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Showing: 7881 - 7900 of 9412 results

  1. hsp70: mKate2 dynactin1-811

    Plasmid
    #105970
    Purpose
    heat shock inducible transgenesis, first coiled coil domain plus some more amino acids, truncated before second coiled coil domain, it's first 811 amino acids of dynactin subunit p150 glued, has dominant negative effect on dynein function
    Depositor
    Caren Norden
    Article
    Taverna et al Sci Rep. 2016 Feb 16;6:21206. doi: 10.10
    Insert
    dynactin1-811 (DCTN1 Human)
    Tags
    mKate2
    Expression
    Bacterial
    Mutation
    contains only amino acids 1-811
    Available Since
    July 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    105970-plasmid-map-sequence-id-203781

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  2. pKK-BI16-ORF1-3C-FLAG_ORF2-TEV-mClover3

    Plasmid
    #105803
    Purpose
    Concomitant expression of two proteins. One protein expressed with FLAG, cleavable by 3C protease; second protein expressed with mClover3, cleavable by TEV protease; tags positions: C-termini.
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    ORF1: 3C-FLAG; ORF2: TEV-mClover3
    Expression
    Mammalian
    Available Since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105803-plasmid-map-sequence-id-203399

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  16. pABD1275

    Plasmid
    #68017
    Purpose
    PIF1 protein overproduction for purification from bacterial cells
    Depositor
    Bruce Downie
    Article
    Majee et al Proc Natl Acad Sci U S A. 2018 Apr 24;11
    Insert
    PHY-INTERACTING FACTOR 1 (PIF1) (PIL5 AT2G20180, Mustard Weed)
    Tags
    6x-His
    Expression
    Bacterial
    Mutation
    Codon Optimized for bacterial expression (GenScri…
    Promoter
    T7
    Available Since
    Dec. 17, 2018
    Availability
    Academic Institutions and Nonprofits only

  17. pDONR223 SARS-CoV-2 ORF7B C-trunc_nostop

    Plasmid
    #153956
    Purpose
    Gateway-compatible Entry vector, with insert of truncated ORF7B CDS from SARS-CoV-2 genomic analysis in Kim et al. 2020
    Depositor
    Fritz Roth
    Article
    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4
    Insert
    ORF7B (ORF7b SARS-CoV-2 isolate Wuhan-Hu-1)
    Use
    Gateway-compatible entry vector
    Mutation
    Many synonymous changes due to codon optimization
    Available Since
    June 11, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153956-plasmid-map-sequence-id-299762

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  18. pDONR223 SARS-CoV-2 ORF7B C-trunc

    Plasmid
    #153957
    Purpose
    Gateway-compatible Entry vector, with insert of truncated ORF7B CDS from SARS-CoV-2 genomic analysis in Kim et al. 2020
    Depositor
    Fritz Roth
    Article
    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4
    Insert
    ORF7B (ORF7b SARS-CoV-2 isolate Wuhan-Hu-1)
    Use
    Gateway-compatible entry vector
    Mutation
    Many synonymous changes due to codon optimization
    Available Since
    June 9, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153957-plasmid-map-sequence-id-299766

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  20. pKK-FRET-ORF1-3C-Cerulean_ORF2-TEV-Amber

    Plasmid
    #105806
    Purpose
    Concomitant expression of two proteins. One protein expressed with Cerulean, cleavable by 3C; second protein expressed with Amber, cleavable by TEV. Control for protein interaction analysis by FRET.
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    ORF1: 3C-Cerulean; ORF2: TEV-Amber
    Expression
    Mammalian
    Available Since
    May 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    105806-plasmid-map-sequence-id-207907

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Showing: 7881 - 7900 of 9412 results