Addgene

We narrowed to 7,580 results for: Lif;

Showing: 761 - 780 of 7580 results

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pBS-TRE-CLIPf-WPRE

Plasmid

#104107

Purpose

Expresses CLIPf under the TRE promoter in mammalian cells.

Depositor

Takeshi Imai

Article

Sakaguchi et al eLife. 2018 Nov 20;7. pii: 40350. doi: 1

Insert

CLIPf

Use

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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LexAop2-myr::4xSNAPf

Plasmid

#87638

Purpose

For creating drosophila transgenics expressing LexAop2-myr4x::SNAPf

Depositor

Gregory Jefferis

Article

Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

Insert

myr::4xSNAPf

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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pTK645

Plasmid

#114711

Purpose

Express NuMA(1700-2115)-RFP-Nano from Rosa 26 locus

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

NuMA(1700-2115)-RFP-Nano

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pDEW8

Plasmid

#84238

Purpose

Plasmid containing a P2A peptide (porcine teschovirus-1 derived) sequence that works efficiently in Saccharomyces cerevisiae

Depositor

David Weinberg

Article

DiSanto et al Elife. 2016 Oct 1;5. pii: e20069. doi: 1

Insert

HA-mRuby-P2A-eGFP

Use

Tags

HA-mRuby and eGFP

Expression

Yeast

Mutation

Promoter

GPD

Available since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

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UAS-<FRT.STOP<Bxb1

Plasmid

#87643

Purpose

For creating drosophila transgenics expressing UAS-<FRT.STOP<Bxb1

Depositor

Gregory Jefferis

Article

Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

Insert

Bxb1

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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LexAop2-myr::4xCLIPf

Plasmid

#87636

Purpose

For creating drosophila transgenics expressing LexAop2-myr4x::CLIPf

Depositor

Gregory Jefferis

Article

Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

Insert

myr::4xCLIPf

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

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pTK729

Plasmid

#114710

Purpose

Express NuMA(1-705+1700-2115ΔNLS)-RFP-Nano from Rosa 26 locus

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

NuMA(1-705+1700-2115ΔNLS)-RFP-Nano

Use

Tags

Expression

Mutation

Promoter

Available since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

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pU6-crRNA-MS2

Plasmid

#207624

Purpose

Expresses crRNA-MS2 in mammalian cells (with a human-U6 promoter)

Depositor

Jay Shendure

Article

Choi et al Elife. 2025 Feb 12;13:RP98110. doi: 10.7

Insert

crRNA-MS2

Use

CRISPR

Tags

Expression

Mutation

Promoter

hU6

Available since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-LambdaN-MCP

Plasmid

#207626

Purpose

Expresses LambdaN-MCP fusion protein in mammalian cells (with a CMV promoter)

Depositor

Jay Shendure

Article

Choi et al Elife. 2025 Feb 12;13:RP98110. doi: 10.7

Insert

LambdaN-MCP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

pCMV

Available since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-ALFA-MCP

Plasmid

#207628

Purpose

Expresses ALFA-tagged MCP fusion protein in mammalian cells (with a CMV promoter)

Depositor

Jay Shendure

Article

Choi et al Elife. 2025 Feb 12;13:RP98110. doi: 10.7

Insert

ALFA-MCP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

pCMV

Available since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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Tol2-TLCV2

Plasmid

#196331

Purpose

the TLCV2 plasmid with Tol2 recombination sites for integration

Depositor

Itamar Harel

Article

Moses et al eLife. 2023 Oct 24;12:e85960. doi: 10.75

Insert

Cas9-2A-eGFP

Use

CRISPR; Fish expression

Tags

Expression

Bacterial, Mammalian, and Y…

Mutation

Promoter

Tight TRE promoter

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pSX2739

Plasmid

#200765

Purpose

Expresses mKate2-tag fused CDC-42 with 8xMS2(V1) stem loops in C. elegans, and the tissue-specific promoter col-19 is used.

Depositor

Suhong Xu

Article

Hu et al Elife. 2023 Mar 3;12:e82178. doi: 10.755

Insert

8xMS2

Use

Tags

mKate2

Expression

Worm

Mutation

Promoter

col-19

Available since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pDestTol2-QUAS:mApple-he1.1:CFP

Plasmid

#184812

Purpose

Used to generate the Tg(QUAS:mApple; he1.1:CFP)c788 transgenic line

Depositor

Marnie Halpern

Article

Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

Insert

QUAS:mApple-pA; he1.1:CFP

Use

Zebrafish tol2 transgenesis

Tags

Expression

Mutation

Promoter

Available since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-MTS-jAspSnFR3

Plasmid

#203487

Purpose

Mitochondrial directed jAspSnFR3 in lentiviral expression vector

Depositor

Lucas Sullivan

Article

Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

Insert

MTS-jAspSnFR3

Use

Lentiviral

Tags

Mitochondrial localization signal

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pLX304-CAG-jAspSnFR3-mRuby3

Plasmid

#203500

Purpose

jAspSnFR3-mRuby3 in lentiviral expression vector

Depositor

Lucas Sullivan

Article

Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

Insert

jAspSnFR3-mRuby3

Use

Lentiviral

Tags

His-tag and mRuby3

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pBEL2094

Plasmid

#162142

Purpose

Complementation plasmid for MlaE E98A

Depositor

Damian Ekiert, Gira Bhabha

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Use

Tags

Expression

Bacterial

Mutation

MlaF-MlaE(E98A)-(6xHis-MlaD)-MlaC-MlaB

Promoter

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2095

Plasmid

#162143

Purpose

Complementation plasmid for MlaE K205A

Depositor

Damian Ekiert, Gira Bhabha

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Use

Tags

Expression

Bacterial

Mutation

MlaF-MlaE(K205A)-(6xHis-MlaD)-MlaC-MlaB

Promoter

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2097

Plasmid

#162144

Purpose

omplementation plasmid for MlaE D250A

Depositor

Damian Ekiert, Gira Bhabha

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Use

Tags

Expression

Bacterial

Mutation

MlaF-MlaE(D250A)-(6xHis-MlaD)-MlaC-MlaB

Promoter

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2057

Plasmid

#162145

Purpose

Expression plasmid for MlaE Tyr81Bpa mutant for in vivo crosslinking

Depositor

Damian Ekiert, Gira Bhabha

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Use

Tags

Expression

Bacterial

Mutation

MlaF-(6xHis-MlaE, Tyr81Bpa)-MlaD-MlaC-MlaB

Promoter

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2060

Plasmid

#162146

Purpose

Expression plasmid for MlaE Val77Bpa mutant for in vivo crosslinking

Depositor

Damian Ekiert, Gira Bhabha

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Use

Tags

Expression

Bacterial

Mutation

MlaF-(6xHis-MlaE, Val77Bpa)-MlaD-MlaC-MlaB

Promoter

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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