We narrowed to 10,082 results for: crispr plasmids

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pFB-U6-Chrna2-gRNA-hSyn-mCherry-WPRE-SV40pA

Plasmid

#128337

Purpose

pAAV encoding gRNA sequence for loss-of-function indels

Depositor

Ryan Drenan

Article

Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

Insert

Chrna2 (Chrna2 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

July 19, 2019

Availability

Academic Institutions and Nonprofits only

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pFB-U6-Chrna4-gRNA-hSyn-mCherry-WPRE-SV40pA

Plasmid

#128339

Purpose

pAAV encoding gRNA sequence for loss-of-function indels

Depositor

Ryan Drenan

Article

Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

Insert

Chrna4 (Chrna4 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

July 19, 2019

Availability

Academic Institutions and Nonprofits only

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U6_sgRNA_CAG_hSt1Cas9_CNRZ1066

Plasmid

#136651

Purpose

A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD9:CNRZ1066 chimera) and its U6-driven sgRNA.

Depositor

Yannick Doyon

Article

Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

Inserts

St1Cas9 LMD-9:CNRZ_1066 Chimera
sgRNA for St1Cas9

Use

CRISPR and Synthetic Biology

Tags

SV40 NLS

Expression

Mammalian

Promoter

CAG and hU6

Available Since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pFB-U6-Chrnb3-gRNA-hSyn-mCherry-WPRE-SV40pA

Plasmid

#128344

Purpose

pAAV encoding gRNA sequence for loss-of-function indels

Depositor

Ryan Drenan

Article

Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

Insert

Chrnb3 (Chrnb3 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

July 19, 2019

Availability

Academic Institutions and Nonprofits only

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pLL6 sgRNA(MS2)-MS2-hA3a D131E

Plasmid

#112132

Purpose

empty sgRNA cloning vector with MS2-humanA3a D131E

Depositor

Fei-Long Meng

Article

Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

Insert

human Apobec3a D131E mutant (APOBEC3A Human)

Use

CRISPR

Expression

Mammalian

Mutation

D131E mutant in hA3a

Promoter

hU6,CBh

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pGEM_H1.2_HaloTag

Plasmid

#247481

Purpose

CRISPR/Cas9 donor plasmid to tag human histone H1.2 with Halo tag

Depositor

Kazuhiro Maeshima

Article

Shimazoe et al Sci Adv. 2026 Apr 10;12(15):eaec9801. do

Insert

H1.2 (HIST1H1C) (H1-2 Human)

Use

CRISPR

Tags

HaloTag

Available Since

April 13, 2026

Availability

Academic Institutions and Nonprofits only

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pGEM_H1.0_HaloTag

Plasmid

#247483

Purpose

CRISPR/Cas9 donor plasmid to tag human histone H1.0 with Halo tag

Depositor

Kazuhiro Maeshima

Article

Shimazoe et al Sci Adv. 2026 Apr 10;12(15):eaec9801. do

Insert

H1.0 (H1F0) (H1-0 Human)

Use

CRISPR

Tags

HaloTag

Available Since

April 13, 2026

Availability

Academic Institutions and Nonprofits only

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pGEM_H1.2_mAID_mClover

Plasmid

#247486

Purpose

CRISPR/Cas9 donor plasmid to tag human histone H1.2 with mAID-mClover

Depositor

Kazuhiro Maeshima

Article

Shimazoe et al Sci Adv. 2026 Apr 10;12(15):eaec9801. do

Insert

H1.2 (HIST1H1C) (H1-2 Human)

Use

CRISPR

Tags

mAID and mClover

Available Since

April 13, 2026

Availability

Academic Institutions and Nonprofits only

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Suntag_dPRDM9_FWA_g4

Plasmid

#249562

Purpose

dCas9_dPRDM9 catalytically dead version

Depositor

Jake Harris

Article

Binenbaum et al Nat Commun. 2025 Oct 31;16(1):9587. doi:

Insert

PRDM9 (Prdm9 Mouse)

Use

Synthetic Biology

Tags

HA and sfGFP

Expression

Plant

Mutation

G282A (Numbering from the the full length sequenc…

Promoter

UBQ10

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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pDonor RUNX3-P2A-tdTomato

Plasmid

#246655

Purpose

Donor plasmid for Crispr/Cas9 gene editing of the human RUNX3 locus

Depositor

Joriene de Nooij

Article

Malka-Gibor et al Stem Cell Reports. 2026 Jan 2:102753. do

Insert

P2A-tdTomato flanked by RUNX3 homology regions (RUNX3 Synthetic, Human)

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pDonor NTRK1-P2A-tdTomato

Plasmid

#246653

Purpose

Donor plasmid for Crispr/Cas9 gene editing of the human NTRK1 locus

Depositor

Joriene de Nooij

Article

Malka-Gibor et al Stem Cell Reports. 2026 Jan 2:102753. do

Insert

P2A-tdTomato flanked by NTRK1 homology regions (NTRK1 Synthetic, Human)

Use

CRISPR

Expression

Mammalian

Mutation

tdTomato was altered to contain a silent SAL1 res…

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pDS48

Plasmid

#241839

Purpose

Cas9-gRNA construct targeting the UBE3A locus

Depositor

Albert Keung

Article

Kelkar et al Front Cell Dev Biol. 2025 Aug 29;13:1665

Insert

Cas9-gRNA (UBE3A )

Use

AAV and CRISPR

Expression

Mammalian

Available Since

Sept. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pSCBE3-HF1

Plasmid

#218156

Purpose

This plasmid harbors the base editor SCBE3-HF1 along with an sgRNA cloning cassette, facilitating high-fidelity cytosine base editing in Streptomyces.

Depositor

Yuhui Sun

Article

Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

Inserts

scbe3-HF1
a programmable sgRNA cloning cassette

Use

CRISPR; Sgrna transcription

Expression

Bacterial

Mutation

Mutations in the portion of SpCas9 : D10A / N497A…

Promoter

rpsL promoter

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pSCBE3-Hypa

Plasmid

#218157

Purpose

This plasmid harbors the base editor SCBE3-Hypa along with an sgRNA cloning cassette, facilitating high-fidelity cytosine base editing in Streptomyces.

Depositor

Yuhui Sun

Article

Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

Inserts

scbe3-Hypa
a programmable sgRNA cloning cassette

Use

CRISPR; Sgrna transcription

Expression

Bacterial

Mutation

Mutations in the portion of SpCas9 : D10A / N692A…

Promoter

rpsL promoter

Available Since

May 10, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS-HZ08-pAAVsc.U6-tracrRNA

Plasmid

#211816

Purpose

AAV vector expressing tracrRNA under U6 promoter

Depositor

Erik Sontheimer

Article

Zhang et al Nucleic Acids Res. 2023 Nov 30:gkad1125.

Insert

U6-tracrRNA

Use

AAV

Expression

Mammalian

Promoter

U6

Available Since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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B-HeFSpCas9-A695Q

Plasmid

#207368

Purpose

Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-HeFSpCas9-A695Q

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

N497A, R661A, K848A, Q926A, K1003A, R1060A, amino…

Promoter

CBh

Available Since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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B-HeFSpCas9-A926Q

Plasmid

#207370

Purpose

Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-HeFSpCas9-A926Q

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

N497A, R661A, Q695A, K848A, K1003A, R1060A, amino…

Promoter

CBh

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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B-evoSpCas9-E526K

Plasmid

#207365

Purpose

Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-evoSpCas9-E526K

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

M495V, Y515N, R661Q, amino acids 1005-1013 replac…

Promoter

CBh

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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B-evoSpCas9-V495M

Plasmid

#207363

Purpose

Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-evoSpCas9-V495M

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Y515N, K526E, R661Q, amino acids 1005-1013 replac…

Promoter

CBh

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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B-HeFSpCas9-A1060R

Plasmid

#207372

Purpose

Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-HeFSpCas9-A1060R

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

N497A, R661A, Q695A, K848A, Q926A, K1003A, amino …

Promoter

CBh

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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