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pDEST-ORF-V2

Plasmid

#73638

Purpose

Gateway™ destination vector encoding C-terminal fusion with Venus fluorescent protein fragment 2 (V2).

Depositor

Darren Saunders

Article

Croucher et al Sci Signal. 2016 Jul 12;9(436):ra69. doi

Type

Empty backbone

Tags

Venus fragment 2 (V2)

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2016

Availability

Academic Institutions and Nonprofits only

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pCSIIhyg-rtTA

Plasmid

#139480

Purpose

Expresses rtTA, Tet-ON reverse tetracycline-controlled trans-activator in mammalian cells.

Depositor

Kazuhiro Aoki

Article

Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2

Insert

rtTA, Tet-ON reverse transactivator

Use

Lentiviral

Expression

Mammalian

Promoter

Human EF1a promoter

Available Since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

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plRL118

Plasmid

#163632

Purpose

Renilla luciferase reporter plasmid; Giles integrating.

Depositor

Jeremy Rock

Article

Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

Type

Empty backbone

Use

Luciferase

Expression

Bacterial

Promoter

Mycobacterial Optimized Promoter (MOP)

Available Since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCS6-YFP-SMASh

Plasmid

#68853

Purpose

Expresses SMASh tag fused to YFP at C terminus in mammalian cells

Depositor

Michael Lin

Article

Chung et al Nat Chem Biol. 2015 Jul 27. doi: 10.1038

Insert

YFP-SMASh

Tags

SMASh tag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pDEST-ORF-V1

Plasmid

#73637

Purpose

Gateway™ destination vector encoding C-terminal fusion with Venus fluorescent protein fragment 1 (V1)

Depositor

Darren Saunders

Article

Croucher et al Sci Signal. 2016 Jul 12;9(436):ra69. doi

Type

Empty backbone

Tags

Venus fragment 1 (V1)

Expression

Mammalian

Promoter

CMV

Available Since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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YIplac211-NHX1-HaloTagx2

Plasmid

#177107

Purpose

Expresses Nhx1-HaloTagx2 in yeast cells

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

Insert

NHX1 (NHX1 Budding Yeast)

Expression

Yeast

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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Gg 3kb Green opsin GFP

Plasmid

#72919

Purpose

green opsin promoter from chicken (Gallus gallus) gDNA chr26:4,504,913–4,501,931 in galGal4 driving GFP

Depositor

Joseph Corbo

Article

Enright et al J Comp Neurol. 2015 Mar 1;523(4):649-68.

Insert

chicken green opsin promoter

Available Since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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AsdCas12a KRAB

Plasmid

#188502

Purpose

Expresses FLAG tagged AsdCas12a KRAB

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas12a

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D908A

Promoter

EF1a

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-U6-epegRNA eBAR1-SV40-puro

Plasmid

#240535

Purpose

Lentiviral eBAR1 backbone plasmid to insert epegRNAs (tevopreq1) of interest with puromycin resistance. The cloning site is BsmBI.

Depositor

Wensheng Wei

Article

Niu et al Nat Biotechnol. 2025 Jun 24. doi: 10.103

Insert

epegRNA-eBAR1

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Beclin1

Plasmid

#21150

Depositor

Junying Yuan

Article

Shibata et al J Biol Chem. 2006 May 19. 281(20):14474-

Insert

PcDNA3-Beclin1 (BECN1 Human)

Expression

Mammalian

Available Since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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YIplac211-NHX1-iGFP

Plasmid

#177108

Purpose

Expresses Nhx1-iGFP in yeast cells

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

Insert

NHX1 (NHX1 Budding Yeast)

Expression

Yeast

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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Salsa-BC1-P1A1

Plasmid

#202842

Purpose

Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC1

Depositor

Noah Palm

Article

Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

Insert

EGFP/BC1

Expression

Mammalian

Promoter

Tet Response Element (TRE)

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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UAS:Zebrabow-V

Plasmid

#118218

Purpose

For broad or variegated gal4 inducible expression of Brainbow in zebrafish

Depositor

Albert Pan, Alex Schier

Article

Pan et al Development. 2013 Jul;140(13):2835-46. d

Insert

Brainbow 1.0 L

Use

Zebrafish expression

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pCI pHluorin Syt12

Plasmid

#184513

Purpose

Mammalian expression of Synaptotagmin 12 with pH-sensitive fluorescent protein

Depositor

Edwin Chapman

Article

Dean et al Mol Biol Cell. 2012 May;23(9):1715-27. d

Insert

Synaptotagmin 12 (Syt12 Rat)

Tags

Super Ecliptic pHluorin

Expression

Mammalian

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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LTR5HS S. aureus scaffold CARGO array

Plasmid

#191317

Purpose

Contains an array of guide RNAs targeting LTR5Hs elements

Depositor

Joanna Wysocka

Article

Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

Insert

Array of guide RNAs

Use

CRISPR

Tags

mCherry

Expression

Mammalian

Available Since

Nov. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pRSF_AaRbcLS

Plasmid

#229505

Purpose

Expresses Anthoceros agrestis Rubisco large and small subunit (C terminus hexahistidine)

Depositor

Laura Gunn

Article

Oh et al Mol Plant. 2024 Dec 2;17(12):1833-1849.

Inserts

Rubisco large subunit
Rubisco small subunit

Tags

hexahistidine

Expression

Bacterial

Mutation

N terminus chloroplast transit peptide truncation

Promoter

T7

Available Since

June 20, 2025

Availability

Academic Institutions and Nonprofits only

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pCIP-AMPKa1_KD

Plasmid

#79011

Purpose

For mammalian expression of a kinase dead (K47R) version of AMPK alpha1.

Depositor

Reuben Shaw

Article

Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

Insert

AMPK alpha 1 (PRKAA1 Human)

Expression

Mammalian

Mutation

K47R

Promoter

pCAGGs

Available Since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-ChrimsonR-mRuby2-KV2.1-WPRE-SV40

Plasmid

#124603

Purpose

Cre-dependent soma-localized ChrimsonR for optogenetic stimulation

Depositor

Christopher Harvey

Article

Chettih et al Nature. 2019 Mar 6. pii: 10.1038/s41586-

Has Service

AAV9

Insert

Soma-localized ChrimsonR

Use

AAV

Tags

mRuby2

Expression

Mammalian

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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SaCas9

Plasmid

#188494

Purpose

Expresses FLAG tagged SaCas9

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

Cas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Gα13*-BERKY3

Plasmid

#158218

Purpose

BRET biosensor for detection of Gα13-GTP in mammalian expression vector pcDNA3.1(+)

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

Lyn11-Nluc-ER/K linker-3xYFP-PRG(RH)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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