167,795 results

1. pZR071_SFFV-dCas9-mCherry-KRAB

   Plasmid

   #180264

   Purpose

   Lentiviral expression vector for dCas9-KRAB in mammalian cells

   Depositor

   Alexander Marson

   Article

   Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   KRAB and mCherry

   Expression

   Mammalian

   Mutation

   Nuclease dead Cas9

   Promoter

   SFFV

   Available Since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSGKP-spe

   Plasmid

   #117234

   Purpose

   A sgRNA expression plasmid for genome editing in Klebsiella Pneumoniae

   Depositor

   Quanjiang Ji

   Article

   Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Available Since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKT2/CAGXSP/CD63mScarlet

   Plasmid

   #182972

   Purpose

   Stably express CD63 fused with mScarlet in mammalian cells via the sleeping beauty transposon system.

   Depositor

   Masamitsu Kanada

   Article

   Perez et al Adv Genet (Hoboken). 2022 Jan 19;3(1):21

   Insert

   CD63-mScarlet (CD63 Human)
   

   Expression

   Mammalian

   Mutation

   N/A

   Available Since

   April 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. eMscL WT

   Plasmid

   #107454

   Purpose

   eMscL WT is optimized for mammalian rodent neuronal expression to the plasma membrane through a neuron-specific promoter and a voltage-gated channel targeting motif

   Depositor

   Francesco Difato

   Article

   Soloperto et al J Cell Sci. 2018 Jan 29. pii: jcs.210393

   Insert

   bacterial mechanosensitive ion channel of large conductance (mscL Escherichia Coli)
   

   Use

   Adeno-associated viral

   Tags

   Kir2.1 ER export signal (FCYENEV) and tdTomato fl…

   Expression

   Mammalian

   Promoter

   human synapsin 1

   Available Since

   April 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. HA GSK3 beta K85A pcDNA3

   Plasmid

   #14755

   Depositor

   Jim Woodgett

   Article

   Hughes et al EMBO J. 1993 Feb . 12(2):803-8.

   Insert

   GSK3 beta K85A (GSK3B Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K85A. Kinase dead mutant.

   Available Since

   April 12, 2007

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. qTAG-AAVS1-PGK-Zeo

   Plasmid

   #227271

   Purpose

   Empty AAVS1 targeting donor for the insertion of Zeo and a medium strength PGK promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHFS:SEP-rGluA2 TKIT donor

   Plasmid

   #169440

   Purpose

   Donor DNA for TKIT knockin of SEP to GluA2 in rat

   Depositor

   Richard Huganir

   Article

   Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

   Insert

   Super ecliptic pHluorin (SEP)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV_hU6-sgRNA_hUbC-dCas9-ZIM3-KRAB-T2a-PuroR

   Plasmid

   #172982

   Purpose

   control & cloning vector for CRISPRi expressing dead Cas9-ZIM3-KRAB fusion

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   control sgRNA
   

   Use

   CRISPR and Lentiviral

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBAD-Lime

   Plasmid

   #191255

   Purpose

   Bacterial expression plasmids for pH sensitive fluorescent protein Lime

   Depositor

   Robert Campbell

   Article

   Shen et al ACS Sens. 2023 Aug 25;8(8):3014-3022. do

   Insert

   Lime
   

   Tags

   His tag

   Expression

   Bacterial

   Available Since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEX-4T1-RhoA G17A

    Plasmid

    #69357

    Purpose

    expression of nucleotide free RhoA in bacterial cells

    Depositor

    Rafael Garcia-Mata

    Article

    Garcia-Mata et al Methods Enzymol. 2006;406:425-37.

    Insert

    RhoA (RHOA Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    G17A

    Promoter

    tac

    Available Since

    Sept. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pAAV-syn-flex-iGluSnFR4s-NGR-WPRE (AAV1)

    Viral Prep

    #234441-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV-syn-flex-iGluSnFR4s-NGR-WPRE (#234441). In addition to the viral particles, you will also receive purified pAAV-syn-flex-iGluSnFR4s-NGR-WPRE plasmid DNA. Syn-driven, iGluSnFR4s-NGR sensor expression. These AAV preparations are suitable purity for injection into animals.

    Depositor

    GENIE Project

    Article

    Aggarwal et al bioRxiv 2025.03.20.643984

    Promoter

    Syn

    Available Since

    Sept. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

12. pLG009 (DRT type 1)

    Plasmid

    #157887

    Purpose

    Expresses the DRT type 1 defense system

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2020 Aug 28;369(6507):1077-1084

    Insert

    DRT type 1 (RT-nitrilase)
    

    Expression

    Bacterial

    Available Since

    Nov. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX6p1-HH1713

    Plasmid

    #124994

    Purpose

    E. coli expression of recombinant H. hepaticus antigen HH_1713

    Depositor

    Dan Littman

    Article

    Xu et al Nature. 2018 Feb 15;554(7692):373-377. d

    Insert

    HH_1713
    

    Expression

    Bacterial

    Available Since

    July 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGP-AAV-syn-FLEX-jGCaMP7s-WPRE (AAV1)

    Viral Prep

    #104491-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pGP-AAV-syn-FLEX-jGCaMP7s-WPRE (#104491). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP7s-WPRE plasmid DNA. Synapsin-driven, Cre-dependent, GCaMP7s calcium sensor. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Douglas Kim, GENIE Project

    Article

    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

    Promoter

    Syn

    Available Since

    Aug. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

15. pST39-pNL29

    Plasmid

    #91696

    Purpose

    Expresses Mega GVs in E.Coli

    Depositor

    Mikhail Shapiro

    Article

    Lakshmanan et al Nat Protoc. 2017 Oct;12(10):2050-2080. d

    Insert

    Mega GV gene cluster (from pNL29) in pST39 backbone
    

    Tags

    No tags

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRPF185

    Plasmid

    #106367

    Purpose

    C. difficile inducible expression system. Shuttle plasmid containing a tetracycline-inducible gusA

    Depositor

    Robert Fagan, Neil Fairweather

    Article

    Fagan et al J Biol Chem. 2011 Aug 5;286(31):27483-93

    Insert

    Term(fdx)-Ptet-gusA-Term(slpA) (gusA )
    

    Use

    E. coli - c. difficile shuttle vector

    Mutation

    codon optimised for C. difficile

    Promoter

    Ptet

    Available Since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGP-AAV-syn-jGCaMP7s-WPRE (AAV Retrograde)

    Viral Prep

    #104487-AAVrg

    Purpose

    Ready-to-use AAV Retrograde particles produced from pGP-AAV-syn-jGCaMP7s-WPRE (#104487). In addition to the viral particles, you will also receive purified pGP-AAV-syn-jGCaMP7s-WPRE plasmid DNA. Synapsin-driven GCaMP7s calcium sensor. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Douglas Kim, GENIE Project

    Article

    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

    Promoter

    Syn

    Available Since

    May 31, 2018

    Availability

    Academic Institutions and Nonprofits only

18. pAVU6+27-F30-2xdBroccoli

    Plasmid

    #66842

    Purpose

    Expresses two dimeric Broccoli aptamers in the F30 scaffold in mammalian cells

    Depositor

    Samie Jaffrey

    Article

    Filonov et al Chem Biol. 2015 May 21;22(5):649-60. doi

    Insert

    Two dimeric Broccoli aptamers in the F30 scaffold
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pmGFP-ADAR2

    Plasmid

    #117929

    Purpose

    ADAR2 expression plasmid.

    Depositor

    Kumiko Ui-Tei

    Article

    Galipon et al Genes (Basel). 2017 Feb 11;8(2). pii: ge

    Insert

    ADAR2 (ADARB1 Human)
    

    Tags

    myc-GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. 2X_pX458_pSpCas9(BB)-2A-GFP

    Plasmid

    #172221

    Purpose

    Cas9-2A-GFP expression vector bearing two (one additional) independent sgRNA cassettes.

    Depositor

    Alexander Meissner

    Article

    Wu et al Nat Commun. 2021 Aug 12;12(1):4897. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Promoter

    Cbh

    Available Since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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