We narrowed to 22,864 results for: Sis

1. Lenti-RNF2-ngRNA+41_EF1a-puroR

   Plasmid

   #207358

   Purpose

   Lentiviral transfer plasmid encoding hU6-driven expression of a RNF2 nicking guide RNA and EF1a-driven puromycin resistance gene.

   Depositor

   Marianne Carlon

   Article

   Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

   Insert

   RNF2 +41 ngRNA
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMXS-IRES-Blast ACSL4

   Plasmid

   #173164

   Purpose

   Retroviral vector to express sgRNA resistant human ACSL4

   Depositor

   Kivanc Birsoy

   Article

   Zhu et al Mol Cell. 2019 Apr 4;74(1):45-58.e7. doi

   Insert

   Acyl-CoA Synthetase Long Chain Family Member 4 (ACSL4 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Synonymous mutations at sgRNA sites

   Promoter

   MMLV

   Available Since

   Aug. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMXS-IRES-Blast HA-GPAT4

   Plasmid

   #173168

   Purpose

   Retroviral vector to express sgRNA resistant HA tagged human GPAT4

   Depositor

   Kivanc Birsoy

   Article

   Zhu et al Mol Cell. 2019 Apr 4;74(1):45-58.e7. doi

   Insert

   Glycerol-3-Phosphate Acyltransferase 4 (GPAT4 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mutation

   Synonymous mutations at sgRNA sites

   Promoter

   MMLV

   Available Since

   Aug. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCAGGS-PHFF-sh-hBVRA

   Plasmid

   #100285

   Purpose

   Expression vector for phycocyanobilin (PCB) synthesis and shRNA for human BVRA gene in mammalian cells

   Depositor

   Kazuhiro Aoki

   Article

   Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

   Inserts

   MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Myc-Fd-P2A-MTS-Fnr-T7 (synthetic genes)
   shRNA for human BVRA
   

   Use

   Tags

   FLAG, T7 and Mitochondria-targeting sequence (MTS…

   Expression

   Mammalian

   Mutation

   All genes are codon-optimized for expression in h…

   Promoter

   CAG promoter and H1 promoter

   Available Since

   Sept. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pNOC_dfnCas12a-KRAB_crRNA NC

   Plasmid

   #176262

   Purpose

   pNOC episomal plasmid harboring the dead version of humanized fnCas12a gene sequence tagged to the KRAB domain and Nlux and spacer sequence is replaced by the type IIS restriction site for endonucleas

   Depositor

   John van der Oost

   Article

   Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

   Insert

   humanized fnCas12a
   

   Use

   CRISPR, Luciferase, and Synthetic Biology ; Expre…

   Tags

   Kruppel associated box domain and luciferase

   Expression

   Mutation

   D917A and E1006A mutations to inactivate the endo…

   Promoter

   Available Since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3-N-HA-NEK7 K64M

   Plasmid

   #75143

   Purpose

   Expresses N-terminal HA-tagged NEK7 (with a K64M substitution; kinase dead) in mammalian cells

   Depositor

   Bruce Beutler

   Article

   Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

   Insert

   NIMA (never in mitosis gene a)-related expressed kinase 7 (Nek7 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Lys 64 mutated to Met; kinase inactive and F236L …

   Promoter

   CMV

   Available Since

   May 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pNOC_dCas9-KRAB_sgRNA Td2

   Plasmid

   #176264

   Purpose

   pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged to the KRAB domain and Nlux and sgRNA targeting the fluorescent gene tdtomato with spacer 2

   Depositor

   John van der Oost

   Article

   Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

   Insert

   dead spCas9
   

   Use

   CRISPR, Luciferase, and Synthetic Biology ; Expre…

   Tags

   Kruppel associated box domain and luciferase

   Expression

   Mutation

   H840A and D10A mutations on spCas9 to inactivate …

   Promoter

   Available Since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. hEF1α-H2B-mOrange2-IRES-EGFP PGK-Puromycin

   Plasmid

   #99277

   Purpose

   Dual fluorescent reporter - constitutive expression of mOrange2 (549/565) localized in the nucleus, EGFP (488/507) untargeted and visualized in the cytoplasm, and Puromycin resistance

   Depositor

   Jordan Miller

   Article

   Calderon et al Biomater Sci. 2017 Jun 29. doi: 10.1039/

   Inserts

   H2B-mOrange2-IRES-EGFP
   Puro
   

   Use

   Lentiviral

   Tags

   EGFP, H2B, IRES, PGK, and mOrange2

   Expression

   Mutation

   Promoter

   EF1 alpha-human and PGK-mouse

   Available Since

   Nov. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pUC19-Top2A-5X Gly-Venus-T2A-Hygro

   Plasmid

   #211115

   Purpose

   For tagging the C-term of Top2A with Venus

   Depositor

   Job Dekker

   Article

   Hildebrand et al Mol Cell. 2024 Mar 13:S1097-2765(24)0014

   Inserts

   5' C-term Top2A homology arm (TOP2A Human)
   Venus
   T2A
   Blastocidin Resistance
   5' C-term Top2A homology arm (TOP2A Human)
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   n/a

   Available Since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMDC32B-AtTAS1c-D2-B/c-AtMIR173

    Plasmid

    #137885

    Purpose

    Plant expression vector for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor downstream 3'D1[+]. Contains AtMIR173 for syntasi expression in any plant species.

    Depositor

    Alberto Carbonell

    Article

    Lopez-Dolz et al Nucleic Acids Res. 2020 May 12. pii: 583

    Inserts

    AtTAS1c-D2-B/c
    2x35S-AtMIR173-Tnos
    

    Use

    Tags

    Expression

    Plant

    Mutation

    A. thaliana TAS1c precursor sequence including a …

    Promoter

    Available Since

    May 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge