We narrowed to 2,757 results for: fas

1. mAzurite-16xRhoBAST

   Plasmid

   #196347

   Purpose

   Expression of mAzurite mRNA with 16xRhoBAST in 3'UTR

   Depositor

   Murat Sunbul

   Article

   Englert et al Nat Commun. 2023 Jun 30;14(1):3879. doi:

   Insert

   mAzurite-16xRhoBAST
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pmScarlet3-Peroxi_C1

   Plasmid

   #189769

   Purpose

   Construct for labelling peroxisomes in mammalian cells with peroxisomal targeted mScarlet3

   Depositor

   Dorus Gadella

   Article

   Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

   Insert

   mScarlet3-Peroxi
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET-SisTnpB1–ωRNA

   Plasmid

   #228365

   Purpose

   Expresses SisTnpB1 and RNP complex in E. coli Rosetta (DE3) cells

   Depositor

   Tao Liu

   Article

   Xu et al Commun Biol. 2024 Nov 20;7(1):1541. doi:

   Insert

   SisTnpB1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDule-Tet3.0

   Plasmid

   #174080

   Purpose

   Tetrazine 3.0 tRNA synthetase and cognate amber suppressing tRNA derived from M. barkeri for expression in E.coli.

   Depositor

   Ryan Mehl

   Article

   Jang et al J Am Chem Soc. 2020 Apr 22;142(16):7245-

   Insert

   Tet3.0 tRNA synthetase and tRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   L270G, Y271L, N311G, C313A

   Promoter

   Available since

   Jan. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Flag-FOXH1

   Plasmid

   #153125

   Purpose

   Expression of Flag tagged human FOXH1 in mammalian cells

   Depositor

   Stefan Koch

   Article

   Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

   Insert

   human FOXH1 (FOXH1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE94

   Available since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBad-LgBiT-PhoCl1-SmBiT-MBP

   Plasmid

   #164034

   Purpose

   luciferase-based PhoCl screening construct

   Depositor

   Robert Campbell

   Article

   Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

   Insert

   LgBiT-PhoCl1-SmBiT-MBP
   

   Use

   Tags

   His tag, T7 tag, Xpress Tag

   Expression

   Bacterial

   Mutation

   Promoter

   araBad promotor

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCoofy57

   Plasmid

   #122009

   Purpose

   Mammalian vector for parallel SLIC cloning containing the VEGF signal sequence for secretion, N-terminal His, PreScission Cleavage site and different C-tag options

   Depositor

   Sabine Suppmann

   Article

   Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

   Type

   Empty backbone

   Use

   Tags

   His8 and Multi1: depending on the LP2 primer, eit…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAV-U6+27-Tornado-RhoBAST-MS2

   Plasmid

   #196342

   Purpose

   Overexpression of circular RhoBAST-MS2

   Depositor

   Murat Sunbul

   Article

   Englert et al Nat Commun. 2023 Jun 30;14(1):3879. doi:

   Insert

   Tornado-RhoBAST-MS2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6+27

   Available since

   Feb. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1/hChR2(H134R)-EYFP

   Plasmid

   #20940

   Purpose

   Mammalian expression of humanized ChR2 with H134R mutation fused to EYFP for optogenetic activation

   Depositor

   Karl Deisseroth

   Article

   Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

   Insert

   channelrhodopsin-2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   H134R mutation in ChR2

   Promoter

   CMV

   Available since

   May 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRU325

    Plasmid

    #167676

    Purpose

    Intermediate vector for eight gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    attL1-ccdB-attL2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCyterm-mScarlet3_N1

    Plasmid

    #189778

    Purpose

    Construct for testing oligimerization of mScarlet3 in mammalian cells by OSER assay

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    Cyterm-mScarlet3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pFS478

    Plasmid

    #89066

    Purpose

    KanMX Z3EV promoter cassette

    Depositor

    Nick Rhind

    Article

    Ohira et al Yeast. 2017 Aug;34(8):323-334. doi: 10.1

    Insert

    Z3EV promoter cassette
    

    Use

    Yeast integration vector

    Tags

    Expression

    Mutation

    yeast Gal1 promoter modified with multiple Zif268…

    Promoter

    Z3EV promoter

    Available since

    July 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. ACPPiso1-V5

    Plasmid

    #113052

    Purpose

    Expresses human prostatic acid phosphatase (isoform 1) in mammalian cells

    Depositor

    Federico Sesti

    Article

    Cotella et al FASEB J. 2013 Apr;27(4):1381-93. doi: 10

    Insert

    prostatic acid phosphatase isoform 1 (ACP3 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. ACPPiso2-V5

    Plasmid

    #113053

    Purpose

    Expresses human prostatic acid phosphatase (isoform 2) in mammalian cells

    Depositor

    Federico Sesti

    Article

    Cotella et al FASEB J. 2013 Apr;27(4):1381-93. doi: 10

    Insert

    prostatic acid phosphatase isoform 2 (ACP3 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDGE1109

    Plasmid

    #202773

    Purpose

    CRISPR-KO with TRE2 exonuclease co-expression in Arabidopsis thaliana

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant Methods. 2023 Mar 28;19(1):30. doi

    Inserts

    FAST marker
    pRPS5a:TREX2-zCas9_ttriple
    ccdB cassette (BsaI-excisable)
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA-NBid-PhoCl2c-CBid

    Plasmid

    #164051

    Purpose

    Optogenetic manipulation of cell apoptosis using PhoCl2c

    Depositor

    Robert Campbell

    Article

    Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

    Insert

    NBid-PhoCl2c-CBid
    

    Use

    Tags

    kozak sequence

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promotor

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV2-CAGIG-LAMP1-mGL

    Plasmid

    #222394

    Purpose

    To express in mammalian cells, the lysosome-associated membrane glycoprotein 1 (LAMP1) fused with the FP protein mGreenLantern. This plasmid used to label lysosomes in transfected and infected cells.

    Depositor

    Pantelis Tsoulfas

    Insert

    LAMP1 and mGreenlantern (LAMP1 Synthetic, Human, Homo Sapiens)
    

    Use

    AAV

    Tags

    LAMP1 and mGreenLantern

    Expression

    Mammalian

    Mutation

    Promoter

    CAG or CAGIG

    Available since

    Sept. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA5/FRT/TO_IgKchL_HA_STf_myc_PDGFRtmb

    Plasmid

    #182009

    Purpose

    CMV overexpression of SNAP-tag fast at the outer leaflet of the plasma membrane of mammalian cell lines

    Depositor

    Kai Johnsson

    Article

    Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

    Insert

    IgKchL_HA_STf_myc_PDGFRtmb
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Fast mutation on SNAP-tag E30R

    Promoter

    CMV

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAC1430

    Plasmid

    #127981

    Purpose

    AMA1 plasmid with Aspergillus optimized Cpf1, pyrG selection marker, and USER cassette (PacI/Nt.BbvCI)

    Depositor

    Uffe Mortensen

    Article

    Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

    Insert

    Cpf1
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Mutation

    codon optimized for Aspergillus nidulans

    Promoter

    Aspergillus nidulans tef1 promoter

    Available since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCfB2312 (TEF1p-Cas9-CYC1t_kanMX)

    Plasmid

    #83946

    Purpose

    Cas9-carrying vector with kanMX marker

    Depositor

    Irina Borodina

    Article

    Stovicek et al Metab Eng Commun. 2015 Mar 20;2:13-22. d

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA5/FRT/TO_NES_STf

    Plasmid

    #182001

    Purpose

    CMV overexpression of SNAP-tag fast strictly in the cytoplasm of mammalian cell lines

    Depositor

    Kai Johnsson

    Article

    Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

    Insert

    NES-STf
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Fast mutation on SNAP-tag E30R

    Promoter

    CMV

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pDGE1108

    Plasmid

    #202772

    Purpose

    CRISPR-KO in Arabidopsis thaliana

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant Methods. 2023 Mar 28;19(1):30. doi

    Inserts

    FAST marker
    pRPS5a:zCas9_ttriple
    ccdB cassette (BsaI-excisable)
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCyterm-mScarlet-I3_N1

    Plasmid

    #189777

    Purpose

    Construct for testing oligimerization of mScarlet-I3 in mammalian cells by OSER assay

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    Cyterm-mScarlet-I3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pEYFP-C3-HA-Ubiquitin

    Plasmid

    #28236

    Depositor

    Michael Mancini

    Article

    Stenoien et al Nat Cell Biol. 2002 Oct . 4(10):806-10.

    Insert

    Ubiquitin (UBB Human)
    

    Use

    Tags

    EYFP and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAAV-Syn-VARNAM

    Plasmid

    #115554

    Purpose

    Red fluorescent voltage indicator for neuronal expression

    Depositor

    Vincent Pieribone

    Article

    Kannan et al Nat Methods. 2018 Dec;15(12):1108-1116.

    Insert

    VARNAM
    

    Use

    AAV

    Tags

    Golgi and ER export signal

    Expression

    Mutation

    Promoter

    human Synapsin

    Available since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAC1749

    Plasmid

    #127983

    Purpose

    AMA1 plasmid with Aspergillus optimized Cpf1, hygB selection marker, and USER cassette (PacI/Nt.BbvCI)

    Depositor

    Uffe Mortensen

    Article

    Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

    Insert

    Cpf1
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Mutation

    codon optimized for Aspergillus nidulans

    Promoter

    Aspergillus nidulans tef1 promoter

    Available since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA5/FRT/TO_Cox8a(4x)-SNAP-tag2-T2A-EGFP

    Plasmid

    #226515

    Purpose

    Mamalian cell expression of SNAP-tag2 in the mitochondrial matrix and cytosolic EGFP

    Depositor

    Kai Johnsson

    Article

    Kühn et al bioRxiv 2024.08.28.610127

    Insert

    Cox8a(4x)-SNAP-tag2-T2A-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pFS462

    Plasmid

    #89065

    Purpose

    his7 integration plasmid with Z3EVpr:GFP

    Depositor

    Nick Rhind

    Article

    Ohira et al Yeast. 2017 Aug;34(8):323-334. doi: 10.1

    Insert

    Z3EV promoter GFP-NLS leu1 C-terminal 300 nt
    

    Use

    S. pombe his7 integration vector

    Tags

    Expression

    Mutation

    Promoter

    Z3EV

    Available since

    July 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pKL2013

    Plasmid

    #172182

    Purpose

    A CRISPR/Cas9 vector targeting maize glossy2 gene for targeted mutagenesis with a mCherry as fluorescent marker.

    Depositor

    Kan Wang

    Article

    McCaw et al Front Genome Ed. 2021 Jan 11;2:622227. d

    Insert

    mCherry
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Cauliflower mosaic virus 35S promoter

    Available since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. MMLV-CAG-G_oT7pol-IRES-BSD

    Plasmid

    #172369

    Purpose

    Generation of oT7pol-expressing stable cell lines, resistant to the antibiotic Blasticidin

    Depositor

    Yoav Ben-Simon, Peter Jonas

    Article

    Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

    Insert

    oT7pol + BSD
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. MMLV-CAG-SADB19_oG-IRES-Puro

    Plasmid

    #172367

    Purpose

    Generation of stable cell lines expressing the rabies optimized glycoprotein (oG), resistant to the antibiotic Puromycin

    Depositor

    Yoav Ben-Simon, Peter Jonas

    Article

    Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

    Insert

    oG + Puro
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pSG5-hSyntenin2a

    Plasmid

    #89432

    Purpose

    mammalian expression of human Syntenin2a

    Depositor

    Fritz Rathjen

    Article

    Koroll et al J Biol Chem. 2001 Apr 6;276(14):10646-54

    Insert

    SDCBP2 (SDCBP2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    May 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pLenti-CaMKIIa-eNpHR-EYFP-WPRE

    Plasmid

    #20946

    Purpose

    3rd gen lentiviral expression of halorhodopsin 2.0 (eNpHR) fused to EYFP driven by CaMKIIa promoter for optogenetic inhibition

    Depositor

    Karl Deisseroth

    Article

    Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

    Insert

    halorhodopsin
    

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIIa

    Available since

    May 29, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. eGFP-CBP

    Plasmid

    #169211

    Purpose

    Expression of eGFP-CBP protein for chitin visualization

    Depositor

    James S Fraser

    Article

    Ricardo-Gonzalez et al bioRxiv 2021.03.29.437438

    Insert

    Chitinase A1 Chitin Binding Domain
    

    Use

    Tags

    6xHis, Mxe Gyr Intein, and eGFP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAV-U6+27-Tornado-Pepper-RhoBAST

    Plasmid

    #196343

    Purpose

    Overexpression of circular Pepper-RhoBAST

    Depositor

    Murat Sunbul

    Article

    Englert et al Nat Commun. 2023 Jun 30;14(1):3879. doi:

    Insert

    Tornado-Pepper-RhoBAST
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6+27

    Available since

    Feb. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. β3-integrin-CFP

    Plasmid

    #26652

    Depositor

    Jonathan Jones

    Article

    Tsuruta et al FASEB J. 2002 Jun . 16(8):866-8.

    Insert

    β3-integrin (ITGB3 Human)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

37. pCoofy48

    Plasmid

    #55186

    Purpose

    Bacterial expression vector for parallel SLIC cloning containing N-terminal 6x His and GST tags. C-terminal tags can be added during SLIC.

    Depositor

    Sabine Suppmann

    Article

    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

    Type

    Empty backbone

    Use

    Tags

    6x His, GST, His10-Stop-OneStrep-Stop-S-tag-Stop-…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pExpreS2-1

    Plasmid

    #175360

    Purpose

    ZeoR backbone for recombinant protein overexpression with Drosophila ExpreS2 Platform

    Depositor

    ExpreS2ion Biotechnologies

    Article

    Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Fused Actin-HSP70

    Available since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAAV-myrSNAP

    Plasmid

    #60098

    Purpose

    Expresses membrane-targeted SNAP(f)-tag and histone-GFP, non-conditional

    Depositor

    Gregory Jefferis

    Article

    Kohl et al Proc Natl Acad Sci U S A. 2014 Aug 25. p

    Inserts

    SNAP-tag
    histone-GFP
    

    Use

    AAV and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. mTubulin NLS-mCherry-LEXY

    Plasmid

    #182597

    Purpose

    Drosophila integration plasmid expressing NLS-mCherry-LEXY fusion protein under the maternal tubulin promoter.

    Depositor

    Jared Toettcher

    Article

    Singh et al Cell Rep. 2022 Mar 22;38(12):110543. doi

    Insert

    mCherry
    

    Use

    Tags

    LEXY and NLS

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge