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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 2,493 results for: D10

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Showing: 761 - 780 of 2493 results

  2. ACBE

    Plasmid
    #204607
    Purpose
    Mammalian expression of adenine-to-cytosine base editor ACBE
    Depositor
    Dali Li
    Publication
    Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    bpNLS-N terminal of nSpCas9-linker-mAAG (R165E/Y179F)-TadA-8e-linker-C terminal of nSpCas9-bpNLS-P2A-GFP
    Use
    CRISPR
    Tags
    6*His
    Expression
    Mammalian
    Mutation
    mAAG (R165E/Y179F); S. pyogenes Cas9 (D10A)
    Available since
    Aug. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    204607-plasmid-map-sequence-id-404719

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  3. AALN-BE4max

    Plasmid
    #138161
    Purpose
    C-to-T base editor
    Depositor
    David Liu
    Publication
    Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    rAPOBEC1(AALN)-nSpCas9-UGI-UGI
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    Within rAPOBEC1 R33A + K34A + H122L + D124N; with…
    Promoter
    CMV
    Available since
    Feb. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    138161-plasmid-map-sequence-id-272350

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  10. eA3A-BE3 (pRZ206)

    Plasmid
    #131315
    Purpose
    CAG promoter expression plasmid for hA3A(N57G)-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP.
    Depositor
    Keith Joung
    Publication
    Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hA3A(N57G)-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Oct. 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    131315-plasmid-map-sequence-id-254673

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  12. pcDNA3-Flag-Tet1 CD Mut

    Plasmid
    #72218
    Purpose
    Express flag-tagged mouse Tet1 catalytic domain - catalytic dead mutant.
    Depositor
    Yi Zhang
    Publication
    Ito et al Science. 2011 Sep 2;333(6047):1300-3. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Ten-Eleven Translocation 1 (Tet1 Mouse)
    Tags
    flag
    Expression
    Mammalian
    Mutation
    The plasmid expresses TET1 catalytic domain (cata…
    Promoter
    CMV
    Available since
    May 1, 2018
    Availability
    Academic Institutions and Nonprofits only

  13. pCAG-CBE4max-SpRYc-P2A-EGFP

    Plasmid
    #208336
    Purpose
    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRYc
    Depositor
    Pranam Chatterjee
    Publication
    Zhao et al Nat Commun. 2023 Oct 4;14(1):6175. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized CBE4max Cas9 variant named SpRYc with P2A-EGFP
    Use
    CRISPR
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    Sc++ (1-1119), SpRY (1111-1368), D10A
    Promoter
    CAG
    Available since
    Oct. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    208336-plasmid-map-sequence-id-439176

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  14. pME464

    Plasmid
    #238068
    Purpose
    Expressed ME-ABE ABE7.10-HRHSK with SpRYCas9
    Depositor
    Alexis Komor
    Publication
    Evanoff et al Nat Biotechnol. 2026 Mar 18. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TadA*7.10(L36H/L51R/Y123H/C146S/N157K)-m_SpRYnCas9(D10A)
    Use
    CRISPR
    Tags
    SV40 bpNLS and SV40 bpNLS-P2A-mCherry
    Expression
    Mammalian
    Mutation
    TadA modifications include L36H, L51R, Y123H, C14…
    Available since
    Jan. 9, 2026
    Availability
    Academic Institutions and Nonprofits only
    238068-plasmid-map-sequence-id-472664

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  19. dCas9-miniTurbo

    Plasmid
    #219822
    Purpose
    EF1α-driven expression of dCas9 fused to miniTurbo with GFP (Adapted from plasmid #153209)
    Depositor
    Philipp Maass
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9
    Use
    CRISPR
    Tags
    miniTurbo
    Expression
    Mammalian
    Mutation
    D10A/H841A
    Promoter
    EF1a
    Available since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    219822-plasmid-map-sequence-id-482202

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  20. PB-CAG-DDdCas9VP192-T2A-GFP-IRES-Neo

    Plasmid
    #102885
    Purpose
    PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection
    Depositor
    Timo Otonkoski
    Publication
    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DDdCas9VP192-T2A-GFP-IRES-Neo
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    CAG
    Available since
    July 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    102885-plasmid-map-sequence-id-259794

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Showing: 761 - 780 of 2493 results