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pUASp YFP Rab10 CA

Plasmid

#37710

Depositor

Matthew Scott

Publication

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab10 (Rab10 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

Q68L (constitutive active)

Promoter

GAL4

Available since

Nov. 28, 2012

Availability

Academic Institutions and Nonprofits only
pUASp YFP Rab8 CA

Plasmid

#37707

Depositor

Matthew Scott

Publication

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab8 (Rab8 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

Q67L (constitutive active)

Promoter

GAL4

Available since

Nov. 16, 2012

Availability

Academic Institutions and Nonprofits only
pUASp YFP Rab8 DN

Plasmid

#37706

Depositor

Matthew Scott

Publication

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab8 (Rab8 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

T22N (dominant negative)

Promoter

GAL4

Available since

Nov. 16, 2012

Availability

Academic Institutions and Nonprofits only
pUASp YFP Rab6 CA

Plasmid

#37698

Depositor

Matthew Scott

Publication

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab6 (Rab6 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

Q71L (constitutive active)

Promoter

GAL4

Available since

Nov. 16, 2012

Availability

Academic Institutions and Nonprofits only
pUASp YFP Rab6 DN

Plasmid

#37697

Depositor

Matthew Scott

Publication

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab6 (Rab6 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

T26N (dominant negative)

Promoter

GAL4

Available since

Nov. 16, 2012

Availability

Academic Institutions and Nonprofits only
pUASp YFP Rab3 WT

Plasmid

#37687

Depositor

Matthew Scott

Publication

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab3 (Rab3 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Promoter

GAL4

Available since

Nov. 16, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pCMV_POLD2-APOBEC1-UdgX-nCas9-UdgX

Plasmid

#163543

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Publication

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

POLD2-APOBEC1-UdgX-nCas9-UdgX

Use

CRISPR

Expression

Mammalian

Mutation

nCas9 (D10A)

Available since

Dec. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pDHJS3 AS-

Plasmid

#78237

Purpose

One of four plasmids needed to create the double Holliday junction substrate (DHJS).

Depositor

Tao-shih Hsieh

Publication

Plank et al J Biol Chem. 2006 Jun 23;281(25):17510-6

Inserts

loxP
sequence "B"
loxP2

Use

Phagemid

Available since

Sept. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag TAOK3

Plasmid

#20647

Depositor

William Hahn, Jean Zhao

Publication

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

TAOK3 (TAOK3 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag TYK2

Plasmid

#20660

Depositor

William Hahn, Jean Zhao

Publication

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

TYK2 (TYK2 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag AKT3

Plasmid

#20423

Depositor

William Hahn, Jean Zhao

Publication

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

AKT3 (AKT3 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pHR-CD80

Plasmid

#223603

Purpose

Lentiviral expression of human CD80 in mammalian cells

Depositor

Enfu Hui

Publication

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD80 (CD80 Human)

Use

Lentiviral; mammalian expression

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pN256

Plasmid

#188472

Purpose

scDAISY-seq lentiviral construct encoding crRNA targeting the DAISY chain that is within the UTR of eGFP

Depositor

Le Cong

Publication

Hughes et al Mol Cell. 2022 Jun 22. pii: S1097-2765(2

Insert

eGFP-WPRE-DAISY-4T

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pUFlip-floxed2A-mRFP-; gcry1:BFP -2

Plasmid

#173888

Purpose

Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

Depositor

Jeffrey Essner

Publication

Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

Insert

2A-mRFP; gcry1: BFP

Use

Synthetic Biology

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pUFlip-floxed2A-mRFP-; gcry1:BFP -0

Plasmid

#173886

Purpose

Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

Depositor

Jeffrey Essner

Publication

Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

Insert

2A-mRFP; gcry1: BFP

Use

Synthetic Biology

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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P-rTetO-EGFP-T2A-RPL22-3xHA

Plasmid

#170326

Purpose

Tetracycline-inducible expression of HA and EGFP tagged RPL22

Depositor

Hemali Phatnani

Publication

Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

Insert

RPL22 (RPL22 Human)

Use

Lentiviral

Mutation

None

Available since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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MSCV-Tox2-IRES-eGFP

Plasmid

#165428

Purpose

Expresses TOX2 in mammalian cells

Depositor

Patrick Hogan, Anjana Rao

Publication

Seo et al Proc Natl Acad Sci U S A. 2019 Jun 18;11

Insert

TOX2 (Tox2 Mouse)

Tags

3xflag 5' of Tox2

Expression

Mammalian

Promoter

5' LTR

Available since

March 3, 2021

Availability

Academic Institutions and Nonprofits only

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pLentiRNACRISPR_002 - hU6-DR_BsmBI-EFS-PguCas13b-NES-2A-Puro-WPRE

Plasmid

#138144

Purpose

Expresses PguCas13b in mammalian cells, localized in the cytoplasm. For cloning of guide RNAs compatible with PguCas13b. Contains a 3' direct repeat. Clone using BsmBI. F overhang cacc. R overhang caac

Depositor

Neville Sanjana

Publication

Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

Insert

PguCas13b

Use

Lentiviral

Tags

NES

Available since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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LLP249 pGK-dCas9-DNMT3a V3

Plasmid

#100938

Purpose

dCas9 and DNMT WT driven by pGK promoter and Puromycin driven by EF1a

Depositor

Ryan Lister

Publication

Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

Insert

dCas9, DNMT3a catalytic domain (DNMT3A S.pyogenes, Human)

Use

CRISPR and Lentiviral

Tags

3xHA and 3xTy1

Expression

Mammalian

Mutation

D10A, H840A for dCas9

Available since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pTMB341_ZFcharm_Prnp_SCR

Plasmid

#220844

Purpose

AAV genome expressing ZFcharm targeting Prnp; scrambled self-silencing binding site

Depositor

Jonathan Weissman

Publication

Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

Insert

ZFcharm

Use

AAV

Tags

HAtag

Expression

Mammalian

Mutation

N/A

Promoter

EFS

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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