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Plasmid#180503PurposeHuman CD63-L7Ae fusion proteinDepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHuman CD63-L7Ae fusion protein
ExpressionMammalianPromoterCMVAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
DOX_SAM_CRISPR-activation-2A-GFP-AAVS1-targeting-construct
Plasmid#176836PurposeTargeting construct for intergrating a doxycycline (DOX) inducible SAM CRISPR activation complex -2A-GFP to the AAVS1 locus.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdCas9-VP64-2A-MS2-p65-HSF1-2A-GFP
UseUnspecifiedMutationSee Depositor Comments BelowPromoterTet operator (tetracycline inducible expression)Available sinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHS0810 pcDNA3.1(+) CMV-SV40 NLS-OgeuIscB-nucleoplasmin NLS-3xHA
Plasmid#176540PurposeMammalian expression of nuclear localized OgeuIscBDepositorInsertOgeuIscB
TagsNucleoplasmin NLS-3xHA and SV40 NLSExpressionMammalianAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
spNW80
Plasmid#173487PurposeExpresses spNW80 in Bl21DepositorInsertspNW80
TagsHistagExpressionBacterialAvailable sinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
CaMKII-somBiPOLES-mCerulean (AAV5)
Viral prep#154948-AAV5PurposeReady-to-use AAV5 particles produced from CaMKII-somBiPOLES-mCerulean (#154948). In addition to the viral particles, you will also receive purified CaMKII-somBiPOLES-mCerulean plasmid DNA. CamKII-driven expression of soma-targeted BiPOLES for optogenetic inhibition (blue light) and activation (red light). These AAV preparations are suitable purity for injection into animals.DepositorPromoterCaMKIIa(0.4)TagsmCeruleanAvailable sinceSept. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaM-4
Plasmid#172770PurposeBacterial expression of anti-mCherry nanobody LaM-4, with pelB leader and C-term free cysteine and 6xHIS tag.DepositorInsertLaM-4
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
EFSp-GFP-Empty
Plasmid#174171PurposeControl lentiviral vector expressing GFP under control of the EFS promoter.DepositorTypeEmpty backboneUseLentiviralExpressionMammalianPromoterEFSAvailable sinceAug. 19, 2021AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pYPQ131-tRNA2.0
Plasmid#158393PurposeGolden Gate entry vector to express the 1st gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoterDepositorTypeEmpty backboneUseCRISPRExpressionPlantPromoterWithout promoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
PGK plasmid
Plasmid#169744PurposeUnregulated "open-loop" PGK promoter plasmid. This plasmid encodes eGFP to report circuit output levels.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserteGFP
UseSynthetic BiologyExpressionMammalianPromoterPGK promoterAvailable sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
NanoLuc-PM
Plasmid#164784PurposeNanoluciferase (NanoLuc) targeted to the extracellular surface of the cell membrane for bioluminescence resonance energy transfer (BRET)-based measurement of ligand binding to unmodified receptorsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNanoluciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta (PDGFRB Human, Oplophorus gracilirostris)
TagsNanoLucExpressionMammalianPromoterCMVAvailable sinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-FLuc
Plasmid#170575PurposeExpress firefly luciferase. Used in MLV-based SARS-CoV-2 pseudovirus assay.DepositorInsertLuc
UseLuciferase and RetroviralTagsNAExpressionMammalianPromoterCMVAvailable sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA-NES-PhoCl2f-mCherry
Plasmid#164048PurposeOptogenetic control of protein localization by PhoCl2fDepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNES-PhoCl2f-mCherry
Tagskozak sequenceExpressionMammalianPromoterCMV promotorAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJYDC1
Plasmid#162458PurposeYeast display plasmid for expression of C-terminal fusions with Aga2p yeast agglutinin. The reporter, eUnaG2 bilirubin dependent green-yellow fluorescent protein, is fused to the NterminusDepositorPublicationTypeEmpty backboneUseShuttle vector bacteria/yeastTagsHA tag, HDEL sequence, eUnaG2 fluorescent protein…Available sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-Ef1a-Con/Foff 2.0-EYFP (AAV8)
Viral prep#137162-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-Ef1a-Con/Foff 2.0-EYFP (#137162). In addition to the viral particles, you will also receive purified pAAV-Ef1a-Con/Foff 2.0-EYFP plasmid DNA. EF1a-driven expression of EYFP in the presence of Cre (inhibited in the presence of Flp). These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsEYFP (Cre-dependent)Available sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-CCO-IRES-Puromycin
Plasmid#134861PurposeExpresses EGFP-tagged inner mitochondrial membrane markerDepositorInsertcytochrome c oxidase subunit VIII (COX8A Human)
UseLentiviralTagsEGFPExpressionMammalianPromoterEF1aAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pW34
Plasmid#158210PurposeLuxCDABE operon (R6K ori) under T7, in loxP and lox5171 sites.DepositorInsertLuxABCDE
Available sinceDec. 16, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLenti6.2_3xFLAG_V5_ mmIfih1_V5
Plasmid#160110PurposeExpresses murine Ifih1 in mammalian cellsDepositorAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (AAV8)
Viral prep#112677-AAV8PurposeReady-to-use AAV8 particles produced from pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (#112677). In addition to the viral particles, you will also receive purified pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP plasmid DNA. EF1a-driven, Cre-dependent color switch. This AAV directs expression of nuclear mCherry in the absence of Cre. In the presence of Cre, nuclear EGFP (and not mCherry) will be expressed. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsmCherry (Cre-negative cells), EGFP (Cre-positive cells)Available sinceSept. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLIX403-ccdB-G418
Plasmid#158561PurposeSet of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusionsDepositorTypeEmpty backboneUseLentiviralExpressionMammalianAvailable sinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-hSyn-nucGFP
Plasmid#140190PurposeOverexpression of GFP with a nuclear localization sequence, under the human synapsin promoterDepositorInsertGFP-NLS
UseLentiviralTagsGFPExpressionMammalianAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by