We narrowed to 59,942 results for: %s

1. pSPL3-hRPH3A_c.444Gwt

   Plasmid

   #190476

   Purpose

   minigene assay

   Depositor

   Alfredo Brusco

   Article

   Pavinato et al Genet Med. 2023 Nov;25(11):100922. doi:

   Insert

   RPH3A (NM_014954.3) c.444 [exon 7 and part of surrounding introns only] (RPH3A Human)
   

   Expression

   Mammalian

   Promoter

   SV40

   Available Since

   Sept. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-N-HA-NEK7

   Plasmid

   #75142

   Purpose

   Expresses N-terminal HA-tagged NEK7 in mammalian cells

   Depositor

   Bruce Beutler

   Article

   Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

   Insert

   NIMA (never in mitosis gene a)-related expressed kinase 7 (Nek7 Mouse)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFBOH-MHL.RING1B

   Plasmid

   #224712

   Purpose

   Expresses N-terminal hexahistidine-tagged human RING1B (RNF2) in insect cells.

   Depositor

   Chen Davidovich

   Article

   Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

   Insert

   RING1b (RNF2 Human)
   

   Tags

   6x histidine tag

   Expression

   Insect

   Promoter

   Polyhedrin

   Available Since

   Oct. 18, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pcDNA3-N-Flag-ASC1

   Plasmid

   #75134

   Purpose

   Expresses N-terminal Flag-tagged ASC1 in mammalian cells

   Depositor

   Bruce Beutler

   Article

   Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

   Insert

   PYD and CARD domain containing (Pycard Mouse)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   F23S (please see depositor comments below)

   Promoter

   CMV

   Available Since

   Aug. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYeDP60-hATP13A2-E343A

   Plasmid

   #171378

   Purpose

   A modified pYEDP60 plasmid containing a yeast codon-optimized version of human ATP13A2 variant 2 E343A mutant, followed by a thrombin cleavage site and a C-term BAD tag. DOI: 10.21769/BioProtoc.3888

   Depositor

   Peter Vangheluwe

   Article

   van Veen et al Nature. 2020 Feb;578(7795):419-424. doi:

   Insert

   human ATP13A2 variant 2 wild-type
   

   Tags

   BAD tag

   Expression

   Yeast

   Mutation

   E343A

   Promoter

   URA3

   Available Since

   Sept. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV enAsCas12f-HKRA-sgRNA_v7

   Plasmid

   #208357

   Purpose

   Expression of enAsCas12f-HKRA and sgRNA_v7 in mammalian cells

   Depositor

   Atsushi Hoshino

   Article

   Hino et al Cell. 2023 Sep 22:S0092-8674(23)00963-7.

   Insert

   enAsCas12f-HKRA
   

   Expression

   Mammalian

   Mutation

   I123H/D195K/D208R/V232A

   Promoter

   CMV

   Available Since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCROPseq-VEX-optimized-scaffold

   Plasmid

   #203321

   Purpose

   Modified CROPseq vector for efficient CRISPR screens in hematopoiesis

   Depositor

   Vijay Sankaran

   Article

   Martin-Rufino et al Cell. 2023 Apr 27:S0092-8674(23)00332-X.

   Insert

   sgRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNCS-mGold2t

   Plasmid

   #231766

   Purpose

   Expression of mGold2t in bacterial cells

   Depositor

   Francois St-Pierre

   Article

   Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

   Insert

   mGold2t
   

   Expression

   Bacterial

   Mutation

   mGold2t is mGold with V1A;V22I;H77N;Q80R;K101E;D1…

   Promoter

   Synthetic promoter

   Available Since

   March 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. A*24:02:01

   Plasmid

   #203295

   Purpose

   Overexpress HLA allele

   Depositor

   Derin Keskin, Catherine Wu

   Article

   Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

   Insert

   A*24:02:01 (HLA-A Human)
   

   Expression

   Mammalian

   Available Since

   July 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV:ITR-EF1aCore-SaCas9-dual(U6-sgRNA(backbone))-ITR

    Plasmid

    #207878

    Purpose

    AAV vector encoding EF1a core promoter-driven SaCas9 and two sgRNA cloning sites w/ the engineered Sa Guide scaffold variant (BbsI and PaqCI for sgRNA cloning).

    Depositor

    Yeh-Hsing Lao

    Article

    Lao et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Available Since

    Oct. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. PCR4-Shh::lacZ-H11

    Plasmid

    #139098

    Purpose

    enSERT LacZ reporter vector for site-specific integration into the H11 locus (contains Shh minimal promoter)

    Depositor

    Len Pennacchio

    Article

    Kvon et al Cell. 2020 Mar 6. pii: S0092-8674(20)302

    Type

    Empty backbone

    Use

    CRISPR and Mouse Targeting

    Expression

    Mammalian

    Promoter

    Shh

    Available Since

    March 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLVX-2xFLAG-2xSTREP-CCND3-IRES-mCherry

    Plasmid

    #172620

    Purpose

    Lentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D3 and mCherry in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND3 (CCND3 Human)
    

    Use

    Lentiviral

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1(+)-C-6His-mEsrra

    Plasmid

    #173152

    Purpose

    mouse estrogen-related receptor alpha (Esrra) expression plasmid in mammalian cells

    Depositor

    Brijesh Kumar Singh

    Article

    Tripathi et al bioRxiv 2021.11.16.468891

    Insert

    ORF clone Estrogen-related receptor alpha (Esrra) (Esrra Mouse)
    

    Tags

    His

    Expression

    Mammalian

    Mutation

    Wild type

    Available Since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA5 CMV 3xFG eIF4G1

    Plasmid

    #158775

    Purpose

    pcDNA5 transfection plasmid for the exogenous expression of N-terminal 3xFlag-tagged eIF4G1 isoform lacking the microexon.

    Depositor

    Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1

    Insert

    eIF4G1
    

    Tags

    3xFlag

    Expression

    Mammalian

    Available Since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMVtrunc msfGFPΔC9-LifeactΔN2

    Plasmid

    #231556

    Purpose

    Mammalian expression of N-terminally truncated Lifeact fused to C-terminally truncated monomeric superfolder GFP for actin filament organization measurements using fluorescence polarization microscopy

    Depositor

    Manos Mavrakis

    Article

    Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

    Insert

    Lifeact (ABP140 Budding Yeast)
    

    Tags

    msfGFP

    Expression

    Mammalian

    Promoter

    CMVtrunc

    Available Since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. FUW-TetO-lox-ERAS

    Plasmid

    #52417

    Purpose

    Doxycycline inducible lentiviral vector of human ERAS cDNA with excisable insert

    Depositor

    Jacob Hanna

    Article

    Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

    Insert

    ERAS (ERAS Human)
    

    Use

    Lentiviral

    Available Since

    Sept. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV enAsCas12f-YHAM-sgRNA_v7

    Plasmid

    #208356

    Purpose

    Expression of enAsCas12f-YHAM and sgRNA_v7 in mammalian cells

    Depositor

    Atsushi Hoshino

    Article

    Hino et al Cell. 2023 Sep 22:S0092-8674(23)00963-7.

    Insert

    enAsCas12f-YHAM
    

    Expression

    Mammalian

    Mutation

    F48Y/S188H/V232A/E316M

    Promoter

    CMV

    Available Since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAV:ITR-EF1aCore-SaCas9-single(U6-sgRNA(backbone))-ITR

    Plasmid

    #207880

    Purpose

    AAV vector encoding EF1a core promoter-driven SaCas9 and single sgRNA cloning site w/ the engineered Sa Guide scaffold variant (BbsI for sgRNA cloning).

    Depositor

    Yeh-Hsing Lao

    Article

    Lao et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Available Since

    Nov. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. mEGFP-N1-YAP

    Plasmid

    #166457

    Purpose

    Expresses fusion of mEGFP and YAP

    Depositor

    Shuguo Sun

    Article

    Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

    Insert

    mEGFP, YAP (YAP1 Human)
    

    Expression

    Mammalian

    Available Since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLKO-Tet-On-YAP1-shRNA1

    Plasmid

    #176846

    Purpose

    lentiviral vector for doxycycline-inducible human YAP1 knockdown

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    YAP1-Sh1 (YAP1 Human)
    

    Use

    Lentiviral

    Available Since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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