Addgene

We narrowed to 1,788 results for: pb

Showing: 761 - 780 of 1788 results

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pBS-f1(+)-GAPDH-IRES-dTomato

Plasmid

#115912

Purpose

donor to insert IRES-dTomato at the GAPDH locus in human cells

Depositor

Erik Sontheimer

Article

Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

Insert

IRES-dTomato

Use

Donor template

Tags

dTomato

Expression

Mutation

Promoter

Available since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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pBS-f1(+)-ACTB-iTagRFP-T

Plasmid

#115915

Purpose

donor to insert iTagRFP-T at the ACTB locus in human cells

Depositor

Erik Sontheimer

Article

Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

Insert

ACTB-TagRFP-T (ACTB Human)

Use

Donor template

Tags

TagRFP-T

Expression

Mutation

Promoter

Available since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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PB-PGK-Cit-tvhc-DHFR

Plasmid

#116048

Purpose

Reporter plasmid for measuring protease activity

Depositor

Michael Elowitz

Article

Gao et al Science. 2018 Sep 21;361(6408):1252-1258

Insert

Cit-tvhc-DHFR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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PB-CMV-TO-Casp3te-tv-cx

Plasmid

#116056

Purpose

Reporter plasmid for measuring protease activity

Depositor

Michael Elowitz

Article

Gao et al Science. 2018 Sep 21;361(6408):1252-1258

Insert

Casp3te-tv-cx

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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PB-CMV-TO-Casp-rastvTEV-teTVMV2

Plasmid

#116098

Purpose

Expresses a combination of proteases and their reporter

Depositor

Michael Elowitz

Article

Gao et al Science. 2018 Sep 21;361(6408):1252-1258

Insert

Casp-rastvTEV-teTVMV2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 22, 2018

Availability

Academic Institutions and Nonprofits only

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PB-PGK-Cit-tehc-DHFR

Plasmid

#116046

Purpose

Reporter plasmid for measuring protease activity

Depositor

Michael Elowitz

Article

Gao et al Science. 2018 Sep 21;361(6408):1252-1258

Insert

Cit-tehc-DHFR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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PB-CMV-Cit-tehc-DHFR

Plasmid

#116058

Purpose

Reporter plasmid for measuring protease activity

Depositor

Michael Elowitz

Article

Gao et al Science. 2018 Sep 21;361(6408):1252-1258

Insert

Cit-tehc-DHFR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pBS-Histone H3 imperfect loop

Plasmid

#45087

Depositor

Phillip Newmark

Article

Rouhana et al Development. 2014 Jul;141(13):2592-601.

Insert

Histone H3 imperfect stem

Use

RNAi; In vitro transcription

Tags

Expression

Mutation

stem loop sequence is naturally occurring mutant …

Promoter

T7 sense, T3 antisense

Available since

May 30, 2013

Availability

Academic Institutions and Nonprofits only
pBS Matrilin-4 (clone 1_35)

Plasmid

#19192

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

Matrilin 4 isoform 1 precursor (MATN4 Chicken)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
pBS Matrilin-4 (clone 2_49)

Plasmid

#19218

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

Matrilin 4 isoform 1 precursor (MATN4 Chicken)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
pBS Su(fu) (CT#117)

Plasmid

#13858

Depositor

Cliff Tabin

Article

Pearse et al Dev Biol. 1999 Aug 15. 212(2):323-36.

Insert

Su(fu) (SUFU Chicken)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 9, 2007

Availability

Academic Institutions and Nonprofits only
pBS hTSP2 clone M1-B

Plasmid

#12412

Depositor

Paul Bornstein

Insert

M1-B TSP2 (THBS2 Human)

Use

Tags

Expression

Bacterial

Mutation

Fragment from Heparin Binding domain and Type 1 r…

Promoter

Available since

Oct. 23, 2006

Availability

Academic Institutions and Nonprofits only
XZ091 (PB) CMV-sCCL20 (PuroR-BFP)

Plasmid

#233434

Purpose

PiggyBac donor plasmid, secreted CCL20

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

CCL20 (CCL20 Human)

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pBS-EF1afull-H2B-mCherry-P2A-Fluc

Plasmid

#207253

Purpose

Lentiviral transfer genome encoding H2B-mCherry-P2A-Fluc driven by a full-length EF1a promoter

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

H2B-mCherry-P2A-Fluc

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1alpha_full

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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iF58 PB-Zim3-dCas9-mScarlet-Puro

Plasmid

#204728

Purpose

Constitutive CRISPRi by Zim3-dCas9

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Zim3-dCas9

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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iC10 PB-zim3-mycNLS-mApple-hygro

Plasmid

#204719

Purpose

Constitutive CRISPRi by Zim3-dCas9

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Zim3-dCas9

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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PB-CT3G-CERP2-MG-IRES2-mNeonGreen

Plasmid

#175508

Purpose

All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mNeonGreen (CMVe-hEF1a-driven rtTA and puromycin resistance)

Depositor

George Church

Article

Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

TRE3G-mCMV

Available since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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PB-TAC-ERP2-(N-HA)KRAS_G12D

Plasmid

#201254

Purpose

Dox-inducible overexpression of HA-tagged KRASG12D and mCherry in human cells

Depositor

Alexander Kleger

Article

Breunig et al Cell Stem Cell. 2021 Jun 3;28(6):1105-11

Insert

KRAS (KRAS Human)

Use

Piggybac transposon

Tags

HA-Tag

Expression

Mammalian

Mutation

changed Gly (G) 12 to Asp (D)

Promoter

TRE

Available since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

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iF53 PB-Zim3-dCas9-mScarlet-Hygro

Plasmid

#204726

Purpose

Constitutive CRISPRi by Zim3-dCas9

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Zim3-dCas9

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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PB-PG13-mCherry-EF1a-PuroR-EGFP

Plasmid

#195292

Purpose

piggyBac transposon vector with mCherry reporter containing 13 copies of the p53-binding consensus sequence (constitutive puromycin resistance and EGFP)

Depositor

Hideyuki Oguro

Article

Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

Inserts

mCherry
PuroR-P2A-EGFP

Use

Piggybac transposon

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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