We narrowed to 41,072 results for: unk

1. GFP-BRD4

   Plasmid

   #65378

   Purpose

   GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

   Depositor

   Kyle Miller

   Article

   Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

   Insert

   BRD4 (BRD4 Human)
   

   Use

   Tags

   EmGFP

   Expression

   Mammalian

   Mutation

   R1232W

   Promoter

   CMV

   Available Since

   June 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. TIGRE docking site targeting vector

   Plasmid

   #61580

   Purpose

   Create a docking site in the mouse TIGRE genomic locus that allows modification in ES cells by Flp recombinase-mediated cassette exchange

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

   Type

   Empty backbone

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHR-BRD4ΔN-mCherry-sspB

   Plasmid

   #121968

   Purpose

   Expresses fusion of disordered protein BRD4(462-1362), fluorescent protein mCherry, and sspB which upon light activation binds to iLID.

   Depositor

   Clifford Brangwynne

   Article

   Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

   Insert

   BRD4 (BRD4 Human)
   

   Use

   Lentiviral

   Tags

   mCherry-sspB

   Expression

   Mammalian

   Mutation

   Deleted amino acids 1-461

   Promoter

   SFFV

   Available Since

   April 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pHAGE-mt-mKeima-P2A-FRB-Fis1

   Plasmid

   #135295

   Purpose

   stably express both mKeima and FRB-Fis1 for CID induced mitophagy in mammalian cells

   Depositor

   Richard Youle

   Article

   Vargas et al Mol Cell. 2019 Apr 18;74(2):347-362.e6.

   Insert

   mt-mKeima-P2A-FRB-Fis1 (FIS1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   mt-mKeima is fused with FRB-Fis1 via P2A seqeunce

   Promoter

   Available Since

   Jan. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. AAV pmSyn1-EBFP-Cre

   Plasmid

   #51507

   Purpose

   Can be used to generate AAV virus that will express Cre in neurons from the synapsin promoter

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

   Has Service

   AAV5

   Insert

   EBFP-Cre
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   pmSyn1

   Available Since

   July 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Ai65(RCFL-tdT) targeting vector

   Plasmid

   #61577

   Purpose

   Target a Flp and Cre-dependent tdTomato expression cassette to the mouse Rosa26 locus

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

   Insert

   pCAG-FSF-LSL-tdTomato
   

   Use

   Cre/Lox and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available Since

   June 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. GFP-Bcl2-Cb5

   Plasmid

   #18000

   Depositor

   Clark Distelhorst

   Article

   Wang et al J Biol Chem. 2001 Nov 23. 276(47):44117-

   Insert

   ER targeted Bcl-2 (BCL2 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Bcl-2's C terminal targeting sequence replac…

   Promoter

   CMV

   Available Since

   June 4, 2008

   Availability

   Academic Institutions and Nonprofits only

8. TAPBPL pmRFP-N2 (721)

   Plasmid

   #62000

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

   Insert

   TAPBPL (TAPBPL Human)
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

9. GFP-Bcl2-Maob

   Plasmid

   #18001

   Depositor

   Clark Distelhorst

   Article

   Wang et al J Biol Chem. 2001 Nov 23. 276(47):44117-

   Insert

   Mitochondria-targeted Bcl-2 (BCL2 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Bcl-2's C terminal replaced with mitochondri…

   Promoter

   CMV

   Available Since

   June 4, 2008

   Availability

   Academic Institutions and Nonprofits only

10. AAV pTRE-FLEX-EGFP-WPRE-bGHpA

    Plasmid

    #65449

    Purpose

    Can be used to generate AAV virus that will express EGFP from the tetO promoter in a Cre-dependent manner

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

    Insert

    EGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    tetO

    Available Since

    July 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJFA344C7

    Plasmid

    #49236

    Purpose

    TET1-catalytic domain (CD) expression vector for TALE-TET1 fusion protein to induce targeted demethylation in mammalian cells

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    TALE-TET1CD Backbone (TET1 Human, Synthetic)
    

    Use

    TALEN; Tale-tet1 expression vector

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Dec. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pACT1:Cas9-GFP, U6:sgTK

    Plasmid

    #122852

    Purpose

    Expresses Cas9 fused with GFP and a sgRNA targeting the Cryptosporidium parvum TK gene

    Depositor

    David Sibley

    Article

    Wilke et al Cell Host Microbe. 2019 Jun 18. pii: S19

    Inserts

    Cas9-GFP
    U6-sgTK
    

    Use

    CRISPR; Cas9-gfp plasmid for genome editing of cr…

    Tags

    eGFP

    Expression

    Mutation

    Promoter

    Cryptosporidium parvum U6 and Cryptosporidium par…

    Available Since

    July 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pACT1:Cas9-GFP, U6:sgUPRT

    Plasmid

    #122853

    Purpose

    Expresses Cas9 fused with GFP and a sgRNA targeting the Cryptosporidium parvum UPRT gene

    Depositor

    David Sibley

    Article

    Wilke et al Cell Host Microbe. 2019 Jun 18. pii: S19

    Inserts

    Cas9-GFP
    U6-sgUPRT
    

    Use

    CRISPR; Cas9-gfp plasmid for genome editing of cr…

    Tags

    eGFP

    Expression

    Mutation

    Promoter

    Cryptosporidium parvum U6 and Cryptosporidium par…

    Available Since

    July 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHR-BRD4ΔN-miRFP670-Cry2WT

    Plasmid

    #122439

    Purpose

    Expresses disordered protein BRD4(462-1362) fused with fluorescent protein miRFP670 and optogenetic protein Cry2WT

    Depositor

    Clifford Brangwynne

    Article

    Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

    Insert

    BRD4 (BRD4 Human)
    

    Use

    Lentiviral

    Tags

    Cry2WT and miRFP670

    Expression

    Mammalian

    Mutation

    Deleted amino acids 1-461

    Promoter

    SFFV

    Available Since

    April 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJFA334E9

    Plasmid

    #49237

    Purpose

    TET1-catalytic domain (CD) expression vector for TALE-TET1 fusion protein to induce targeted demethylation in mammalian cells

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    TET1 tet methylcytosine dioxygenase 1 (TET1 Human, Synthetic)
    

    Use

    TALEN; Tale-tet1 expression vector

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Dec. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Flag-Bcl2-Cb5

    Plasmid

    #18004

    Depositor

    Clark Distelhorst

    Article

    Wang et al J Biol Chem. 2001 Nov 23. 276(47):44117-

    Insert

    ER targeted Bcl-2 (BCL2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    C terminal of Bcl-2 replaced with Cytochrome b5. …

    Promoter

    CMV

    Available Since

    June 4, 2008

    Availability

    Academic Institutions and Nonprofits only

17. Flag-Bcl2-Maob

    Plasmid

    #18005

    Depositor

    Clark Distelhorst

    Article

    Wang et al J Biol Chem. 2001 Nov 23. 276(47):44117-

    Insert

    Mitochondria-targeted Bcl-2 (BCL2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    C terminal of Bcl-2 replaced with Maob. Targets B…

    Promoter

    CMV

    Available Since

    June 4, 2008

    Availability

    Academic Institutions and Nonprofits only

18. pJFA274

    Plasmid

    #49239

    Purpose

    TET1-catalytic domain (CD) expression vector for TALE-TET1 fusion protein to induce targeted demethylation in mammalian cells

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    TET1 tet methylcytosine dioxygenase 1 (TET1 Human, Synthetic)
    

    Use

    TALEN; Tale-tet1 expression vector

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Dec. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Ai95(RCL-GCaMP6f) targeting vector

    Plasmid

    #61579

    Purpose

    Target a Cre-dependent GCaMP6-fast expression cassette to the mouse Rosa26 locus

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

    Insert

    CAG-LSL-GCaMP6-fast
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available Since

    July 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. GIV-FLAG S245A (SA)

    Plasmid

    #86095

    Purpose

    Expression GIV in mammalian cell

    Depositor

    Pradipta Ghosh

    Article

    Aznar et al Elife. 2016 Nov 4;5. pii: e20795. doi: 1

    Insert

    GIV/Girdin (CCDC88A Human)
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    S245A

    Promoter

    CMV

    Available Since

    Feb. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge