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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,217 results for: sgRNA

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Showing: 761 - 780 of 9217 results

  1. pHSG1C3

    Plasmid
    #164423
    Purpose
    single guide RNA (sgRNA) and Prime editing guide RNA (pegRNA) cloning and mammalian cell expression. BbsI cloning for sgRNAs and BbsI/PstI for pegRNAs.
    Depositor
    Xiao Wang
    Publication
    Standage-Beier et al ACS Synth Biol. 2021 Feb 19;10(2):422-42
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Aug. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    164423-plasmid-map-sequence-id-338872

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  20. pX330_ATP1A1_G7_Dual_sgRNA

    Plasmid
    #173206
    Purpose
    Coselection for HDR in human cells. Vector for dual expression of ATP1A1 G7 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.
    Depositor
    Yannick Doyon
    Publication
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ATP1A1 G7 sgRNA + user-specified sgRNA + SpCas9
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    Dual U6 promoters
    Available since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    173206-plasmid-map-sequence-id-339514

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Showing: 761 - 780 of 9217 results