We narrowed to 8,836 results for: construct
-
Plasmid#205463PurposeA backbone containing a RsDddA fragment A for constructing mitochondria-targeted RsDdCBE expression plasmidDepositorInsertMTS; N136; ccdb; RsDddA-A; UGI
ExpressionMammalianAvailable SinceAug. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-CCDB-N136-RsDddA-C-UGI-PURO
Plasmid#205465PurposeA backbone containing a RsDddA fragment C for constructing mitochondria-targeted RsDdCBE expression plasmidDepositorInsertMTS; N136; ccdb; RsDddA-C; UGI
ExpressionMammalianAvailable SinceAug. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKIF1C-BioID2-HA
Plasmid#130980PurposeExpression of HA-tagged KIF1C-BioID2DepositorInsertKIF1C-BioID2-HA (KIF1C Human)
TagsBioID2-HAExpressionMammalianMutation5 silent mutations that make this construct RNAi …PromoterCMVAvailable SinceOct. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV2_hSyn_Aurora_Citrine
Plasmid#98217PurposeRed-shifted artificial anion conducting channelrhodopsin (aACR). Activation up to 600 nm; off-kinetics 260 ms. Codon optimized for mammalian expression.DepositorInsertSynthetic construct Aurora gene
UseAAVTagsCitrineExpressionMammalianMutationV59S, E83N, E90Q, E101S, V117R, E123S, P242R, A24…Promoterhuman synapsinAvailable SinceAug. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV2_hSyn_iChloC_CA_Citrine
Plasmid#98220PurposeSlow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with blue to green light, inactivation max with 605 nm (accelarates closure to ms). Codon optimized for mammalian expression.DepositorInsertSynthetic construct iChloC_C128A gene
UseAAVTagsCitrineExpressionMammalianMutationE83Q, E90R, E101S, C128A, T159C, D156NPromoterhuman synapsinAvailable SinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRB144
Plasmid#180175PurposepCDNA3.1 Construct GluN1 A714LDepositorAvailable SinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
Gal4-VP16-HSF1 WT
Plasmid#71726Purposeplasmid expressing fusion construct of Gal4 BDB VP16 AD and HSF1 WT RDDepositorInsertGal4 DBD HSF1 VP16 AD (HSF1 Budding Yeast, Herpes Simplex Virus protein VP16, Human)
ExpressionMammalianAvailable SinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDEST15.1-hRPN13 (285-386)
Plasmid#61936PurposeExpression of hRPN13 (285-386) in E.coliDepositorInserthRPN13 (285-386) (ADRM1 Human)
TagsGSTExpressionBacterialMutationConstruct starts at residue 285, stop codon intro…PromoterT7Available SinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_FUT8-1/1
Plasmid#91270PurposeProtein expression and purification of human SH3 domain construct FUT8-1/1DepositorInsertFUT8-1/1 (FUT8 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_ARHGEF6-1/1
Plasmid#91515PurposeProtein expression and purification of human SH3 domain construct ARHGEF6-1/1DepositorInsertARHGEF6-1/1 (ARHGEF6 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
p2T CAG dCas9-10XGcn4-P2A-mCherry BlastR
Plasmid#186745PurposedCas9-10XGcn4-P2A-mCherry expression constructDepositorInsertCas9-10XGcn4-P2A-mCherry
UseCRISPRExpressionMammalianAvailable SinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSL012_AAVS1-puro-9xTetO-pEF-H2B-mCitrine-5kb,lambda-pRSV-H2B-mCherry
Plasmid#179429PurposeDual fluorescent reporter construct with 5kb lambda spacer, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locusDepositorInsertTRE-cit-5kb-mch
ExpressionMammalianPromoterpEF alpha, pRSVAvailable SinceFeb. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
DNMT1 KO Neo
Plasmid#16649DepositorInsertDNMT1 knock-out construct (DNMT1 Human)
ExpressionBacterialAvailable SinceApril 24, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET29-hMIGA2(306-593)-6xhis
Plasmid#192837PurposeExpress codon-optimized human MIGA2 fragment 306-593 with a C-terminal 6xhis tag (lipid transfer construct) in bacteriaDepositorInsertCodon-optimized human Mitoguardin 2 (MIGA2 Human)
Tags6xhisExpressionBacterialPromoterT7Available SinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
ECON
Plasmid#82553PurposeEpigenetic effector construct for targeted methylationDepositorTagsHALO and mCherryExpressionMammalianMutationN-terminal photolyase homology region domain of C…PromoterCMVAvailable SinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCIBN-tagBFP-hNCL
Plasmid#103805PurposeBLInCR 'Localizer' construct that marks the nucleoli and is targeted by a PHR-tagged effector upon illumination with blue lightDepositorExpressionMammalianMutationhNCL: A395G (K132R), A421G (K141E), A424G (K142E)…PromoterCMVAvailable SinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_DOCK2-1/1
Plasmid#91482PurposeProtein expression and purification of human SH3 domain construct DOCK2-1/1DepositorInsertDOCK2-1/1 (DOCK2 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_AMPH-1/1
Plasmid#91556PurposeProtein expression and purification of human SH3 domain construct AMPH-1/1DepositorInsertAMPH-1/1 (AMPH Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_DBNL-1/1
Plasmid#91409PurposeProtein expression and purification of human SH3 domain construct DBNL-1/1DepositorInsertDBNL-1/1 (DBNL Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJMP1106
Plasmid#119249Purposeconstruction intermediateDepositorInsertsgRNA NT1/RR1 (rfp)
ExpressionBacterialMutationD10A and H840AAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only