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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 25,288 results for: crispr

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Showing: 7901 - 7920 of 25288 results

  1. pCRI006-pYFAC-riboB-PgpdA-dSpCas9-VPR-TtrpC

    Plasmid
    #140199
    Purpose
    Episomal expression of dSpCas9-VPR. Filamentous fungi vector with AMA1 and riboB selection marker.
    Depositor
    Yit Heng Chooi
    Publication
    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dSpCas9-VPR
    Use
    CRISPR and Synthetic Biology; Expression in asper…
    Tags
    VPR (VP64-p65-Rta), NLS
    Promoter
    PgpdA
    Available since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    140199-plasmid-map-sequence-id-309723

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  8. pYZ145

    Plasmid
    #98405
    Purpose
    Donor DNA plasmid to introduce leu1 deletion in S. pombe. Linearization with Not1 digestion.
    Depositor
    Jef Boeke
    Publication
    Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.
    Inserts
    upstream homologous region to delete leu1 in genome by recombination (apc2 Fission Yeast)
    downstream homologous region to delete leu1 in genome by recombination (top2 Fission Yeast)
    Use
    CRISPR and Synthetic Biology
    Available since
    Feb. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    98405-plasmid-map-sequence-id-198862

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  9. pYZ149

    Plasmid
    #98407
    Purpose
    Donor DNA plasmid to introduce his3 deletion in S. pombe. Linearization with Not1 digestion.
    Depositor
    Jef Boeke
    Publication
    Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.
    Inserts
    upstream homologous region to delete his3 in genome by recombination (alg2 Fission Yeast)
    downstream homologous region to delete his3 in genome by recombination (cog8 Fission Yeast)
    Use
    CRISPR and Synthetic Biology
    Available since
    Feb. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    98407-plasmid-map-sequence-id-198894

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  15. pX330_DRS-1 sgRNA / hSpCas9

    Plasmid
    #172841
    Purpose
    Mammalian expression of the DRS-1 synthetic sgRNA sequence under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.
    Depositor
    Haining Zhong
    Publication
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DRS-1 sgRNA under the U6 promotor and hSpCas9 under the CAG promotor
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172841-plasmid-map-sequence-id-341031

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  17. NLS-nCas9-NLS-P2A-EGFP (pRZ70)

    Plasmid
    #123616
    Purpose
    CMV promoter expression plasmid for codon-optimized bpNLS-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP (ABEmax control without TadA domains).
    Depositor
    Keith Joung
    Publication
    Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NLS-nCas9-NLS-P2A-EGFP
    Expression
    Mammalian
    Mutation
    D10A in SpCas9
    Promoter
    CMV
    Available since
    April 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    123616-plasmid-map-sequence-id-239344

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  18. pCMV-eNme2-C-BE4max

    Plasmid
    #183679
    Purpose
    Expresses eNme2-C-BE4 in mammalian cells
    Depositor
    David Liu
    Publication
    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    rAPOBEC1-neNme2-C-2xUGI
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Nme2Cas9 P6S/D16A/E33G/K104T/D152A/F260L/A263T/A3…
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    183679-plasmid-map-sequence-id-374493

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  20. pJ609-dCas9-KR-puro

    Plasmid
    #158478
    Purpose
    Express nuclease dead Cas9 fused to KillerRed chromophore in mammalian cells
    Depositor
    Brendan Price
    Publication
    House et al PLoS One. 2020 Dec 17;15(12):e0237759. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-KR
    Use
    CRISPR
    Tags
    KillerRed
    Expression
    Mammalian
    Mutation
    Cas9m4 (D10A+D839A+H840A+N863A)
    Promoter
    CMV
    Available since
    Nov. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    158478-plasmid-map-sequence-id-317143

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Showing: 7901 - 7920 of 25288 results