin

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pMJ825

Plasmid

#39315

Depositor

Jennifer Doudna

Publication

Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

Insert

Cas9

Use

CRISPR

Tags

His6 and MBP

Expression

Bacterial

Mutation

Aspartate 10 to Alanine (D10A)

Promoter

T7

Available since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pAAV-EF1a-Con/Fon-GCaMP6M (AAV8)

Viral prep

#137119-AAV8

Purpose

Ready-to-use AAV8 particles produced from pAAV-EF1a-Con/Fon-GCaMP6M (#137119). In addition to the viral particles, you will also receive purified pAAV-EF1a-Con/Fon-GCaMP6M plasmid DNA. EF1a-driven, Cre and Flp-dependent expression of GCaMP6M. These AAV preparations are suitable purity for injection into animals.

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Publication

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Promoter

EF1a

Available since

Sept. 24, 2020

Availability

Academic Institutions and Nonprofits only
pLV-EF1a-IRES-Puro

Plasmid

#85132

Purpose

lentiviral backbone

Depositor

Tobias Meyer

Publication

Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

Has service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Promoter

EF1a

Available since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

94 Articles

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pUCmini-iCAP-PHP.S

Plasmid

#103006

Purpose

non-standard AAV2 rep-AAV-PHP.S cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

Depositor

Viviana Gradinaru

Publication

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

Synthetic construct isolate AAV-PHP.S VP1 gene

Use

AAV

Expression

Mammalian

Promoter

p41

Available since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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pEGFP-p150Glued

Plasmid

#36154

Depositor

David Stephens

Publication

Watson et al J Cell Sci. 2006 Jul 1;119(Pt 13):2758-6

Insert

p150Glued (DCTN1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

see documentation

Available since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pCA24N-ligase

Plasmid

#87741

Purpose

IPTG-inducible expression of T4 DNA ligase for protein purification

Depositor

Wayne Patrick

Publication

Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

Insert

T4 DNA ligase (30 )

Tags

6xHis

Expression

Bacterial

Promoter

T5-lac

Available since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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