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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 6,588 results for: siae

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Showing: 761 - 780 of 6588 results

  1. pEVA1

    Plasmid
    #58953
    Purpose
    Contains a synthetic DNA sequence, VAR1u, that specifies the Var1 protein. The Cox4 targeting signal is fused to the N-terminus to direct import of Var1. Complements var1 mutations in mtDNA.
    Depositor
    Thomas Fox, Thomas Mason
    Article
    Sanchirico et al EMBO J. 1998 Oct 1;17(19):5796-804.
    Insert
    VAR1u, VAR1 recoded for expression from the nucleus
    Use
    Yeast ecoli shuttle vector
    Tags
    preCox4 mitochondrial matrix targeting signal
    Expression
    Yeast
    Mutation
    Synthetic gene in standard genetic code
    Promoter
    ADH1
    Available Since
    Aug. 15, 2014
    Availability
    Academic Institutions and Nonprofits only

  2. pCRISPEY-GAL-Kan-ADE2

    Plasmid
    #232105
    Purpose
    Expresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.
    Depositor
    Jeffrey Lewis
    Article
    Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:
    Insert
    ADE2 gRNA and repair template
    Use
    CRISPR
    Expression
    Yeast
    Mutation
    Repair template introduces C at nt 466 of ADE2 to…
    Promoter
    GAL7
    Available Since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    232105-plasmid-map-sequence-id-462309

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  5. pYSD093

    Plasmid
    #252983
    Purpose
    Encodes MYC-tagged S. cerevisiae Aga1 del N181aa disp anchor as a Type 4a part to be used in the yeast secretion/display (YSD2.0) extension of the MoClo yeast toolkit (YTK)
    Depositor
    Paul Young
    Article
    Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102
    Insert
    MYC-tagged S. cerevisiae Aga1 del N181aa display anchor
    Expression
    Bacterial
    Available Since
    April 22, 2026
    Availability
    Academic Institutions and Nonprofits only
    252983-plasmid-map-sequence-id-507347

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  7. pCfB8709

    Plasmid
    #175224
    Purpose
    Xenopus oocyte expression vector containing YOL092W. Contains T7 RNA polymerase binding site, and adds polyA tail to YOL092W. Used for generation of mRNA, for injection into xenopus laevis oocytes.
    Depositor
    Irina Borodina
    Article
    Moller-Hansen et al Front Microbiol. 2024 Apr 12;15:1376653.
    Insert
    YOL092W (CEN.PK strain)
    Use
    Mrna expression, injection into xenopus oocytes
    Tags
    PolyA
    Promoter
    T7
    Available Since
    Aug. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    175224-plasmid-map-sequence-id-347995

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  12. pML107

    Plasmid
    #67639
    Purpose
    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains LEU2 marker for yeast transformation
    Depositor
    John Wyrick
    Article
    Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1
    Inserts
    Cas9
    single guide RNA expression cassette
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    pSNR52 and pTDH3 (aka GAP promoter)
    Available Since
    Aug. 31, 2015
    Availability
    Academic Institutions and Nonprofits only
    67639-plasmid-map-sequence-id-220324

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  13. pCSN067

    Plasmid
    #101748
    Purpose
    Single copy yeast plasmid expressing Cpf1 from Lachnospiraceae bacterium ND2006 (LbCpf1), codon optimized for expression in Saccharomyces cerevisiae.
    Depositor
    Rene Verwaal
    Article
    Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278
    Inserts
    Cpf1 from Lachnospiraceae bacterium ND2006 (LbCpf1) codon optimized for expression in S. cerevisiae.
    KanMX marker expression cassette.
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Yeast
    Promoter
    Heterologous TEF1 promoter from A. gossypii. and …
    Available Since
    Dec. 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    101748-plasmid-map-sequence-id-199897

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  20. pRS415-STE3pr-MFA-Igkappa-FAPoptim-STE3-K424R

    Plasmid
    #221119
    Purpose
    FAP tagged STE3-K424R mutant (N-terminally tagged, optimized) under the Ste3 promoter with 2xMYC tag and MFA1 signal sequence to help target construct to the ER
    Depositor
    Allyson O’Donnell
    Article
    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi
    Insert
    MFA-IgKappa-FAPoptim-STE3-K424R
    Expression
    Yeast
    Mutation
    STE3 mutantK424R
    Promoter
    STE3
    Available Since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    221119-plasmid-map-sequence-id-502434

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Showing: 761 - 780 of 6588 results