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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 20,549 results for: LEA

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Showing: 7641 - 7660 of 20549 results

  2. nLuc_AP10_PPVs_P9mS

    Plasmid
    #119302
    Purpose
    Expresses N-terminus of split luciferase fused to antiparallel coiled-coil AP10 connected with autoinhibitory coil P9mS and PPVs cleavage site in antiparallel harpin linker/for SPOC logic
    Depositor
    Roman Jerala
    Publication
    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    split nLuc fused to antiparallel Coiled-coil AP10 connected with P9mS via PPV cleavable linker
    Use
    Luciferase
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    119302-plasmid-map-sequence-id-232037

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  7. pET24a-VHH-myc

    Plasmid
    #109422
    Purpose
    Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to a myc epitope. VHH-myc also contains a T7, HA, BAP and His6 epitope
    Depositor
    Martin Spiess
    Publication
    Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115
    Insert
    anti-GFP nanobody fused to a T7, myc, HA, BAP and His6 epitope
    Tags
    BAP, HA, His6, T7, and myc
    Expression
    Bacterial
    Promoter
    T7
    Available since
    June 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    109422-plasmid-map-sequence-id-211585

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  8. pNGVL4a-hCRTmE6mE7mL2

    Plasmid
    #104153
    Purpose
    Expresses human calreticulin fused to MmuPV1 E6, MmuPV1 E7 and MmuPV1 L2 residues 11-210 in mammalian cells
    Depositor
    Richard Roden
    Publication
    Jiang et al J Virol. 2017 Jul 12;91(15). pii: e00699
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hCRTmE6mE7mL2 11-210 (CALR, E6, E7, L2 MmuPV1, Human)
    Expression
    Mammalian
    Mutation
    residues 11 to 200 of the late protein L2
    Promoter
    CMV
    Available since
    Dec. 18, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    104153-plasmid-map-sequence-id-200449

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  9. pRSET a sfGCaMP6s-T78H

    Plasmid
    #100023
    Purpose
    Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. cpEGFP replaced with a superfolder cpGFP variant containing T78H mutation
    Depositor
    Wolf Frommer
    Publication
    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10
    Insert
    sfGCaMP6s-T78H
    Tags
    6x HIS tag and Xpress_EK tag
    Expression
    Bacterial
    Mutation
    cpEGFP replaced with superfolder cpGFP and T78H m…
    Promoter
    T7
    Available since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    100023-plasmid-map-sequence-id-194290

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  11. pCMV-hAR-CA576-Z

    Plasmid
    #89108
    Purpose
    mammalian expression of full-length human androgen receptor with DNA binding domain mutation CA576
    Depositor
    Elizabeth Wilson
    Publication
    Zhou et al J Biol Chem. 1994 May 6;269(18):13115-23
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hAR-C576A (AR Human)
    Expression
    Mammalian
    Mutation
    C576A
    Promoter
    CMV
    Available since
    April 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  12. pMT-flk1-cytoBirA-2A-mCherry_Ras

    Plasmid
    #80056
    Purpose
    Flk1/Kdrl promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Publication
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    Tags
    3x HA
    Expression
    Bacterial
    Promoter
    flk1
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80056-plasmid-map-sequence-id-151372

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  19. pNJB91

    Plasmid
    #51519
    Purpose
    Gateway entry vector with attL1 and attR5 sites. Between the att sites are two FokI coding sequences transcriptionally linked by a T2A sequence. There is a nos-T after the last FokI.
    Depositor
    Daniel Voytas
    Publication
    Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:
    Type
    Empty backbone
    Use
    Gateway Cloning
    Available since
    March 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    51519-plasmid-map-sequence-id-78693

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Showing: 7641 - 7660 of 20549 results