-
Plasmid#183629PurposepHR-R(ARE-minCMV-BFP-Triplex-2X crGFP*ABE3-SV40pA)-EFS-Hygro-T2A-rtTA-WPREDepositorInsertCas12 Guide (crGFP*)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLQ11443
Plasmid#183627PurposepHR-R(NFAT-minCMV-BFP-Triplex-2X crGFP*ABE3-SV40pA)-EFS-Hygro-T2A-rtTA-WPREDepositorInsertCas12 Guide (crGFP*)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLQ11434
Plasmid#183625PurposepHR-R(HRE-minCMV-BFP-Triplex-2X crGFP*ABE3-SV40pA)-EFS-Hygro-T2A-rtTA-WPREDepositorInsertCas12 Guide (crGFP*)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLQ11435
Plasmid#183626PurposepHR-R(CRE-minCMV-BFP-Triplex-2X crGFP*ABE3-SV40pA)-EFS-Hygro-T2A-rtTA-WPREDepositorInsertCas12 Guide (crGFP*)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFBCPDL6
Plasmid#67285PurposeCPD photolyase from Drosophila with Flag tag in pFastbac vector. Intermediate in insect cell expressionDepositorInsertCPD photolyase (phr Fly)
UseBacterial vector, insert not expressedTagsFlagExpressionMutationV275E*PromoterAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
aTY171_pPGK1_iRFP_WPRE
Plasmid#225351PurposeLentiviral vector for iRFP expressionDepositorInsertiRFP670
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
aTY173_pPGK1_mTagBFP2_WPRE
Plasmid#225352PurposeLentiviral vector for BFP expressionDepositorInsertBFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClCry4 Y319D
Plasmid#160649PurposeExpression of protein for various experiments including finding interaction partnersDepositorInsertClcry4 PHR Y319D
UseTagsHexa hisExpressionBacterialMutationPromoterAvailable sinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
MYR-FR-C2D-EGFR
Plasmid#179262PurposeLight-induced clustering of EGFR cytosolic domainsDepositorInsertEGFR (EGFR Human)
UseLentiviralTagsCry2 PHR, FUS, FusionRed, and Myristoylation sequ…ExpressionMutationPromoterSFFVAvailable sinceFeb. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCIBN-hTRF2-tagRFP-T
Plasmid#103812PurposeBLInCR 'Localizer' construct that marks telomeres and is targeted by a PHR-tagged effector upon illumination with blue light; can be visualized without triggering PHR recruitmentDepositorUseTagsExpressionMammalianMutationhTRF2: deletion of amino acids 1-42 and 476 compa…PromoterCMVAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCIBN-TetR-tagRFP-T
Plasmid#103809Purpose'Localizer' construct that marks tetO arrays and is targeted by a PHR-tagged effector upon illumination with blue light; can be visualized without triggering PHR recruitmentDepositorUseTagsExpressionMammalianMutationTetR: A4G (M2V)PromoterCMVAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
PKMC356_pTRE-ActivinA_IRES_BFP_WPRE
Plasmid#225355PurposeLentiviral vector for ActivinA and BFP expressionDepositorInsertActvin A and TagBFP (Inhba Mouse, Synthetic)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-OptoFGFR1
Plasmid#59776PurposeExpresses myristoylation signal peptide-FGFR1 cytoplasmic region-PHR domain of cryptochrome2-mCitrine fused proteinDepositorInsertOptoFGFR1 (FGFR1 Human, Mustard Weed)
UseTagsmCitrineExpressionMammalianMutationPromoterCMVAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCIBN-hTRF1-tagRFP-T
Plasmid#103811PurposeBLInCR 'Localizer' construct that marks telomeres and is targeted by a PHR-tagged effector upon illumination with blue light; can be visualized without triggering PHR recruitmentDepositorUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-OptoFGFR1-D387A
Plasmid#59778PurposeExpresses optoFGFR1 with mutation in PHR to suppress homointeraction, thus cannot be activated by lightDepositorInsertOptoFGFR1-D387A (FGFR1 Human, Mustard Weed)
UseTagsmCitrineExpressionMammalianMutationPromoterCMVAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCIBN-LacI-tagRFP-T
Plasmid#103810Purpose'Localizer' construct that marks lacO arrays and is targeted by a PHR-tagged effector upon illumination with blue light; can be visualized without triggering PHR recruitmentDepositorUseTagsExpressionMammalianMutationLacI: C19T (silent, L7L), T264C (silent, A88A), C…PromoterCMVAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
OVC Extended Collection
Plasmid Kit#1000000165PurposeFour plasmids containing the cloning cassette, eight plasmids containing LSDs engineered to heterodimerize and their binding partners, and five plasmids with different light-activated Opto-caspase 9.DepositorAvailable sinceOct. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMH0004
Plasmid#174087PurposeUCOE-SFFV-Cas9-BFPDepositorInsertCas9-BFP
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceAug. 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMH0007
Plasmid#174162PurposeUCOE-EF1A-Cas9-BFPDepositorInsertCas9-BFP
UseLentiviralTagsExpressionMutationPromoterEF1AAvailable sinceAug. 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSLQ8079 - IL2RG GFP-P2A CLIP Donor
Plasmid#202690PurposeCLIP donor template for integrating GFP into IL2RG locus.DepositorInsertCopGFP
UseTagsExpressionMutationPromoterAvailable sinceJuly 20, 2023AvailabilityAcademic Institutions and Nonprofits only