We narrowed to 1,120 results for: rbis

1. pCDNA3-Flag-SPRTN-wt

   Plasmid

   #110214

   Purpose

   Expression in mammalian cells of full-length wild-type SPRTN protein with a Flag-tag at N-terminus.

   Depositor

   Kristijan Ramadan, Ivana Marinovic-Terzic

   Article

   Lessel et al Nat Genet. 2014 Nov;46(11):1239-44. doi:

   Insert

   SPRTN (SPRTN Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   P296L (see depositor comments below)

   Promoter

   Available since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGFP-N1-HSD17B13-WT-FLAGC

   Plasmid

   #184501

   Purpose

   Expresses human HSD17B13 with a C-terminal FLAG-GFP tag. Confers G418 resistance.

   Depositor

   Sébastien Britton

   Article

   Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

   Insert

   17-beta-hydroxysteroid dehydrogenase 13 (HSD17B13 Human)
   

   Use

   Tags

   FLAGC-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFP-N1-HSD17B11-WT-FLAGC

   Plasmid

   #161903

   Purpose

   Expresses human HSD17B11 with a C-terminal FLAG-GFP tag. Confers G418 resistance.

   Depositor

   Sébastien Britton

   Article

   Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

   Insert

   hydroxysteroid 17-beta dehydrogenase 11 (HSD17B11 Human)
   

   Use

   Tags

   FLAG-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. AICSDP-45: TTN-mEGFP

   Plasmid

   #114412

   Purpose

   Homology arms and linker-mEGFP sequence for C-terminus tagging of human TTN, via Cas9-excisable CAGGS-mCherry selection cassette

   Depositor

   Allen Institute for Cell Science

   Article

   Roberts et al Stem Cell Reports. 2019 May 14;12(5):114

   Insert

   TTN Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (TTN Human)
   

   Use

   CRISPR; Donor template

   Tags

   linker-mEGFP

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Promoter

   Available since

   Sept. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBRPyCAG-Mettl3-dsRed-IRES-puro

   Plasmid

   #61516

   Purpose

   Constitutive expression of murine wild-type Mettl3

   Depositor

   Jacob Hanna

   Article

   Geula et al Science. 2015 Feb 27;347(6225):1002-6. d

   Insert

   Mettl3 (Mettl3 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAGG

   Available since

   Jan. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGL3-human OCT4 DE-SV40-Luc

   Plasmid

   #52414

   Purpose

   Luciferase reporter plasmid for DE OCT4 enhancer activity

   Depositor

   Jacob Hanna

   Article

   Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

   Insert

   Distal element of human OCT4 promoter (POU5F1 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. PBRPyCAG-LoxP-ERAS-STOPcassette-Loxp-DsRed-IRES-PURO

   Plasmid

   #52419

   Purpose

   Constitutive mammalian expression of ERAS. Upon CRE mediated deletion of ERAS insert, dsRED expression markers is truned on due to concomittant removal of stop cassette.

   Depositor

   Jacob Hanna

   Article

   Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

   Insert

   ERAS (ERAS Human)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   CAGGS

   Available since

   Sept. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP-C1-FLAG-BRAT1

   Plasmid

   #161920

   Purpose

   Expresses human BRAT1 with a N-terminal GFP-FLAG tag. Confers G418 resistance.

   Depositor

   Sébastien Britton

   Article

   Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

   Insert

   BRCA1-associated ATM activator 1 (BRAT1 Human)
   

   Use

   Tags

   GFP-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-N1-HPGD-FLAGC

   Plasmid

   #161915

   Purpose

   Expresses human HPGD with a C-terminal FLAG-GFP tag. Confers G418 resistance.

   Depositor

   Sébastien Britton

   Article

   Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

   Insert

   HPGD (HPGD Human)
   

   Use

   Tags

   FLAG-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNTK Gatad2a flox

    Plasmid

    #110812

    Purpose

    Donor targeting vector for generating mouse Gatad2a conditional knockout allele

    Depositor

    Jacob Hanna

    Article

    Mor et al Cell Stem Cell. 2018 Sep 6;23(3):412-425

    Insert

    mGataD2a (Gatad2a Mouse)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pENTR1A_mmFbxw7(res) (closed)

    Plasmid

    #160105

    Purpose

    Expresses murine Fbxw7 alpha with a T199 silent mutation in mammalian cells. The T199 silent mutation confers resistance to the 5'-TGAACATGGTACAAGGCCAG-3' sgRNA

    Depositor

    Rizwan Haq

    Article

    Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

    Insert

    Fbxw7alpha (Fbxw7 Mouse)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pB-CuR-hSOD1

    Plasmid

    #232477

    Purpose

    The inducible PiggyBac Cumate Switch vector (PBQM812A-1 System Biosciences) expressing human SOD1gene including flag -tag (DYKDDDDK) on the 3' end

    Depositor

    Lukas Trantirek

    Article

    Rynes et al Commun Biol. 2025 Feb 7;8(1):194. doi: 1

    Insert

    Homo sapiens superoxide dismutase 1 (SOD1 Human)
    

    Use

    Transposon system

    Tags

    DYKDDDDK (FLAG-tag)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-CuO

    Available since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pB-TR-hSOD1

    Plasmid

    #232478

    Purpose

    Tetracycline inducible PiggyBac vector expressing human SOD1gene including flag-tag (DYKDDDDK) on the 3' end

    Depositor

    Lukas Trantirek

    Article

    Rynes et al Commun Biol. 2025 Feb 7;8(1):194. doi: 1

    Insert

    Homo sapiens superoxide dismutase 1 (SOD1 Human)
    

    Use

    Transposon system

    Tags

    DYKDDDDK (FLAG-tag)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO

    Available since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-T7-ABE8e-SpCas9(ARGIMR)-P2A-EGFP (AHK507)

    Plasmid

    #223118

    Purpose

    pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with ARGIMR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized TadA8e-SpCas9(ARGIMR)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-P2A-EGFP and BPNLS-TadA8e

    Expression

    Mammalian

    Mutation

    TadA8e mutations; nSpCas9(D10A/ARGIMR)=D1135A, S1…

    Promoter

    CMV and T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCMV-T7-ABE8e-SpCas9(LWQYQH)-P2A-EGFP (AHK504)

    Plasmid

    #223119

    Purpose

    pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with LWQYQH amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized TadA8e-SpCas9(LWQYQH)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-P2A-EGFP and BPNLS-TadA8e

    Expression

    Mammalian

    Mutation

    TadA8e mutations; nSpCas9(D10A/LWQYQH)=D1135L, S1…

    Promoter

    CMV and T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV-T7-ABE8e-SpCas9(MWKYQS)-P2A-EGFP (AHK505)

    Plasmid

    #223120

    Purpose

    pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with MWKYQS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized TadA8e-SpCas9(MWKYQS)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-P2A-EGFP and BPNLS-TadA8e

    Expression

    Mammalian

    Mutation

    TadA8e mutations; nSpCas9(D10A/MWKYQS)=D1135M, S1…

    Promoter

    CMV and T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV-T7-ABE8e-SpCas9(MWKYQA)-P2A-EGFP (AHK503)

    Plasmid

    #223122

    Purpose

    pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with MWKYQA amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized TadA8e-SpCas9(MWKYQA)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-P2A-EGFP and BPNLS-TadA8e

    Expression

    Mammalian

    Mutation

    TadA8e mutations; nSpCas9(D10A/MWKYQA)=D1135M, S1…

    Promoter

    CMV and T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV-T7-TadCBEd-SpG-P2A-EGFP (BKS777/AHK571)

    Plasmid

    #223124

    Purpose

    pCMV and pT7 Human expression plasmid for TadCBEd-SpG with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized TadCBEd-SpG-2xUGI-P2A-EGFP
    

    Use

    CRISPR

    Tags

    2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd

    Expression

    Mammalian

    Mutation

    TadA-CDd mutations; nSpG=D10A/D1135L/S1136W/G1218…

    Promoter

    CMV and T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV-T7-TadCBEd-SpRY-P2A-EGFP (BKS428)

    Plasmid

    #223125

    Purpose

    pCMV and pT7 Human expression plasmid for TadCBEd-SpRY with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized TadCBEd-SpRY-2xUGI-P2A-EGFP
    

    Use

    CRISPR

    Tags

    2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd

    Expression

    Mammalian

    Mutation

    TadA-CDd mutations; nSpRY=D10A/A61R/L1111R/D1135L…

    Promoter

    CMV and T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-T7-ABE8e-SpCas9(KWRQLC)-P2A-EGFP (RAS3467)

    Plasmid

    #223112

    Purpose

    pCMV and pT7 Human expression plasmid for ABE8e SpCas9 enzyme with KWRQLC amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized TadA8e-SpCas9(KWRQLC)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-P2A-EGFP and BPNLS-TadA8e

    Expression

    Mammalian

    Mutation

    TadA8e mutations; nSpCas9(D10A/KWRQLC)=D1135K, S1…

    Promoter

    CMV and T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge