We narrowed to 29,276 results for: tat
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Plasmid#47446PurposeAAV TALE synthesis backbone with HD half repeat; fused to nuclear localization sequence linker and VP64 transcriptional activator effector domain. Synapsin promoter for neuronal expression.DepositorInsertTALE BB(N136_0.5HD_C63)-VP64
UseAAV; TaleTags2A_GFPExpressionMammalianPromoterhSyn human synapsin (mammalian neuronal expressio…Available SinceOct. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn_TALEBB(NI)-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpA
Plasmid#47452PurposeAAV TALE synthesis backbone with NI half repeat; fused to nuclear localization sequence linker and SID4X transcriptional repressor effector domain. Synapsin promoter for neuronal expression.DepositorInsertTALE BB(N136_0.5NI_C63)-SID4X
UseAAV; TaleTags2A_phiLOV2.1ExpressionMammalianPromoterhSyn human synapsin (mammalian neuronal expressio…Available SinceOct. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)MBII-85-5'/3'ss mut
Plasmid#67645PurposeMBII85 snoRNA expression cassett, contains snoRNA in natural intron sorounded by natural exons. To improve snoRNA expression efficiency 5' and 3' splice sites were mutated to consensus sequences.DepositorInsertMBII85 snoRNA
ExpressionMammalianMutation5' and 3' splice sites mutationsAvailable SinceAug. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S(RBSmut)_Psyn-sgRNAmreB
Plasmid#149594Purposeall-in-one CRISPRi vector for targeting B. burgdorferi mreBDepositorInsertdCas9, lacI, sgRNAmreB
UseCRISPRExpressionBacterialPromoterPflaB, PpQE30, PsynAvailable SinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBGC155-MXMT
Plasmid#79818PurposeGFP(S65T)-based BiFC vector for plants (Positive control: MXMT)DepositorInsertCaMXMT1
UseReporter plasmidTagsGC155/GFP (155–239)ExpressionPlantPromoterCaMV 35S promoter and CaMV35SAvailable SinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2/V5 hsa-mir-483
Plasmid#26315DepositorInserthsa-mir-483 (MIR483 Human)
ExpressionMammalianAvailable SinceOct. 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MBP-(CBX1)3-eReader
Plasmid#135090PurposeBacterial expression of engineered CBX1 fusions with enhanced binding to H3K9meDepositorInsertCBX1 chromobox protein 1, engineered x3
Tags6xhis-tag and MBPExpressionBacterialMutationlysine 43 mutated to alanine; aspartic acid 59 mu…PromoterT7Available SinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDIV490
Plasmid#177698PurposePlasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species.DepositorInsertsCas9
Nat
UseCRISPRTagsSV40ExpressionBacterial and YeastPromoterRHR2p (Debaryomyces hansenii ) and TDH3p (Candida…Available SinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUB6_EPB49
Plasmid#84015PurposeEPB49 minigene to test splicing eventDepositorAvailable SinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn_TALEBB(NN)-NLS-VP64_2A_GFP_WPRE_bGHpA
Plasmid#47445PurposeAAV TALE synthesis backbone with NN half repeat; fused to nuclear localization sequence linker and VP64 transcriptional activator effector domain. Synapsin promoter for neuronal expression.DepositorInsertTALE BB(N136_0.5NN_C63)-VP64
UseAAV; TaleTags2A_GFPExpressionMammalianPromoterhSyn human synapsin (mammalian neuronal expressio…Available SinceOct. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTH353-SUP45wt
Plasmid#29371DepositorInsertSUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
ExpressionYeastMutationWild type gene including genomic sequence 1432 bp…Available SinceMay 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-FLAG-SBP-STAG2 (S97X)
Plasmid#73964PurposeSTAG2 cDNA (CCDS43990) S97XDepositorAvailable SinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T2-Snf1-cat
Plasmid#52683Purposeexpression of the Snf1 kinase domain in bacteriaDepositorInsertSNF1 (SNF1 Budding Yeast)
TagsGSTExpressionBacterialMutationencodes kinase domain only, aa 41-315PromotertacAvailable SinceApril 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1‐Afu‐rnhB D101N
Plasmid#108695PurposeFor expression in E. coli, and affinity purification of N-terminally GST-tagged Archaeoglobus fulgidus RNase HII with D101N catalytic site mutationDepositorInsertrnhB
TagsGSTExpressionBacterialMutationD101N catalytic site mutationPromotertacAvailable SinceApril 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJMP1274
Plasmid#119266PurposeB. subtilis deltaTn7 site and flanking sequence cloned into pACYCDepositorInsertB. subtilis deltaTn7
ExpressionBacterialAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMKO.1-shPrc1
Plasmid#160959PurposePrc1 shRNA in pMKO.1 retroviral vectorDepositorAvailable SinceDec. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
LITE1.0_pAAV_hSyn_CRY2PHR-NLS-VP64_2A_GFP_WPRE_bGHpA
Plasmid#48253PurposeLITE1.0 CRY2PHR fused to VP64 transcriptional activator domain. Binds to CIB1 upon blue light stimulation. Synapsin promoter for neuronal expression. See LITE2.0 for optimized LITE activators.DepositorInsertCRY2PHR-NLS-VP64
UseAAV; TaleTags2A_GFPExpressionMammalianPromoterEF1-alphaAvailable SinceOct. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
YFP(1-158) in pcDNAI/Amp
Plasmid#60888PurposeThis vector attaches YFP(1-158) to the N-terminus of a protein.DepositorInsertYFP(1-158)
ExpressionMammalianMutationMet was substituted for Gln-69.PromoterCMVAvailable SinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
HA-VHL T157I-pBabe-puro
Plasmid#19240DepositorInsertvon Hippel-Lindau tumor suppressor (VHL) (VHL Human)
UseRetroviralTagsHA-tagExpressionMammalianMutationchanged Threonin 157 to IsoleucinAvailable SinceOct. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
HA-VHL A149T-pBabe-puro
Plasmid#19242DepositorInsertvon Hippel-Lindau tumor suppressor (VHL) (VHL Human)
UseRetroviralTagsHA-tagExpressionMammalianMutationchanged Alanin 149 to ThreoninAvailable SinceOct. 1, 2008AvailabilityAcademic Institutions and Nonprofits only