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FRP2350

Plasmid

#127580

Purpose

Tagging plasmid for WTC 846: Encodes the PtetO7.1 promoter and Hyg resistance gene

Depositor

Joerg Stelling

Article

Azizoglu et al Elife. 2021 Aug 3;10. pii: 69549. doi: 1

Insert

PtetO7.1_Flagtag_8Glycine linker

Use

Synthetic Biology; Pcr based tagging

Tags

Flag tag, 8glycine linker

Available Since

Aug. 13, 2019

Availability

Academic Institutions and Nonprofits only

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FRP2371

Plasmid

#127577

Purpose

Repressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, His3MX selection marker

Depositor

Joerg Stelling

Article

Azizoglu et al Elife. 2021 Aug 3;10. pii: 69549. doi: 1

Insert

PRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1

Use

Synthetic Biology; Shuttle vector

Expression

Yeast

Available Since

Aug. 13, 2019

Availability

Academic Institutions and Nonprofits only

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FRP2374

Plasmid

#127579

Purpose

Repressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, Met15 selection marker

Depositor

Joerg Stelling

Article

Azizoglu et al Elife. 2021 Aug 3;10. pii: 69549. doi: 1

Insert

PRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1

Use

Synthetic Biology; Shuttle vector

Expression

Yeast

Available Since

Aug. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pMK33/Cas9

Plasmid

#133781

Purpose

Expresses drosophila-optimized Cas9

Depositor

Norbert Perrimon

Article

Viswanatha et al Elife. 2018 Jul 27;7. pii: 36333. doi: 1

Insert

Drosophila-optimized Cas9

Expression

Insect

Available Since

Jan. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pHR OVA/p2a/mCherry-CaaX

Plasmid

#113030

Purpose

for lentivirus production of ovalbumin and mcherry-caax

Depositor

Ron Vale

Article

Morrissey et al Elife. 2018 Jun 4;7. pii: 36688. doi: 10

Insert

OVA/p2a/mCherry-CaaX

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Available Since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-PFE3-P2A-GFP

Plasmid

#236178

Purpose

AAV version of constitutive PFE3 for ablating excitatory synapses.

Depositor

Don Arnold

Article

Bareghamyan et al eLife. 2025 Apr 29;13:RP103757. doi: 10.

Insert

PFE3-P2A-GFP

Use

AAV

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pDAS12222_U6-pegRNA-BFP

Plasmid

#177181

Purpose

pegRNA cloning plasmid with BFP marker

Depositor

Ervin Welker

Article

Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pCZGY3196

Plasmid

#172696

Purpose

Prgef-1-gfp1-10 Expresses split GFP parts 1-10 in all C. elegans neurons (only shows active fluorescence when binds to GFP11)

Depositor

Yishi Jin

Article

Noma et al Elife. 2017 Aug 2;6. pii: e26376. doi: 1

Insert

Prgef-1

Tags

GFP 1-10

Expression

Worm

Available Since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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rGluA2 (px330:2-guideCas9)

Plasmid

#169439

Purpose

Expression of Cas9 and 2 guides

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

spCas9

Use

CRISPR

Expression

Mammalian

Available Since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-TRE-RandE3-P2A-GFP

Plasmid

#236179

Purpose

AAV version of Tet inducible control plasmid

Depositor

Don Arnold

Article

Bareghamyan et al eLife. 2025 Apr 29;13:RP103757. doi: 10.

Insert

RandE3-P2A-GFP

Use

AAV

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pTK645

Plasmid

#114711

Purpose

Express NuMA(1700-2115)-RFP-Nano from Rosa 26 locus

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

NuMA(1700-2115)-RFP-Nano

Available Since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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GluA2 (px330:2-guideCas9)

Plasmid

#169420

Purpose

Expression of Cas9 and 2 guides

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

spCas9

Use

CRISPR

Expression

Mammalian

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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GluN1 (px330:2-guideCas9)

Plasmid

#169426

Purpose

Expression of Cas9 and 2 guides

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

spCas9

Use

CRISPR

Expression

Mammalian

Available Since

June 21, 2021

Availability

Academic Institutions and Nonprofits only

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rGluA1 (px330:2-guideCas9)

Plasmid

#169437

Purpose

Expression of Cas9 and 2 guides

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

spCas9

Use

CRISPR

Expression

Mammalian

Available Since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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UAS-3xHalo7::CAAX

Plasmid

#87646

Purpose

For creating drosophila transgenics expressing UAS-3xHalo7::CAAX

Depositor

Gregory Jefferis

Article

Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

Insert

3xHalo7::CAAX

Expression

Insect

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-mTurquoise2-WPRE

Plasmid

#104110

Purpose

AAV-mediated expression of mTurquoise2 under the TRE promoter.

Depositor

Takeshi Imai

Article

Sakaguchi et al eLife. 2018 Nov 20;7. pii: 40350. doi: 1

Insert

mTurquoise2

Use

AAV

Promoter

TRE

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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GluA1 (px330:2-guideCas9)

Plasmid

#169417

Purpose

Expression of Cas9 and 2 guides

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

spCas9

Use

CRISPR

Expression

Mammalian

Available Since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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pKanCMV_mRuby3

Plasmid

#172854

Purpose

mRuby3 expressed under the CMV promotor

Depositor

Haining Zhong

Article

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Insert

mRuby3

Expression

Mammalian

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-jAspSnFR3-mRuby3

Plasmid

#203498

Purpose

jAspSnFR3-mRuby3 in lentiviral expression vector

Depositor

Lucas Sullivan

Article

Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

Insert

jAspSnFR3-mRuby3

Use

Lentiviral

Tags

His-tag and mRuby3

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pGEMHE-NLS-mEGFP

Plasmid

#105527

Purpose

T7 promotor drives in vitro mRNA transcription of a mEGFP with a nuclear localisation signal

Depositor

Melina Schuh

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

Nucleoplasmin

Use

In vitro transcription

Tags

mEGFP

Mutation

NLS of nucleoplasmin (encoding amino acids KRPAAT…

Promoter

T7

Available Since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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