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pQCXIP-Rx3(A8)-mEGFP

Plasmid

#35532

Depositor

Robert Cohen

Article

Sims et al Nat Methods. 2012 Feb 5. doi: 10.1038/nm

Insert

Rx3A8mEGFP

Use

Retroviral

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 6, 2012

Availability

Academic Institutions and Nonprofits only

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pLXMA L2A

Plasmid

#153360

Purpose

Expression vector for plants with medium-copy number origin of replication

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Type

Empty backbone

Expression

Plant

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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LI D-E YFP + linker c1

Plasmid

#54411

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

16 aa GS linker + YFP Venus

Use

Cloning vector

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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pICH50581

Plasmid

#50256

Depositor

Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter, act2 (AT3G18780, Arabidopsis thaliana)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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Farnesylated SE pcDNA3.1

Plasmid

#79666

Purpose

Expresses super ecliptic A227D in mammalian cell membranes

Depositor

Bradley Baker

Article

Kang et al Sci Rep. 2016 Apr 4;6:23865. doi: 10.103

Insert

superEcliptic pHluorin

Expression

Mammalian

Available Since

July 27, 2016

Availability

Academic Institutions and Nonprofits only

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LI F-G Hygro c1

Plasmid

#54463

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

hygromycin phosphotranferase (hptII) (pCAMBIA)

Use

Cloning vector

Available Since

June 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICH41402

Plasmid

#50285

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

5'UTR, omega (Tobacco Mosaic Virus)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pCF713

Plasmid

#121665

Purpose

U6-sgRNA EF1a-ProCas9-FlaviS6-P2A-Puro

Depositor

David Savage

Article

Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

Inserts

Use

Lentiviral

Promoter

U6

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pFH69

Plasmid

#128189

Purpose

Level1 ZmUBIp::ABE7.10 base changer (human codon optimized):NOSt

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

ZmUBIp::ABE7.10 base changer (human codon optimized):NOSt

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-gap16-ClopHensor

Plasmid

#26006

Depositor

Daniele Arosio

Article

Arosio et al Nat Methods. 2010 Jul . 7(7):516-8.

Insert

gap16-ClopHensor

Tags

Gap16 and Strep-II

Expression

Mammalian

Mutation

N-term (16AA) of Gap-43

Available Since

Sept. 16, 2010

Availability

Academic Institutions and Nonprofits only

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pOGG050

Plasmid

#113999

Purpose

SC module. Thermostable beta-galactosidase reporter

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

celB

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available Since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pOGG083

Plasmid

#114001

Purpose

SC module. Beta-glucosidase reporter

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

gusA

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available Since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

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LI C-D 1-10GFP

Plasmid

#54461

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

superfolder GFP 1-10 domain

Use

Cloning vector

Available Since

Nov. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pPTK013-3a-Invertase-αMFΔ

Plasmid

#84972

Purpose

Type 3a, Invertase followed by αMFΔ - secretion signal

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

Invertase followed by αMFΔ secretion tag

Use

Synthetic Biology

Expression

Yeast

Promoter

Non

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pRC246

Plasmid

#123038

Purpose

Core promoter

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

pLEU2m [2b]

Expression

Yeast

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-PGAL1-HBH

Plasmid

#26885

Depositor

Peter Kaiser

Article

Tagwerker et al Yeast. 2006 Jun . 23(8):623-32.

Insert

HBH tag N-terminal

Use

Pcr tagging yeast

Available Since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only

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pPTK016-3-RFP_Intra

Plasmid

#84975

Purpose

Type 3, Red fluorescent protein

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

Red fluorescent protein

Use

Synthetic Biology

Expression

Yeast

Mutation

BsaI site removed (1677 c to g and 1794 a to g)

Promoter

Non

Available Since

March 3, 2017

Availability

Academic Institutions and Nonprofits only

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pDest490-OPN-10 kDa

Plasmid

#17595

Depositor

Xin Wang

Article

Takafuji et al Oncogene. 2007 Sep 27. 26(44):6361-71.

Insert

OPN-10 kDa (SPP1 Human)

Tags

Flag

Expression

Mammalian

Mutation

Base pairs 499–630

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only
pDest490-OPN-CT

Plasmid

#17593

Depositor

Xin Wang

Article

Takafuji et al Oncogene. 2007 Sep 27. 26(44):6361-71.

Insert

OPN-CT (SPP1 Human)

Tags

Flag

Expression

Mammalian

Mutation

Base pairs 631–942

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only
pFH37

Plasmid

#126011

Purpose

Level0 Rice OsU6-2 Pol III promoter with transcription Start-G

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

Level0 Rice OsU6-2 Pol III promoter with transcription Start-G

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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