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scAAV-Mlc2v-MPRA

Plasmid

#182649

Purpose

Self complementary AAV vector for high-throughput in vivo measurement of enhancer activity by massively parallel reporter assay

Depositor

William Pu

Article

Akerberg et al Nat Commun. 2019 Oct 28;10(1):4907. doi:

Insert

mCherry

Use

AAV

Expression

Mammalian

Promoter

rat Mlc2v

Available Since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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HT110_FCMV_QuasAr6b_citrine

Plasmid

#178823

Purpose

Expresses an archaerhodopsin-derived genetically encoded voltage indicator QuasAr6b carrying a Citrine expression tag

Depositor

Adam Cohen

Article

Tian et al Nat Methods. 2023 Jul;20(7):1082-1094. d

Insert

QuasAr6b_citrine

Use

Lentiviral

Tags

Citrine

Expression

Mammalian

Promoter

CMV

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pGV3319

Plasmid

#170277

Purpose

Expression of mutant Bst polymerase (Bst-LF D720A) for loop mediated isothermal amplification (LAMP)

Depositor

Gregory Van Duyne

Article

Sherrill-Mix et al Genome Biol. 2021 Jun 3;22(1):169. doi:

Insert

Bst-LF D720A

Tags

Hexahistidine

Expression

Bacterial

Mutation

D720A

Promoter

T7-lac

Available Since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

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R989-X02-566: His6-MBP-tev-G-Hs.KRAS4b(1-169)

Plasmid

#159539

Purpose

E. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-169)

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-G-Hs.KRAS4b(1-169) (KRAS Human)

Tags

His6-MBP-tev

Expression

Bacterial

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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R989-X08-566: His6-MBP-tev-G-Hs.KRAS4b(1-169) G12C

Plasmid

#159540

Purpose

E. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-169) G12C

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-G-Hs.KRAS4b(1-169) G12C (KRAS Human)

Tags

His6-MBP-tev

Expression

Bacterial

Mutation

G12C

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pETM33_Nsp10

Plasmid

#156479

Purpose

Bacterial expression of Sars-CoV2 Nsp10 protein with His-tag and GST-tag

Depositor

Ylva Ivarsson

Article

Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

Insert

Nsp10 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)

Tags

His-GST

Expression

Bacterial

Mutation

Gene insert is codon optimized for Ecoli.

Promoter

T7/LacO

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2-NONO 40

Plasmid

#127653

Purpose

Knock-out of human NONO

Depositor

Nicolas Manel

Article

Lahaye et al Cell. 2018 Oct 4;175(2):488-501.e22. doi

Insert

NONO sgRNA (NONO Human)

Use

Lentiviral

Available Since

July 9, 2019

Availability

Academic Institutions and Nonprofits only

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pLX303-PCDH7-d

Plasmid

#108541

Purpose

Expresses human PCDH7 transcript variant d in mammalian cells

Depositor

Kathryn O’Donnell

Article

Zhou et al Cancer Res. 2017 Jan 1;77(1):187-197. do

Insert

PCDH7 transcript variant d (PCDH7 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

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pMEL10

Plasmid

#107916

Purpose

URA3 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

Depositor

Jean-Marc Daran

Article

Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

Insert

gRNA-CAN1.Y

Use

CRISPR

Expression

Yeast

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pPKm-248

Plasmid

#90504

Purpose

Expresses HO1, PCYA, FD and FNR, required for PCB synthesis. pSIN - EF-1alpha - MTS - tPCYA - IRES - MTS - tHO1 - P2A - MTS - tFD - P2A - MTS - tFNR

Depositor

Todd Coleman

Article

Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

Insert

mito-tHO1, mito-tPCYA, mito-tFD, mito-tFNR

Use

Lentiviral

Available Since

April 4, 2018

Availability

Academic Institutions and Nonprofits only

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IMPT-6213

Plasmid

#99333

Purpose

AT2R N terminal BRIL, linker GSGS, delta aa 1-34, delta aa 336-363

Depositor

Raymond Stevens

Article

Zhang et al Nature. 2017 Apr 20;544(7650):327-332. d

Insert

AGTR2 (AGTR2 Human)

Expression

Insect

Promoter

polyhedrin

Available Since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pCS2-Opto-BMPR2a

Plasmid

#207616

Purpose

Zebrafish BMPR2a BMP receptor kinase domain and CTD fused to VfLOV

Depositor

Patrick Müller

Article

Rogers et al Elife. 2020 Nov 11;9:e58641. doi: 10.755

Insert

BMPR2a kinase domain and CTD + VfLOV domain (bmpr2a Vaucheria frigida, Zebrafish)

Available Since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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MSCV-human CSFI-R-L301S-IRES-GFP

Plasmid

#133744

Purpose

retroviral expression of CSF1-R mutant

Depositor

Martine Roussel

Article

Roussel et al Nature. 1987 Feb 5-11;325(6104):549-52.

Insert

CSF1-R (CSF1R Human)

Use

Retroviral

Expression

Mammalian

Mutation

L301S

Promoter

MSCV

Available Since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

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plko-puromycin-shTRX1-259

Plasmid

#110921

Purpose

To knockdown Thioredoxin-1

Depositor

Priyamvada Rai

Article

Young et al Biochem Biophys Res Commun. 2010 Feb 12;

Insert

TRX1 (TXN Human)

Use

Lentiviral

Promoter

unknown

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pEB2-mRuby3 Addgene

Plasmid

#104005

Purpose

Plasmid encoding mRuby3 Addgene

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mRuby3 Addgene

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to eqFP611 (MSKGEE LIKENMRMKVV…

Promoter

proC

Available Since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pEB2-DsRed-Express2

Plasmid

#103999

Purpose

Plasmid encoding DsRed-Express2

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

DsRed-Express2

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to DsRed (R2D, S4T, K5E, E10P,…

Promoter

proC

Available Since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-alpha2-chimaerin

Plasmid

#59316

Purpose

Expression plasmid for GFP-tagged alpha2-chimaerin (GFP at N-terminus of alpha2-chimaerin)

Depositor

Peter Scheiffele

Article

Beg et al Neuron. 2007 Sep 6;55(5):768-78.

Insert

alpha2-chimaerin

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only

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EYFP-NLS-actin-K68/284R

Plasmid

#60616

Purpose

Encodes YFP and NLS tagged beta-actin with sumoylation resistant mutations

Depositor

Primal de Lanerolle

Article

Hofmann et al J Cell Biol. 2009 Jul 27;186(2):193-200.

Insert

Beta-actin (ACTB Human)

Tags

EYFP and NLS

Expression

Mammalian

Mutation

K68R, 284R

Available Since

Nov. 21, 2014

Availability

Academic Institutions and Nonprofits only
pFA6a-kanMX6-P81nmt1-3HA

Plasmid

#39285

Depositor

Jurg Bahler, John Pringle

Article

Bahler et al Yeast. 1998 Jul;14(10):943-51.

Insert

nmt1 promoter (nmt1 Fission Yeast)

Use

Yeast genomic targeting

Tags

3xHA, A. gossypii translation elongation factor 1…

Mutation

-1163 to +6 of nmt1 locus with a 7bp deletion (AT…

Available Since

Aug. 14, 2012

Availability

Academic Institutions and Nonprofits only
pMIIS793

Plasmid

#238206

Purpose

Expresses TaTasR with an N-terminal Twin-Strep-SUMO tag. KanR

Depositor

Feng Zhang

Article

Faure et al Science. 2025 Feb 27:eadv9789. doi: 10.1

Insert

TaTasR

Tags

TwinStrepII-bdSUMO

Expression

Bacterial

Available Since

May 20, 2025

Availability

Academic Institutions and Nonprofits only

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