169,450 results
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Bacterial Strain#49018PurposeThis is C321.DA with mutS reverted and the lambda prophage removed; it exhibits lesser spontaneous mutagenesis and can be grown at 37 CDepositorBacterial ResistanceBleocin (Zeocin)SpeciesEscherichia coliAvailable SinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only
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p189
Plasmid#65207PurposeMutant EGFP expression plasmid for the creation of a heteroduplex DNA repair assay plasmid with pGEM5Z(+)-EGFP (p111). *Please see notes in comments section.DepositorInsertEGFP
ExpressionBacterial and MammalianMutationPremature stop codon added to pGEM5Z(+)-EGFP (TGG…PromoterCMVAvailable SinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEM5Z(+)-EGFP
Plasmid#65206PurposeEGFP expression plasmid (also called p111) for the creation of a heteroduplex DNA repair assay plasmid with p189. *Please see notes in comments section.DepositorInsertEGFP
ExpressionBacterial and MammalianMutationRestriction enzyme recognition sites added either…PromoterCMVAvailable SinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRBC-sfGFP-150TAG
Plasmid#174076PurposePlasmid with p15a origin of replication expressing codon optimized superfolder GFP-150 TAG with C-terminal His6 tag, under T7 promoter,DepositorInsertSuperfolder GFP
TagsHis-6ExpressionBacterialMutationN150TAGPromoterT7Available SinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPGC1a_Distal Promoter-FLuc2P
Plasmid#154260PurposeDestabilized firefly luciferase reporter containing the upstream Human PGC-1alpha Distal Promoter from -500 to +52.DepositorInsertPGC-1a Distal Promoter (PPARGC1A Human)
ExpressionMammalianPromoterHuman PGC-1alpha Distal PromoterAvailable SinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
TRUPATH Triple Gaq
Plasmid#196058PurposeEncodes a G alpha subunit (GNAQ) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteinsDepositorUseLuciferaseTagsGFP2 and GSAG linkerExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable SinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKM464 (deletions)
Plasmid#108322PurposeOrbit integrating plasmid to delete target gene.DepositorInsertBxb1 attB site
UseOrbit integration plasmid for creating knockouts …Available SinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28-hXRCC1
Plasmid#70759Purposebacterial expression of human XRCC1DepositorAvailable SinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-GCaMP5G
Plasmid#31788Purposea single-wavelength GCaMP3-based calcium indicator with improved response. Please also see the GCaMP6s/m/f indicators.DepositorInsertGCaMP5G
Tags6xHis, T7 epitope, and Xpress tagExpressionMammalianPromoterCMVAvailable SinceAug. 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-C1-Nir1-LNS2
Plasmid#220084Purposehigh affinity PA biosensor with red fluorophore (also known as mCherry-PILS-Nir1)DepositorInsertPITPNM3 (PITPNM3 Human)
TagsmCherry-GGSGGMExpressionMammalianMutationamino acids 613-897PromoterCMVAvailable SinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only