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Showing: 1901 - 1950 of 2374 results

  1. Spike Display_Part 1 DO

    Plasmid
    #172721
    Purpose
    Encodes a sfGFP dropout expression cassette in place of Part 1 of HexaPro-D614G spike to be used in the Spike Display system
    Depositor
    IIya Finkelstein
    Article
    Javanmardi et al Mol Cell. 2021 Dec 16;81(24):5099-5111.e
    Insert
    SARS-CoV-2 S HexaPro-D614G (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…
    Expression
    Mammalian
    Mutation
    Ectodomain only (AAs 1-1208); 682-685 (furin site…
    Promoter
    CMV
    Available since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    172721-plasmid-map-sequence-id-344440

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  2. pCRISPaint-mNeon-F-gB-PuroR [M1G]

    Plasmid
    #171803
    Purpose
    Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, HSV-1 glycoprotein B CD8+ T cell epitope [AA: 498-505 (SSIEFARL)] and puromycin resistance cassette [p.M1G]
    Depositor
    Michael Hoelzel
    Article
    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.
    Insert
    mNeonGreen-FLAG-gB-T2A-PuroR
    Use
    Gene tagging
    Tags
    Expression
    Mutation
    PuroR: Changed Methionin 1 to Glycine.
    Promoter
    Available since
    Sept. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    171803-plasmid-map-sequence-id-341955

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  3. Spike Display_Part-5 DO

    Plasmid
    #172725
    Purpose
    Encodes a sfGFP dropout expression cassette in place of Part 5 of HexaPro-D614G spike to be used in the Spike Display system
    Depositor
    IIya Finkelstein
    Article
    Javanmardi et al Mol Cell. 2021 Dec 16;81(24):5099-5111.e
    Insert
    SARS-CoV-2 S HexaPro-D614G (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…
    Expression
    Mammalian
    Mutation
    Ectodomain only (AAs 1-1208); 682-685 (furin site…
    Promoter
    CMV
    Available since
    Aug. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    172725-plasmid-map-sequence-id-339289

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  4. Spike Display_Part 3 DO

    Plasmid
    #172723
    Purpose
    Encodes a sfGFP dropout expression cassette in place of Part 3 of HexaPro-D614G spike to be used in the Spike Display system
    Depositor
    IIya Finkelstein
    Article
    Javanmardi et al Mol Cell. 2021 Dec 16;81(24):5099-5111.e
    Insert
    SARS-CoV-2 S HexaPro-D614G (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…
    Expression
    Mammalian
    Mutation
    Ectodomain only (AAs 1-1208); 682-685 (furin site…
    Promoter
    CMV
    Available since
    Aug. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    172723-plasmid-map-sequence-id-339286

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  5. Spike Display_Part-4 DO

    Plasmid
    #172724
    Purpose
    Encodes a sfGFP dropout expression cassette in place of Part 4 of HexaPro-D614G spike to be used in the Spike Display system
    Depositor
    IIya Finkelstein
    Article
    Javanmardi et al Mol Cell. 2021 Dec 16;81(24):5099-5111.e
    Insert
    SARS-CoV-2 S HexaPro-D614G (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…
    Expression
    Mammalian
    Mutation
    Ectodomain only (AAs 1-1208); 682-685 (furin site…
    Promoter
    CMV
    Available since
    Aug. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    172724-plasmid-map-sequence-id-339287

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  6. pCRISPaint-mScarlet-F-gB-PuroR [M1G]

    Plasmid
    #171812
    Purpose
    Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein, FLAG tag, HSV-1 glycoprotein B CD8+ T cell epitope [AA: 498-505 (SSIEFARL)] and puromycin resistance cassette [p.M1
    Depositor
    Michael Hoelzel
    Article
    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.
    Insert
    mScarlet-F-gB-T2A-PuroR
    Use
    Gene tagging
    Tags
    Expression
    Mutation
    PuroR: Changed Methionin 1 to Glycine.
    Promoter
    Available since
    July 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    171812-plasmid-map-sequence-id-337985

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  7. pCRISPaint-mScarlet-F-mgp100-PuroR [M1G]

    Plasmid
    #171811
    Purpose
    Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein, FLAG tag, murine gp100 CD8+ T cell epitope [AA: 25-33 (EGSRNQDWL)] and puromycin resistance cassette [p.M1G]
    Depositor
    Michael Hoelzel
    Article
    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.
    Insert
    mScarlet-F-mgp100-T2A-PuroR
    Use
    Gene tagging
    Tags
    Expression
    Mutation
    PuroR: Changed Methionin 1 to Glycine.
    Promoter
    Available since
    July 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    171811-plasmid-map-sequence-id-337983

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  8. pCRISPaint-mScarlet-F-hgp100-PuroR [M1G]

    Plasmid
    #171810
    Purpose
    Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein, FLAG tag, human gp100 CD8+ T cell epitope [AA: 25-33 (KVPRNQDWL)] and puromycin resistance cassette [p.M1G]
    Depositor
    Michael Hoelzel
    Article
    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.
    Insert
    mScarlet-F-hgp100-T2A-PuroR
    Use
    Gene tagging
    Tags
    Expression
    Mutation
    PuroR: Changed Methionin 1 to Glycine.
    Promoter
    Available since
    July 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    171810-plasmid-map-sequence-id-337982

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  25. pSLIK-NFLAG-hEWSR1G511A-CMyc

    Plasmid
    #50734
    Purpose
    Produces lentivirus expressing human EWSR1G511A mutant with FLAG tag at its N-terminus and Myc tag at its C-terminus by co-transfection with psPAX2 and pMD2G
    Depositor
    Zissimos Mourelatos
    Article
    Couthouis et al Hum Mol Genet. 2012 Jul 1;21(13):2899-91
    Insert
    EWSR1 G511A (EWSR1 Human)
    Use
    Lentiviral
    Tags
    FLAG and Myc
    Expression
    Mutation
    Changed Gly 511 to Ala
    Promoter
    TRE
    Available since
    Jan. 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  26. pSLIK-NFLAG-hEWSR1P552L-CMyc

    Plasmid
    #50736
    Purpose
    Produces lentivirus expressing human EWSR1P552L mutant with FLAG tag at its N-terminus and Myc tag at its C-terminus by co-transfection with psPAX2 and pMD2G
    Depositor
    Zissimos Mourelatos
    Article
    Couthouis et al Hum Mol Genet. 2012 Jul 1;21(13):2899-91
    Insert
    EWSR1 P552L (EWSR1 Human)
    Use
    Lentiviral
    Tags
    FLAG and Myc
    Expression
    Mutation
    Changed Pro 552 to Leu
    Promoter
    TRE
    Available since
    Jan. 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  28. pET32bplus_trx_dAChE4

    Plasmid
    #83917
    Purpose
    Encodes an E. coli codon optimized version of dAChE4 - a designed variant of human acetylcholinesterase bearing 51 mutations with ~1800 fold higher functional expression levels in E. coli
    Depositor
    Sarel Fleishman
    Article
    Goldenzweig et al Mol Cell. 2016 Jul 21;63(2):337-46. doi:
    Insert
    Designed hAChE, codon optimised for E.coli expression (ACHE Human, Synthetic)
    Use
    Tags
    His, S-tag, and TRX
    Expression
    Bacterial
    Mutation
    V13T,K24T,M43V,L49R,V61W,S68N,E92N,T110K,S111N,T1…
    Promoter
    T7 promoter
    Available since
    March 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    83917-plasmid-map-sequence-id-174928

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  29. PB-EEA-5g-OSK2M2L1-PGK-Puro

    Plasmid
    #102909
    Purpose
    PiggyBac transposon system construct for expression of 10 gRNAs targeting OCT4, MYC, KLF4, SOX2 and LIN28A promoters and 5 gRNAs targeting EEA-motif. Includes PGK-puro selection cassette.
    Depositor
    Timo Otonkoski
    Article
    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1
    Insert
    EEA-5g-OSK2M2L1-gRNAs-PGK-Puro (POU5F1, SOX2, KLF4, MYC, LIN28A Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    July 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    102909-plasmid-map-sequence-id-200136

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  30. PB-GG-OMKSL-PGK-Puro

    Plasmid
    #102894
    Purpose
    PiggyBac transposon system construct with 10 concatenated U6 promoter driven transcriptional cassettes targeting OCT4, MYC, KLF4, SOX2 and LIN28A promoters. Contains PGK-puro selection cassette.
    Depositor
    Timo Otonkoski
    Article
    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1
    Insert
    OMKSL-gRNAs-PGK-Puro (POU5F1, MYC, KLF4, SOX2, LIN28A Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    July 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    102894-plasmid-map-sequence-id-198285

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  31. GG-EBNA-OMKSL-PP

    Plasmid
    #102899
    Purpose
    EBNA episome plasmid for U6 promoter-driven expression of 10 gRNAs targeting OCT4, MYC, KLF4, SOX2 and LIN28A promoters. Includes PGK-puro selection cassette.
    Depositor
    Timo Otonkoski
    Article
    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1
    Insert
    OMKSL-gRNAS-PGK-puro (POU5F1, MYC, KLF4, SOX2, LIN28A Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    July 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    102899-plasmid-map-sequence-id-198366

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  32. AiP12787 - pAAV-AiE0140h_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2787)

    Plasmid
    #191726
    Purpose
    AI plasmid ALIAS: AiP12787. Expression of SYFP2 (yellow fluorescent protein) in striatal Pvalb+ neurons. Note strong cortical Pvalb and L5 ET neuron expression
    Depositor
    Jonathan Ting, The Allen Institute for Brain Science
    Article
    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1
    Has Service
    AAV PHP.eB
    Insert
    SYFP2
    Use
    AAV
    Tags
    Expression
    Mutation
    none
    Promoter
    Available since
    Feb. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    191726-plasmid-map-sequence-id-376718

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  35. AiP13808 - pAAV-AiE0140h_3xC2-minBG-mTFP1-WPRE3-BGHpA (Alias: CN3808)

    Plasmid
    #224176
    Purpose
    AiE0140h_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal Pvalb+ neurons (note cortical Pvalb+ and L5_ET)
    Depositor
    Jonathan Ting, The Allen Institute for Brain Science
    Article
    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1
    Insert
    mTFP1
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    224176-plasmid-map-sequence-id-446818

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  36. pOCC102-MBP-eIF4G-Sk

    Plasmid
    #219976
    Purpose
    eIF4G-Sk protein purification
    Depositor
    Simon Alberti
    Insert
    eIF4G-Sk
    Use
    Tags
    6xHis-MBP
    Expression
    Insect
    Mutation
    Promoter
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    219976-plasmid-map-sequence-id-436842

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  37. pOCC102-MBP-eIF4G

    Plasmid
    #219971
    Purpose
    eIF4G protein purification
    Depositor
    Simon Alberti
    Insert
    eIF4G (TIF4631 Budding Yeast)
    Use
    Tags
    6xHis-MBP
    Expression
    Insect
    Mutation
    Promoter
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    219971-plasmid-map-sequence-id-436844

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  38. pOCC102-MBP-Pab1

    Plasmid
    #219979
    Purpose
    Pab1p protein purification
    Depositor
    Simon Alberti
    Insert
    Pab1p (PAB1 Budding Yeast)
    Use
    Tags
    6xHis-MBP
    Expression
    Insect
    Mutation
    Promoter
    Available since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    219979-plasmid-map-sequence-id-436849

    Enlarge

  39. pOCC102-MBP-eIF4A

    Plasmid
    #219981
    Purpose
    eIF4A protein purification
    Depositor
    Simon Alberti
    Insert
    eIF4A (TIF1 Budding Yeast)
    Use
    Tags
    6xHis-MBP
    Expression
    Insect
    Mutation
    Promoter
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    219981-plasmid-map-sequence-id-436878

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  40. pOCC22-MBP-eIF4E

    Plasmid
    #219978
    Purpose
    eIF4E protein purification
    Depositor
    Simon Alberti
    Insert
    eIF4E (CDC33 Budding Yeast)
    Use
    Tags
    MBP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    219978-plasmid-map-sequence-id-436833

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  41. RB793

    Plasmid
    #199121
    Purpose
    This plasmid contains a barcoding cassette which gets integrated at Neut5 locus in Candida albicans genome
    Depositor
    Richard Bennett
    Insert
    100 bp barcoding cassette
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Aug. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    199121-plasmid-map-sequence-id-392171

    Enlarge

  42. RB794

    Plasmid
    #199122
    Purpose
    This plasmid contains a barcoding cassette which gets integrated at Neut5 locus in Candida albicans genome
    Depositor
    Richard Bennett
    Insert
    100 bp barcoding cassette
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Aug. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    199122-plasmid-map-sequence-id-392181

    Enlarge

  43. pUC57-T7-PAB1-NLuc

    Plasmid
    #219984
    Purpose
    PAB1-5'UTR-NLuc mRNA synthesis
    Depositor
    Simon Alberti
    Insert
    PAB1-5'UTR-NLuc (PAB1 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    219984-plasmid-map-sequence-id-436830

    Enlarge

  44. pOCC121-MBP-monoGFP-eIF4G

    Plasmid
    #219972
    Purpose
    eIF4G protein purification
    Depositor
    Simon Alberti
    Insert
    eIF4G (TIF4631 Budding Yeast)
    Use
    Tags
    6xHis and MBP-monoGFP
    Expression
    Insect
    Mutation
    Promoter
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    219972-plasmid-map-sequence-id-436768

    Enlarge

  45. RB818

    Plasmid
    #199146
    Purpose
    This plasmid contains a barcoding cassette which gets integrated at Neut5 locus in Candida albicans genome
    Depositor
    Richard Bennett
    Insert
    100 bp barcoding cassette
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Aug. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    199146-plasmid-map-sequence-id-404730

    Enlarge

  46. RB810

    Plasmid
    #199138
    Purpose
    This plasmid contains a barcoding cassette which gets integrated at Neut5 locus in Candida albicans genome
    Depositor
    Richard Bennett
    Insert
    100 bp barcoding cassette
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Aug. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    199138-plasmid-map-sequence-id-392567

    Enlarge

  47. RB811

    Plasmid
    #199139
    Purpose
    This plasmid contains a barcoding cassette which gets integrated at Neut5 locus in Candida albicans genome
    Depositor
    Richard Bennett
    Insert
    100 bp barcoding cassette
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Aug. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    199139-plasmid-map-sequence-id-392182

    Enlarge

  48. RB812

    Plasmid
    #199140
    Purpose
    This plasmid contains a barcoding cassette which gets integrated at Neut5 locus in Candida albicans genome
    Depositor
    Richard Bennett
    Insert
    100 bp barcoding cassette
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Aug. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    199140-plasmid-map-sequence-id-392175

    Enlarge

  49. RB813

    Plasmid
    #199141
    Purpose
    This plasmid contains a barcoding cassette which gets integrated at Neut5 locus in Candida albicans genome
    Depositor
    Richard Bennett
    Insert
    100 bp barcoding cassette
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Aug. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    199141-plasmid-map-sequence-id-392167

    Enlarge

  50. RB814

    Plasmid
    #199142
    Purpose
    This plasmid contains a barcoding cassette which gets integrated at Neut5 locus in Candida albicans genome
    Depositor
    Richard Bennett
    Insert
    100 bp barcoding cassette
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Aug. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    199142-plasmid-map-sequence-id-392014

    Enlarge

Showing: 1901 - 1950 of 2374 results