We narrowed to 15,991 results for: LEA

1. pT7-7 asyn G51D

   Plasmid

   #105747

   Purpose

   Bacterial expression of mutant human alpha synuclein

   Depositor

   Hilal Lashuel

   Article

   Fares et al Hum Mol Genet. 2014 Sep 1;23(17):4491-50

   Insert

   alpha-synuclein G51D (SNCA Human)
   

   Expression

   Bacterial

   Mutation

   G51D

   Promoter

   T7

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. kiCAP-AAV-MyoAAV2A

   Plasmid

   #224440

   Purpose

   Rep/Cap plasmid for the production of MyoAAV 2A, a muscle-tropic AAV capsid in mice.

   Depositor

   Pardis Sabeti

   Article

   Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

   Insert

   AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
   

   Use

   AAV

   Mutation

   RGDQTTL insert between amino acids 588 and 589 of…

   Promoter

   p41

   Available Since

   Sept. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-EF1a-DIO-FLAG-KORD-P2A-ArgiNLS-AausFP1

   Plasmid

   #220612

   Purpose

   Cre-dependent co-expression of FLAG-tagged inhibitory KORD DREADD receptor, and a single-cell discriminating version of AausFP1 as a reporter.

   Depositor

   Sam Golden

   Article

   Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

   Insert

   FLAG-KORD-P2A-ArgiNLS-AausFP1
   

   Use

   AAV and Cre/Lox

   Tags

   FLAG; ArgiNLS

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. kiCAP-AAV-MyoAAV4A

   Plasmid

   #224448

   Purpose

   Rep/Cap plasmid for the production of MyoAAV 4A, a muscle-tropic AAV capsid in mice and macaques.

   Depositor

   Pardis Sabeti

   Article

   Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

   Insert

   AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
   

   Use

   AAV

   Mutation

   RGDYNSL insert between amino acids 588 and 589 of…

   Promoter

   p41

   Available Since

   Sept. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. kiCAP-AAV-MyoAAV1A

   Plasmid

   #224437

   Purpose

   Rep/Cap plasmid for the production of MyoAAV 1A, a muscle-tropic AAV capsid in mice.

   Depositor

   Pardis Sabeti

   Article

   Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

   Insert

   AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
   

   Use

   AAV

   Mutation

   RGDLTTP insert between amino acids 588 and 589 of…

   Promoter

   p41

   Available Since

   Sept. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. N-Terminal Split Cas9 with GyrA intein

   Plasmid

   #58693

   Purpose

   Expresses truncated N-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 production

   Depositor

   Gang Bao

   Article

   Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103

   Insert

   Humanized N-Terminal S. pyogenes Cas9 with GyrA Nsplit Intein
   

   Use

   AAV and CRISPR

   Tags

   3xFlag, GyrA Nsplit Intein, and NLS

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   July 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. lentiCRISPR v2-Nr4a1 double gRNAs

   Plasmid

   #162791

   Purpose

   Multiplex Guide RNAs to generate Nr4a1 knockout by CRISPR

   Depositor

   Matthew Steinhauser

   Article

   Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

   Insert

   Multiplex Nr4a1 gRNA 1 and 2 (Nr4a1 Mouse)
   

   Use

   CRISPR and Lentiviral

   Promoter

   hU6

   Available Since

   Dec. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hSyn-DIO-FLAG-KORD-P2A-ArgiNLS-AausFP1

   Plasmid

   #220610

   Purpose

   Neuron-specific, Cre-dependent co-expression of FLAG-tagged inhibitory KORD DREADD receptor, and a single-cell discriminating version of AausFP1 as a reporter

   Depositor

   Sam Golden

   Article

   Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

   Insert

   FLAG-KORD-P2A-ArgiNLS-AausFP1
   

   Use

   AAV and Cre/Lox

   Tags

   FLAG; ArgiNLS

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-hSyn-DIO-FLAG-KORD-P2A-3xHA-hM3D(Gq)

   Plasmid

   #220611

   Purpose

   Neuron-specific, Cre-dependent co-expression of FLAG-tagged inhibitory KORD DREADD receptor, and 3x HA-tagged excitatory hM3D(Gq) DREADD receptor.

   Depositor

   Sam Golden

   Article

   Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

   Insert

   FLAG-KORD-P2A-3x HA-hM3D(Gq)
   

   Use

   AAV and Cre/Lox

   Tags

   FLAG; ArgiNLS

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   Aug. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. GFP-ING2(pHD)

    Plasmid

    #21589

    Depositor

    Junying Yuan

    Article

    Gozani et al Cell. 2003 Jul 11. 114(1):99-111.

    Insert

    GFP-ING2(pHD) (Ing2 Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    contains Ing2 plant homeodomain (PHD) amino acids…

    Available Since

    July 28, 2009

    Availability

    Academic Institutions and Nonprofits only

11. pINDUCER20-Tau

    Plasmid

    #92201

    Purpose

    Retroviral overexpression vector (doxycycline-inducible) for Tau

    Depositor

    Huda Zoghbi

    Article

    Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

    Insert

    MAPT (MAPT Human)
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mammalian

    Available Since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCR2.1 Clover-LMNA Donor

    Plasmid

    #122508

    Purpose

    Homology repair template for in frame (first exon) clover knock-in of human LMNA gene

    Depositor

    Graham Dellaire

    Article

    Pinder et al Nucleic Acids Res. 2015 Oct 30;43(19):93

    Insert

    Homology Repair Template for Human LMNA (N-Terminal Clover Tag) (LMNA Human)
    

    Use

    Homology repair template plasmid (donor plasmid)

    Tags

    Clover

    Available Since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDY0663 ACTB atgRNA with v2 scaffold

    Plasmid

    #179108

    Purpose

    ACTB N-term PBS 13 RT 29 Bxb1 AttB 46 atgRNA with v2 scaffold

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

    Insert

    ACTB N-term PBS 13 RT 29 AttB 46 atgRNA with v2 scaffold
    

    Use

    CRISPR

    Available Since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-EF1a-DIO-FLAG-hM4D(Gi)-P2A-ArgiNLS-AausFP1

    Plasmid

    #220613

    Purpose

    Cre-dependent co-expression of FLAG-tagged inhibitory hM4D(Gi) DREADD receptor, and a single-cell discriminating version of AausFP1 as a reporter.

    Depositor

    Sam Golden

    Article

    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

    Insert

    FLAG-hM4D(Gi)-P2A-ArgiNLS-AausFP1
    

    Use

    AAV and Cre/Lox

    Tags

    FLAG; ArgiNLS

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

15. pAAV-EF1a-DIO-3xHA-hM3D(Gq)-P2A-ArgiNLS-oScarlet

    Plasmid

    #220614

    Purpose

    Cre-dependent co-expression of 3x HA-tagged excitatory hM3D(Gq) DREADD receptor, and a single-cell discriminating version of oScarlet as a reporter.

    Depositor

    Sam Golden

    Article

    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

    Insert

    3x HA-hM3D(Gq)-P2A-AgiNLS-oScarlet
    

    Use

    AAV and Cre/Lox

    Tags

    3x HA; ArgiNLS

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMSCV40-EF1a-hBCL10-SO

    Plasmid

    #75256

    Purpose

    retroviral expression vector for expression of StrepOne-Tagged human BCL10

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    Bcl10 (BCL10 Human)
    

    Use

    Retroviral

    Tags

    StrepOne Tag

    Expression

    Mammalian

    Promoter

    pMSCV-LTRs, EF1a

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAVS1-Neo-M2rtTA

    Plasmid

    #60843

    Purpose

    AAVS1 donor vector for genomic targeting

    Depositor

    Rudolf Jaenisch

    Article

    DeKelver et al Genome Res. 2010 Aug;20(8):1133-42. doi:

    Inserts

    M2rtTA
    Neo
    

    Use

    Targeting donor

    Available Since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAVS1-eTLR

    Plasmid

    #214737

    Purpose

    encodes the enhanced traffic light reporter (eTLR) flanked by homology arms for integration into the AAVS1 safe harbor locus

    Depositor

    Gil Westmeyer

    Article

    Truong et al Nat Methods. 2024 Feb 1. doi: 10.1038/s4

    Insert

    AAVS1-eTLR (PPP1R12C Chicken, CMV, Anguilla japonica, influenza, Rabbit, Synthetic, Bovine, Human)
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Available Since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDY0209 ACTB Nicking guide +48

    Plasmid

    #179109

    Purpose

    ACTB N-term Nicking guide 1 +48 guide

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

    Insert

    ACTB N-term Nicking guide 1 +48 guide
    

    Use

    CRISPR

    Available Since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMSCV-FLAG-hIKAROS-IRES-GFP

    Plasmid

    #74046

    Purpose

    retroviral plasmid for expression of FLAG-tagged human Ikaros corresponding to cDNA from XM_011515058.1

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    human Ikaros (IKZF1) (IKZF1 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    pMSCV-LTRs

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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