We narrowed to 19,051 results for: Tec

1. pVH663

   Plasmid

   #169607

   Purpose

   Tier-2 vector encoding PTetO7-driven SEAP-p2A-iRFP670, PmPGK1-driven tTA and PmPGK1-driven YPet expression (PTetO7-CMVmin-2-SEAP-p2A-iRFP670-pA::PmPGK1-tTA-pA::PmPGK1-YPet-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   tetracycline-controlled SEAP and iRFP expression, PPGK-driven tTA expression cassette and PPGK-driven YPet expression cassette
   

   Expression

   Mammalian

   Promoter

   tetO7-PCMVmin / PPGK / PPGK

   Available Since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pVH19-Tier1-PhCMV-p300core

   Plasmid

   #169572

   Purpose

   Tier-1 vector encoding PhCMV-driven histone acetyltransferase p300 core domain expression (PhCMV-p300core-pA)

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven P300core (EP300 Human)
   

   Expression

   Mammalian

   Promoter

   PhCMV

   Available Since

   June 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTS2304-Tier1-Tactb-PPGK-NLuc_TB

   Plasmid

   #169549

   Purpose

   Tier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' Tactb insulator sequence (Tactb-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   Tactb-modulated PPGK-driven NanoLuc Luciferase - mTagBFP expression cassette
   

   Expression

   Mammalian

   Promoter

   PPGK

   Available Since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTS1046-Tier1-PhCMV-4xCox8-mRuby2

   Plasmid

   #169537

   Purpose

   Tier-1 vector encoding PhCMV-driven mRuby2 localized to the mitochondrial matrix via a four-fold Cox8 localization signal (PhCMV-4xCox8-mRuby2-pA)

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven miochondria-localized monomeric red fluorescent protein mRuby2
   

   Expression

   Mammalian

   Promoter

   PhCMV

   Available Since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTS2380-Tier1-PhCMV-TM

   Plasmid

   #169485

   Purpose

   Tier-1 vector encoding PhCMV-driven transmembrane domain from Junctophilin-1 (TM) (PhCMV-TM-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven Junctophilin 2 transmembrane domain (JPH2 Human)
   

   Expression

   Mammalian

   Promoter

   PhCMV

   Available Since

   June 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. 1098E=TI-pgSIT[tra,bTub,Hasp70Bb-Cas9]

   Plasmid

   #149427

   Purpose

   The attB plasmid with the mini-white harboring two U6.3-gRNAs targeting Dmel tra and bTub genes, and Hsp70Bb-Cas9-T2A-eGFP-p10.

   Depositor

   Omar Akbari

   Article

   Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

   Inserts

   U6.3-gRNAs[TraB, bTub]
   Hsp70Bb-Cas9-T2A-eGFP-p10
   

   Use

   CRISPR

   Tags

   eGFP

   Expression

   Insect

   Available Since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. PdnaK-IR2-IR2 GFP pZa

   Plasmid

   #170083

   Purpose

   GFP expression under the control of E. coli dnaK promoter engineered with double IR2 HAIR motif from M. tuberculosis

   Depositor

   Urartu Seker

   Article

   Saltepe et al ACS Synth Biol. 2019 Oct 18;8(10):2404-2

   Insert

   Green fluorescent protein
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   PdnaK-IR2-IR2

   Available Since

   Sept. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYPQ265C

   Plasmid

   #164715

   Purpose

   Gateway entry clone (attL1 & attR5) hAID-CBE_V01 for C-T base editing

   Depositor

   Yiping Qi

   Article

   Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

   Insert

   hAID-zCas9(D10A)-UGI
   

   Use

   CRISPR; Gateway compatible haid-cbe_v01 entry clo…

   Expression

   Plant

   Available Since

   May 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYPQ265E1

   Plasmid

   #164718

   Purpose

   Gateway entry clone (attL1 & attR5) A3A-CBE_V01 for C-T base editing

   Depositor

   Yiping Qi

   Article

   Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

   Insert

   A3A-zCas9(D10A)-UGI
   

   Use

   CRISPR; Gateway compatible a3a-cbe_v01 entry clone

   Expression

   Plant

   Available Since

   May 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYPQ265E3

    Plasmid

    #164720

    Purpose

    Gateway entry clone (attL1 & attR5) eA3A-CBE_V01 for C-T base editing

    Depositor

    Yiping Qi

    Article

    Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

    Insert

    eA3A-zCas9(D10A)-UGI
    

    Use

    CRISPR; Gateway compatible ea3a-cbe_v01 entry clo…

    Expression

    Plant

    Available Since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUPD2 AtU6-26:AsCas12a_DR (GB1442)

    Plasmid

    #160574

    Purpose

    AsCas12a direct repeat sequence fused to the A. thaliana U6-26 promoter used for the assembly of gRNA expression cassettes.

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

    Insert

    AtU6-26:AsCas12a_DR
    

    Use

    CRISPR and Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUPD2 LbCas12a (GB1439)

    Plasmid

    #160573

    Purpose

    Human codon optimized coding sequence of Cas12a from Lachnospiraceae CRISPR system (LbCas12a).

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

    Insert

    LbCas12a
    

    Use

    CRISPR and Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUPD2 AsCas12a (GB1438)

    Plasmid

    #160572

    Purpose

    Human codon optimized coding sequence of Cas12a from Acidominococcus sp (AsCas12a).

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

    Insert

    AsCas12a
    

    Use

    CRISPR and Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. KG#797

    Plasmid

    #110916

    Purpose

    Expresses the active zone - enriched protein Sentryn-GFP in a subset of cholinergic ventral nerve cord motor neurons

    Depositor

    Kenneth Miller

    Article

    Edwards et al Genetics. 2018 Nov;210(3):947-968. doi:

    Inserts

    unc-129 promoter
    STRN-1 (Sentryn)-GFP
    unc-54 3' control region with 1 intron just upstream and 1 intron in control region
    

    Tags

    GFP (contains 3 artificial introns)

    Expression

    Bacterial

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUE1-TSP-ORF68 K174A/R179A/K182A

    Plasmid

    #162654

    Purpose

    Expresses ORF68 K174A/R179A/K182A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    ORF68
    

    Tags

    N-terminal 2xStrep-Tag II and HRV 3C site

    Expression

    Mammalian

    Mutation

    Amino acids K174, R179, and K182 mutated to alani…

    Promoter

    CMV

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. GSX2-P2A-tGFP-DTA

    Plasmid

    #161750

    Purpose

    tGFP plasmid for the c-terminal targeting of human GSX2 locus

    Depositor

    Elena Cattaneo

    Article

    Besusso et al Stem Cell Res. 2020 Dec;49:102016. doi:

    Insert

    turboGFP
    

    Use

    CRISPR

    Tags

    P2A-tGFP

    Expression

    Mammalian

    Promoter

    endogenous GSX2 promoter

    Available Since

    Dec. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-T7-AcrIIA1-NLS(SV40) (KAC478)

    Plasmid

    #133795

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized AcrIIA1 with C-terminal NLS (SV40)

    Depositor

    Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Osuna et al Cell Host Microbe. 2020 Apr 18. pii: S19

    Insert

    human codon optimized AcrIIA1
    

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    CMV and T7

    Available Since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-T7-AcrIIA1(T16A)-NLS(SV40) (KAC511)

    Plasmid

    #133797

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized AcrIIA1(T16A) with C-terminal NLS (SV40)

    Depositor

    Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Osuna et al Cell Host Microbe. 2020 Apr 18. pii: S19

    Insert

    human codon optimized AcrIIA1(T16A)
    

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Mutation

    T16A

    Promoter

    CMV and T7

    Available Since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-T7-AcrIIA1-Leu-NLS(SV40) (KAC440)

    Plasmid

    #133799

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized Leu-AcrIIA1 with C-terminal NLS (SV40)

    Depositor

    Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Osuna et al Cell Host Microbe. 2020 Apr 18. pii: S19

    Insert

    human codon optimized Leu-AcrIIA1
    

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    CMV and T7

    Available Since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-T7-AcrIIA1-Eriv-NLS(SV40) (KAC442)

    Plasmid

    #133800

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized Eriv-AcrIIA1 with C-terminal NLS (SV40)

    Depositor

    Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Osuna et al Cell Host Microbe. 2020 Apr 18. pii: S19

    Insert

    human codon optimized Eriv-AcrIIA1
    

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    CMV and T7

    Available Since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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